First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina

Autores
Bernanrdi Lima, Nelson; Pastor, Silvina Estela; Maza, Claudia Elizabeth; Conforto, Erica Cinthia; Vargas Gil, Silvina; Roca, Monica
Año de publicación
2020
Idioma
alemán
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The olive (Olea europaea L.), family Oleaceae, is an important crop in Argentina, mainly in the production of olive oils and table olives. In the country, that economic loss to the olive industry caused by anthracnose is estimated to be over $9 million dollars a year. During the harvest of 2018 to 2019, severe symptoms of anthracnose were observed with an incidence of 73% on 483 olive tree (cv. Manzanilla) in a commercial orchard located in Capital, La Rioja, Argentina. Lesions on olive fruits were irregular, becoming dark brown and depressed, with mature fruit mummification, being typical lesions of anthracnose. For fungal isolation, conidia were collected from orange masses of spores, in acervuli, from 20 infected fruits of 10 olive tree, and were placed in Petri plates containing potato dextrose agar (PDA). Plates were incubated at 25°C in the dark for 6 days, and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA. Three isolates were obtained and then single-spore purified. The isolates (IPAVE 071, IPAVE 072, and IPAVE 076) were preserved and deposited in the Culture Collection of Instituto de Patología Vegetal (IPAVE) at the Instituto Nacional de Tecnología Agropecuaria (INTA) (Córdoba, Argentina). Colonies presented mycelium that were flat with a white margin, and gray aerial mycelium. Conidia were hyaline, aseptate, straight, subcylindrical and clavate, (12.3 to) 13.9 to 19.1 (to 20.57) × (3.5 to) 4.1 to 5.61 (to 6.1) µm, mean ± SD = 14.8 ± 0.2 × 4.8 ± 0.1 µm, length/width ratio = 3.1 (n = 50). Morphological characterization was consistent with the description of Colletotrichum theobromicola (Rojas et al. 2010). For molecular identification, gene sequences were obtained from the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and β-tubulin 2 (TUB2) regions, which were amplified by PCR (Weir et al. 2012) and sequenced.
Instituto de Patología Vegetal
Fil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.
Fil: Maza, Claudia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chilecito; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Roca, Monica. Universidad Nacional La Rioja. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fuente
Plant Disease 104 (2) : (February 2020 )
Materia
Colletotrichum
Olea Europaea
Phylogeny
Pathogenicity
Filogenia
Patogenicidad
Argentina
Colletotrichum Gloeosporioides Species Complex
Phylogenetic Analysis
Olivo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8103
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8103
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in ArgentinaBernanrdi Lima, NelsonPastor, Silvina EstelaMaza, Claudia ElizabethConforto, Erica CinthiaVargas Gil, SilvinaRoca, MonicaColletotrichumOlea EuropaeaPhylogenyPathogenicityFilogeniaPatogenicidadArgentinaColletotrichum Gloeosporioides Species ComplexPhylogenetic AnalysisOlivoThe olive (Olea europaea L.), family Oleaceae, is an important crop in Argentina, mainly in the production of olive oils and table olives. In the country, that economic loss to the olive industry caused by anthracnose is estimated to be over $9 million dollars a year. During the harvest of 2018 to 2019, severe symptoms of anthracnose were observed with an incidence of 73% on 483 olive tree (cv. Manzanilla) in a commercial orchard located in Capital, La Rioja, Argentina. Lesions on olive fruits were irregular, becoming dark brown and depressed, with mature fruit mummification, being typical lesions of anthracnose. For fungal isolation, conidia were collected from orange masses of spores, in acervuli, from 20 infected fruits of 10 olive tree, and were placed in Petri plates containing potato dextrose agar (PDA). Plates were incubated at 25°C in the dark for 6 days, and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA. Three isolates were obtained and then single-spore purified. The isolates (IPAVE 071, IPAVE 072, and IPAVE 076) were preserved and deposited in the Culture Collection of Instituto de Patología Vegetal (IPAVE) at the Instituto Nacional de Tecnología Agropecuaria (INTA) (Córdoba, Argentina). Colonies presented mycelium that were flat with a white margin, and gray aerial mycelium. Conidia were hyaline, aseptate, straight, subcylindrical and clavate, (12.3 to) 13.9 to 19.1 (to 20.57) × (3.5 to) 4.1 to 5.61 (to 6.1) µm, mean ± SD = 14.8 ± 0.2 × 4.8 ± 0.1 µm, length/width ratio = 3.1 (n = 50). Morphological characterization was consistent with the description of Colletotrichum theobromicola (Rojas et al. 2010). For molecular identification, gene sequences were obtained from the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and β-tubulin 2 (TUB2) regions, which were amplified by PCR (Weir et al. 2012) and sequenced.Instituto de Patología VegetalFil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.Fil: Maza, Claudia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chilecito; ArgentinaFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Roca, Monica. Universidad Nacional La Rioja. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaAmerican Phytopathological Society2020-10-22T11:26:36Z2020-10-22T11:26:36Z2020-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8103https://apsjournals.apsnet.org/doi/10.1094/PDIS-06-19-1207-PDN0191-29171943-7692https://doi.org/10.1094/PDIS-06-19-1207-PDNPlant Disease 104 (2) : (February 2020 )reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariadeuinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:55Zoai:localhost:20.500.12123/8103instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:55.709INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
title First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
spellingShingle First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
Bernanrdi Lima, Nelson
Colletotrichum
Olea Europaea
Phylogeny
