A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes

Autores
Baudin, Maël; Laloum, Tom; Lepage, Agnes; Rípodas, Carolina; Ariel, Federico Damian; Frances, Lisa; Crespi, Martin; Gamas, Pascal; Blanco, Flavio Antonio; Zanetti, María Eugenia; de Carvalho-Niebel, Fernanda; Niebel, Andreas
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The endosymbiotic association between legumes and rhizobia leads to the formation of root nodules in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activation of symbiotic genes that are controlled by a set of early transcription factors (TFs). MtNF-YA1 and MtNF-YA2 are two TFs playing partially redundant functions during several steps of the symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti. NF-Y proteins are part of a transcriptional complex composed of three proteins (NF-YA, NF-YB and NF-YC) which bind DNA at CCAAT-boxes, a motif present in most eukaryotic promoters. In plants, each subunit is encoded by small gene families, potentially leading to a multitude of heterotrimeric NF-Y complexes. Here, using yeast two hybrid screenings, we identified the MtNF-YB and MtNF-YC subunits that interact with MtNF-YA1 and A2. Further, we confirmed, both in yeast and in planta, the formation of trimeric NF-Y complexes and showed that these complexes are functional during nodulation using reverse genetic approaches and ChIP-PCR. Finally, as orthologs of the characterized NF-Y subunits also control nodulation in other legumes, we showed in common bean that similar NF-Y trimers could form in planta. Our results suggest that we have identified a group of evolutionary conserved NF-Y proteins that interact to control nodulation in leguminous plants.
Fil: Baudin, Maël. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Laloum, Tom. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Lepage, Agnes. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Rípodas, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre D'etudes de Saclay; Francia
Fil: Frances, Lisa. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Crespi, Martin. Centre D'etudes de Saclay; Francia
Fil: Gamas, Pascal. Centre National de la Recherche Scientifique; Francia
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: de Carvalho-Niebel, Fernanda. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Niebel, Andreas. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Materia
Transcription factors
nodulation
medicago
symbiosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48109
Acceder
id CONICETDig_37320fb87e1e73da8278f8f8cd523511
oai_identifier_str oai:ri.conicet.gov.ar:11336/48109
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumesBaudin, MaëlLaloum, TomLepage, AgnesRípodas, CarolinaAriel, Federico DamianFrances, LisaCrespi, MartinGamas, PascalBlanco, Flavio AntonioZanetti, María Eugeniade Carvalho-Niebel, FernandaNiebel, AndreasTranscription factorsnodulationmedicagosymbiosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The endosymbiotic association between legumes and rhizobia leads to the formation of root nodules in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activation of symbiotic genes that are controlled by a set of early transcription factors (TFs). MtNF-YA1 and MtNF-YA2 are two TFs playing partially redundant functions during several steps of the symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti. NF-Y proteins are part of a transcriptional complex composed of three proteins (NF-YA, NF-YB and NF-YC) which bind DNA at CCAAT-boxes, a motif present in most eukaryotic promoters. In plants, each subunit is encoded by small gene families, potentially leading to a multitude of heterotrimeric NF-Y complexes. Here, using yeast two hybrid screenings, we identified the MtNF-YB and MtNF-YC subunits that interact with MtNF-YA1 and A2. Further, we confirmed, both in yeast and in planta, the formation of trimeric NF-Y complexes and showed that these complexes are functional during nodulation using reverse genetic approaches and ChIP-PCR. Finally, as orthologs of the characterized NF-Y subunits also control nodulation in other legumes, we showed in common bean that similar NF-Y trimers could form in planta. Our results suggest that we have identified a group of evolutionary conserved NF-Y proteins that interact to control nodulation in leguminous plants.Fil: Baudin, Maël. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Laloum, Tom. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Lepage, Agnes. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Rípodas, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre D'etudes de Saclay; FranciaFil: Frances, Lisa. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Crespi, Martin. Centre D'etudes de Saclay; FranciaFil: Gamas, Pascal. Centre National de la Recherche Scientifique; FranciaFil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: de Carvalho-Niebel, Fernanda. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Niebel, Andreas. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaAmerican Society of Plant Biologist2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48109Baudin, Maël; Laloum, Tom; Lepage, Agnes; Rípodas, Carolina; Ariel, Federico Damian; et al.; A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes; American Society of Plant Biologist; Plant Physiology; 169; 10-2015; 2761-27730032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.01144info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/169/4/2761info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:23Zoai:ri.conicet.gov.ar:11336/48109instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:23.7CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
