Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits

Autores
Pesce, Virginia Mercedes; Nally, Maria Cristina; Carrizo, Gabriela P.; Rojo, María Cecilia; Perez, Beatriz Alida; Toro, Maria Eugenia; Castellanos, Lucia Ines; Vazquez, Fabio
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the present study were to evaluate in vitro biocontrol strategies against C. gloeosporioides, in order to select native antagonistic yeasts isolated from different microenvironments, and to verify the in vivo biocontrol activity on ripe olive fruits. The potential human pathogenicity of the antagonistic yeasts was also considered. A total of 241 wild yeasts from diverse olive cultures and vitivinicultural microenvironments were assayed in vitro and in vivo for their antagonistic effect against the pathogen C. gloeosporioides. Ninety-two yeasts with in vitro characteristics of competitiveness such as niche overlap, dominance against the pathogen, reduction of fungal growth and production of antifungal metabolites were examined on ripe olive fruits. Nine biocontrol yeasts, three from fermenting grape must and six from olive fruits, leaves and branches in vivo reduced the incidence and severity of C. gloeosporioides. According to the 42 °C test, which assays for pseudohyphal formation and phospholipase production, these biocontrol yeasts did not present a health risk to humans. Microenvironments with selective stress factors can be relevant sources of microorganisms with biocontrol purposes.
Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrizo, Gabriela P.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Beatriz Alida. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Materia
Biocontrol
Yeast
Olive
Colletotrichum
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42875
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42875
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruitsPesce, Virginia MercedesNally, Maria CristinaCarrizo, Gabriela P.Rojo, María CeciliaPerez, Beatriz AlidaToro, Maria EugeniaCastellanos, Lucia InesVazquez, FabioBiocontrolYeastOliveColletotrichumhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the present study were to evaluate in vitro biocontrol strategies against C. gloeosporioides, in order to select native antagonistic yeasts isolated from different microenvironments, and to verify the in vivo biocontrol activity on ripe olive fruits. The potential human pathogenicity of the antagonistic yeasts was also considered. A total of 241 wild yeasts from diverse olive cultures and vitivinicultural microenvironments were assayed in vitro and in vivo for their antagonistic effect against the pathogen C. gloeosporioides. Ninety-two yeasts with in vitro characteristics of competitiveness such as niche overlap, dominance against the pathogen, reduction of fungal growth and production of antifungal metabolites were examined on ripe olive fruits. Nine biocontrol yeasts, three from fermenting grape must and six from olive fruits, leaves and branches in vivo reduced the incidence and severity of C. gloeosporioides. According to the 42 °C test, which assays for pseudohyphal formation and phospholipase production, these biocontrol yeasts did not present a health risk to humans. Microenvironments with selective stress factors can be relevant sources of microorganisms with biocontrol purposes.Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carrizo, Gabriela P.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez, Beatriz Alida. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaAcademic Press Inc Elsevier Science2018-05info:eu-repo/date/embargoEnd/2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42875Pesce, Virginia Mercedes; Nally, Maria Cristina; Carrizo, Gabriela P.; Rojo, María Cecilia; Perez, Beatriz Alida; et al.; Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits; Academic Press Inc Elsevier Science; Biological Control; 120; 5-2018; 43-511049-9644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2017.03.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1049964417300531info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:18Zoai:ri.conicet.gov.ar:11336/42875instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:19.236CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
title Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
spellingShingle Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
Pesce, Virginia Mercedes
Biocontrol
Yeast
Olive
Colletotrichum
title_short Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
title_full Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
title_fullStr Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
title_full_unstemmed Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
title_sort Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
dc.creator.none.fl_str_mv Pesce, Virginia Mercedes
Nally, Maria Cristina
Carrizo, Gabriela P.
Rojo, María Cecilia
Perez, Beatriz Alida
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author Pesce, Virginia Mercedes
author_facet Pesce, Virginia Mercedes
Nally, Maria Cristina
Carrizo, Gabriela P.
Rojo, María Cecilia
Perez, Beatriz Alida
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author_role author
author2 Nally, Maria Cristina
Carrizo, Gabriela P.
Rojo, María Cecilia
Perez, Beatriz Alida
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biocontrol
Yeast
Olive
Colletotrichum
topic Biocontrol
Yeast
Olive
Colletotrichum
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the present study were to evaluate in vitro biocontrol strategies against C. gloeosporioides, in order to select native antagonistic yeasts isolated from different microenvironments, and to verify the in vivo biocontrol activity on ripe olive fruits. The potential human pathogenicity of the antagonistic yeasts was also considered. A total of 241 wild yeasts from diverse olive cultures and vitivinicultural microenvironments were assayed in vitro and in vivo for their antagonistic effect against the pathogen C. gloeosporioides. Ninety-two yeasts with in vitro characteristics of competitiveness such as niche overlap, dominance against the pathogen, reduction of fungal growth and production of antifungal metabolites were examined on ripe olive fruits. Nine biocontrol yeasts, three from fermenting grape must and six from olive fruits, leaves and branches in vivo reduced the incidence and severity of C. gloeosporioides. According to the 42 °C test, which assays for pseudohyphal formation and phospholipase production, these biocontrol yeasts did not present a health risk to humans. Microenvironments with selective stress factors can be relevant sources of microorganisms with biocontrol purposes.
Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrizo, Gabriela P.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Beatriz Alida. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
description Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the present study were to evaluate in vitro biocontrol strategies against C. gloeosporioides, in order to select native antagonistic yeasts isolated from different microenvironments, and to verify the in vivo biocontrol activity on ripe olive fruits. The potential human pathogenicity of the antagonistic yeasts was also considered. A total of 241 wild yeasts from diverse olive cultures and vitivinicultural microenvironments were assayed in vitro and in vivo for their antagonistic effect against the pathogen C. gloeosporioides. Ninety-two yeasts with in vitro characteristics of competitiveness such as niche overlap, dominance against the pathogen, reduction of fungal growth and production of antifungal metabolites were examined on ripe olive fruits. Nine biocontrol yeasts, three from fermenting grape must and six from olive fruits, leaves and branches in vivo reduced the incidence and severity of C. gloeosporioides. According to the 42 °C test, which assays for pseudohyphal formation and phospholipase production, these biocontrol yeasts did not present a health risk to humans. Microenvironments with selective stress factors can be relevant sources of microorganisms with biocontrol purposes.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
info:eu-repo/date/embargoEnd/2018-12-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42875
Pesce, Virginia Mercedes; Nally, Maria Cristina; Carrizo, Gabriela P.; Rojo, María Cecilia; Perez, Beatriz Alida; et al.; Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits; Academic Press Inc Elsevier Science; Biological Control; 120; 5-2018; 43-51
1049-9644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42875
identifier_str_mv Pesce, Virginia Mercedes; Nally, Maria Cristina; Carrizo, Gabriela P.; Rojo, María Cecilia; Perez, Beatriz Alida; et al.; Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits; Academic Press Inc Elsevier Science; Biological Control; 120; 5-2018; 43-51
1049-9644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2017.03.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1049964417300531
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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