Pathogenicity
Filogenia
Patogenicidad
Argentina
Colletotrichum Gloeosporioides Species Complex
Phylogenetic Analysis
Olivo
title_short First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
title_full First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
title_fullStr First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
title_full_unstemmed First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
title_sort First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
dc.creator.none.fl_str_mv Bernanrdi Lima, Nelson
Pastor, Silvina Estela
Maza, Claudia Elizabeth
Conforto, Erica Cinthia
Vargas Gil, Silvina
Roca, Monica
author Bernanrdi Lima, Nelson
author_facet Bernanrdi Lima, Nelson
Pastor, Silvina Estela
Maza, Claudia Elizabeth
Conforto, Erica Cinthia
Vargas Gil, Silvina
Roca, Monica
author_role author
author2 Pastor, Silvina Estela
Maza, Claudia Elizabeth
Conforto, Erica Cinthia
Vargas Gil, Silvina
Roca, Monica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Colletotrichum
Olea Europaea
Phylogeny
Pathogenicity
Filogenia
Patogenicidad
Argentina
Colletotrichum Gloeosporioides Species Complex
Phylogenetic Analysis
Olivo
topic Colletotrichum
Olea Europaea
Phylogeny
Pathogenicity
Filogenia
Patogenicidad
Argentina
Colletotrichum Gloeosporioides Species Complex
Phylogenetic Analysis
Olivo
dc.description.none.fl_txt_mv The olive (Olea europaea L.), family Oleaceae, is an important crop in Argentina, mainly in the production of olive oils and table olives. In the country, that economic loss to the olive industry caused by anthracnose is estimated to be over $9 million dollars a year. During the harvest of 2018 to 2019, severe symptoms of anthracnose were observed with an incidence of 73% on 483 olive tree (cv. Manzanilla) in a commercial orchard located in Capital, La Rioja, Argentina. Lesions on olive fruits were irregular, becoming dark brown and depressed, with mature fruit mummification, being typical lesions of anthracnose. For fungal isolation, conidia were collected from orange masses of spores, in acervuli, from 20 infected fruits of 10 olive tree, and were placed in Petri plates containing potato dextrose agar (PDA). Plates were incubated at 25°C in the dark for 6 days, and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA. Three isolates were obtained and then single-spore purified. The isolates (IPAVE 071, IPAVE 072, and IPAVE 076) were preserved and deposited in the Culture Collection of Instituto de Patología Vegetal (IPAVE) at the Instituto Nacional de Tecnología Agropecuaria (INTA) (Córdoba, Argentina). Colonies presented mycelium that were flat with a white margin, and gray aerial mycelium. Conidia were hyaline, aseptate, straight, subcylindrical and clavate, (12.3 to) 13.9 to 19.1 (to 20.57) × (3.5 to) 4.1 to 5.61 (to 6.1) µm, mean ± SD = 14.8 ± 0.2 × 4.8 ± 0.1 µm, length/width ratio = 3.1 (n = 50). Morphological characterization was consistent with the description of Colletotrichum theobromicola (Rojas et al. 2010). For molecular identification, gene sequences were obtained from the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and β-tubulin 2 (TUB2) regions, which were amplified by PCR (Weir et al. 2012) and sequenced.
Instituto de Patología Vegetal
Fil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.
Fil: Maza, Claudia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chilecito; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Roca, Monica. Universidad Nacional La Rioja. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
description The olive (Olea europaea L.), family Oleaceae, is an important crop in Argentina, mainly in the production of olive oils and table olives. In the country, that economic loss to the olive industry caused by anthracnose is estimated to be over $9 million dollars a year. During the harvest of 2018 to 2019, severe symptoms of anthracnose were observed with an incidence of 73% on 483 olive tree (cv. Manzanilla) in a commercial orchard located in Capital, La Rioja, Argentina. Lesions on olive fruits were irregular, becoming dark brown and depressed, with mature fruit mummification, being typical lesions of anthracnose. For fungal isolation, conidia were collected from orange masses of spores, in acervuli, from 20 infected fruits of 10 olive tree, and were placed in Petri plates containing potato dextrose agar (PDA). Plates were incubated at 25°C in the dark for 6 days, and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA. Three isolates were obtained and then single-spore purified. The isolates (IPAVE 071, IPAVE 072, and IPAVE 076) were preserved and deposited in the Culture Collection of Instituto de Patología Vegetal (IPAVE) at the Instituto Nacional de Tecnología Agropecuaria (INTA) (Córdoba, Argentina). Colonies presented mycelium that were flat with a white margin, and gray aerial mycelium. Conidia were hyaline, aseptate, straight, subcylindrical and clavate, (12.3 to) 13.9 to 19.1 (to 20.57) × (3.5 to) 4.1 to 5.61 (to 6.1) µm, mean ± SD = 14.8 ± 0.2 × 4.8 ± 0.1 µm, length/width ratio = 3.1 (n = 50). Morphological characterization was consistent with the description of Colletotrichum theobromicola (Rojas et al. 2010). For molecular identification, gene sequences were obtained from the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and β-tubulin 2 (TUB2) regions, which were amplified by PCR (Weir et al. 2012) and sequenced.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-22T11:26:36Z
2020-10-22T11:26:36Z
2020-01-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8103
https://apsjournals.apsnet.org/doi/10.1094/PDIS-06-19-1207-PDN
0191-2917
1943-7692
https://doi.org/10.1094/PDIS-06-19-1207-PDN
url http://hdl.handle.net/20.500.12123/8103
https://apsjournals.apsnet.org/doi/10.1094/PDIS-06-19-1207-PDN
https://doi.org/10.1094/PDIS-06-19-1207-PDN
identifier_str_mv 0191-2917
1943-7692
dc.language.none.fl_str_mv deu
language deu
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv Plant Disease 104 (2) : (February 2020 )
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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