title A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
spellingShingle A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
Baudin, Maël
Transcription factors
nodulation
medicago
symbiosis
title_short A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
title_full A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
title_fullStr A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
title_full_unstemmed A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
title_sort A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
dc.creator.none.fl_str_mv Baudin, Maël
Laloum, Tom
Lepage, Agnes
Rípodas, Carolina
Ariel, Federico Damian
Frances, Lisa
Crespi, Martin
Gamas, Pascal
Blanco, Flavio Antonio
Zanetti, María Eugenia
de Carvalho-Niebel, Fernanda
Niebel, Andreas
author Baudin, Maël
author_facet Baudin, Maël
Laloum, Tom
Lepage, Agnes
Rípodas, Carolina
Ariel, Federico Damian
Frances, Lisa
Crespi, Martin
Gamas, Pascal
Blanco, Flavio Antonio
Zanetti, María Eugenia
de Carvalho-Niebel, Fernanda
Niebel, Andreas
author_role author
author2 Laloum, Tom
Lepage, Agnes
Rípodas, Carolina
Ariel, Federico Damian
Frances, Lisa
Crespi, Martin
Gamas, Pascal
Blanco, Flavio Antonio
Zanetti, María Eugenia
de Carvalho-Niebel, Fernanda
Niebel, Andreas
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Transcription factors
nodulation
medicago
symbiosis
topic Transcription factors
nodulation
medicago
symbiosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The endosymbiotic association between legumes and rhizobia leads to the formation of root nodules in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activation of symbiotic genes that are controlled by a set of early transcription factors (TFs). MtNF-YA1 and MtNF-YA2 are two TFs playing partially redundant functions during several steps of the symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti. NF-Y proteins are part of a transcriptional complex composed of three proteins (NF-YA, NF-YB and NF-YC) which bind DNA at CCAAT-boxes, a motif present in most eukaryotic promoters. In plants, each subunit is encoded by small gene families, potentially leading to a multitude of heterotrimeric NF-Y complexes. Here, using yeast two hybrid screenings, we identified the MtNF-YB and MtNF-YC subunits that interact with MtNF-YA1 and A2. Further, we confirmed, both in yeast and in planta, the formation of trimeric NF-Y complexes and showed that these complexes are functional during nodulation using reverse genetic approaches and ChIP-PCR. Finally, as orthologs of the characterized NF-Y subunits also control nodulation in other legumes, we showed in common bean that similar NF-Y trimers could form in planta. Our results suggest that we have identified a group of evolutionary conserved NF-Y proteins that interact to control nodulation in leguminous plants.
Fil: Baudin, Maël. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Laloum, Tom. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Lepage, Agnes. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Rípodas, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre D'etudes de Saclay; Francia
Fil: Frances, Lisa. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Crespi, Martin. Centre D'etudes de Saclay; Francia
Fil: Gamas, Pascal. Centre National de la Recherche Scientifique; Francia
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: de Carvalho-Niebel, Fernanda. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Niebel, Andreas. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
description The endosymbiotic association between legumes and rhizobia leads to the formation of root nodules in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activation of symbiotic genes that are controlled by a set of early transcription factors (TFs). MtNF-YA1 and MtNF-YA2 are two TFs playing partially redundant functions during several steps of the symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti. NF-Y proteins are part of a transcriptional complex composed of three proteins (NF-YA, NF-YB and NF-YC) which bind DNA at CCAAT-boxes, a motif present in most eukaryotic promoters. In plants, each subunit is encoded by small gene families, potentially leading to a multitude of heterotrimeric NF-Y complexes. Here, using yeast two hybrid screenings, we identified the MtNF-YB and MtNF-YC subunits that interact with MtNF-YA1 and A2. Further, we confirmed, both in yeast and in planta, the formation of trimeric NF-Y complexes and showed that these complexes are functional during nodulation using reverse genetic approaches and ChIP-PCR. Finally, as orthologs of the characterized NF-Y subunits also control nodulation in other legumes, we showed in common bean that similar NF-Y trimers could form in planta. Our results suggest that we have identified a group of evolutionary conserved NF-Y proteins that interact to control nodulation in leguminous plants.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48109
Baudin, Maël; Laloum, Tom; Lepage, Agnes; Rípodas, Carolina; Ariel, Federico Damian; et al.; A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes; American Society of Plant Biologist; Plant Physiology; 169; 10-2015; 2761-2773
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48109
identifier_str_mv Baudin, Maël; Laloum, Tom; Lepage, Agnes; Rípodas, Carolina; Ariel, Federico Damian; et al.; A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes; American Society of Plant Biologist; Plant Physiology; 169; 10-2015; 2761-2773
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.01144
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/169/4/2761
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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