Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts

Autores
Pesce, Virginia Mercedes; Carrizo, Gustavo Pablo; Nally, Maria Cristina; Brizuela, M.; Rodríguez Assaf, Leticia Anahí; Toro, Maria Eugenia; Castellanos, Lucia Ines; Vazquez, Fabio
Año de publicación
2015
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Anthracnose, caused by Colletotrichum sp., is the major fungal disease in all olive producing areas of the world. In Argentina, this disease was reported in Catamarca, La Rioja, Córdoba, Mendoza and San Juan. Biological control using antagonistic microorganisms has been developed as an alternative to synthetic fungicide treatment. Yeasts have characteristics that give them advantages over other microorganisms to exert biocontrol of pathogenic fungi. However, yeasts-like fungi (Aureobasidium) have been reported against Colletotrichum species in olive fruits. The aim of this work was to evaluate the ability of autochthonous yeasts to control C. gloeosporioides in mature olive fruits, at different concentrations. Methodology: 92 antagonistic indigenous yeasts were previously selected in in vitro tests: 45 yeasts from viticultural environments and 47 from olivicultural environments. An isolate of C. gloeosporioides from the collection of INTA IMyZA, Castelar was used as pathogen. The pathogenicity of C. gloeosporioides was evaluated in olive fruits. The fungus was inoculated at different concentrations (10 2 to 10 6 conidia/mL) in mature fruits, and incubated during 5 days at 25°C. Percentage incidence [(number of decayed wounds/number of total wounds) x 100] and the mean of lesion diameter were determined. A suspension of yeasts (10 8 cells/mL) and 10 5 conidia/mL of fungi were inoculated in wounded fruits for biocontrol at in vivo tests (5 days, 25°C). Wounded fruits inoculated with fungal spore suspension were included as negative controls. The incidence of disease and %severity [(mean of diameter lesion in treated fruits/mean of diameter lesion in negative control) x 100] was calculated. A minimum of 50% reduction in the incidence of the disease was considered as antagonistic activity. The influence of yeast concentration at 10 7 and 10 6 cells/mL on biocontrol activity was evaluated. All experiments were made by triplicate. Nine indigenous yeasts (Pichia membranifaciens BPm6; Saccharomyces chevalieri BSch25; Torulaspora delbrueckii BTd126; Candida tropicalis Bo13b; Cryptococcus albidus Bo86; Pichia kudriavzevii Bo91, Bo108; Wickerhamomyces anomalus Bo107, Bo156) reduced the incidence of C. gloeosporioides. Biocontrol efficacies of these yeasts were: 50%, 50%, 63.34%, 90%, 76.67%, 53.34%, 70%, 60% and 90%, respectively. All antagonistic yeasts (except W. anomalus Bo107) were significantly effective in reducing the lesion diameter at 10 8 cells/mL. Only C. tropicalis Bo13b was antagonistic at lower concentration (10 7 cells/mL) with 70% incidence reduction. It can be concluded from this study that indigenous yeasts were effective as in vivo biocontrol agents against C. gloeosporioides. Concentrations of antagonists significantly affected their biocontrol activity. This is the first report that informed autochthonous biocontrol yeasts against C. gloeosporioides in olive.
Fil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Carrizo, Gustavo Pablo. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Brizuela, M.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rodríguez Assaf, Leticia Anahí. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
XI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
Materia
Collectrotichum gloeosporioides
Olive
Biocontrol
Yeasts
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/188984
Acceder
id CONICETDig_e1e96cb468ed6971b688d040c63cd35b
oai_identifier_str oai:ri.conicet.gov.ar:11336/188984
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeastsPesce, Virginia MercedesCarrizo, Gustavo PabloNally, Maria CristinaBrizuela, M.Rodríguez Assaf, Leticia AnahíToro, Maria EugeniaCastellanos, Lucia InesVazquez, FabioCollectrotichum gloeosporioidesOliveBiocontrolYeastshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Anthracnose, caused by Colletotrichum sp., is the major fungal disease in all olive producing areas of the world. In Argentina, this disease was reported in Catamarca, La Rioja, Córdoba, Mendoza and San Juan. Biological control using antagonistic microorganisms has been developed as an alternative to synthetic fungicide treatment. Yeasts have characteristics that give them advantages over other microorganisms to exert biocontrol of pathogenic fungi. However, yeasts-like fungi (Aureobasidium) have been reported against Colletotrichum species in olive fruits. The aim of this work was to evaluate the ability of autochthonous yeasts to control C. gloeosporioides in mature olive fruits, at different concentrations. Methodology: 92 antagonistic indigenous yeasts were previously selected in in vitro tests: 45 yeasts from viticultural environments and 47 from olivicultural environments. An isolate of C. gloeosporioides from the collection of INTA IMyZA, Castelar was used as pathogen. The pathogenicity of C. gloeosporioides was evaluated in olive fruits. The fungus was inoculated at different concentrations (10 2 to 10 6 conidia/mL) in mature fruits, and incubated during 5 days at 25°C. Percentage incidence [(number of decayed wounds/number of total wounds) x 100] and the mean of lesion diameter were determined. A suspension of yeasts (10 8 cells/mL) and 10 5 conidia/mL of fungi were inoculated in wounded fruits for biocontrol at in vivo tests (5 days, 25°C). Wounded fruits inoculated with fungal spore suspension were included as negative controls. The incidence of disease and %severity [(mean of diameter lesion in treated fruits/mean of diameter lesion in negative control) x 100] was calculated. A minimum of 50% reduction in the incidence of the disease was considered as antagonistic activity. The influence of yeast concentration at 10 7 and 10 6 cells/mL on biocontrol activity was evaluated. All experiments were made by triplicate. Nine indigenous yeasts (Pichia membranifaciens BPm6; Saccharomyces chevalieri BSch25; Torulaspora delbrueckii BTd126; Candida tropicalis Bo13b; Cryptococcus albidus Bo86; Pichia kudriavzevii Bo91, Bo108; Wickerhamomyces anomalus Bo107, Bo156) reduced the incidence of C. gloeosporioides. Biocontrol efficacies of these yeasts were: 50%, 50%, 63.34%, 90%, 76.67%, 53.34%, 70%, 60% and 90%, respectively. All antagonistic yeasts (except W. anomalus Bo107) were significantly effective in reducing the lesion diameter at 10 8 cells/mL. Only C. tropicalis Bo13b was antagonistic at lower concentration (10 7 cells/mL) with 70% incidence reduction. It can be concluded from this study that indigenous yeasts were effective as in vivo biocontrol agents against C. gloeosporioides. Concentrations of antagonists significantly affected their biocontrol activity. This is the first report that informed autochthonous biocontrol yeasts against C. gloeosporioides in olive.Fil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Carrizo, Gustavo Pablo. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Brizuela, M.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Rodríguez Assaf, Leticia Anahí. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaXI Congreso Argentino de Microbiología GeneralCórdobaArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/188984Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts; XI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2015; 1-3CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2015.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:30Zoai:ri.conicet.gov.ar:11336/188984instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:30.771CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
title Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
spellingShingle Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
Pesce, Virginia Mercedes
Collectrotichum gloeosporioides
Olive
Biocontrol
Yeasts
title_short Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
title_full Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
title_fullStr Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
title_full_unstemmed Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
title_sort Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts
dc.creator.none.fl_str_mv Pesce, Virginia Mercedes
Carrizo, Gustavo Pablo
Nally, Maria Cristina
Brizuela, M.
Rodríguez Assaf, Leticia Anahí
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author Pesce, Virginia Mercedes
author_facet Pesce, Virginia Mercedes
Carrizo, Gustavo Pablo
Nally, Maria Cristina
Brizuela, M.
Rodríguez Assaf, Leticia Anahí
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author_role author
author2 Carrizo, Gustavo Pablo
Nally, Maria Cristina
Brizuela, M.
Rodríguez Assaf, Leticia Anahí
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Collectrotichum gloeosporioides
Olive
Biocontrol
Yeasts
topic Collectrotichum gloeosporioides
Olive
Biocontrol
Yeasts
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Anthracnose, caused by Colletotrichum sp., is the major fungal disease in all olive producing areas of the world. In Argentina, this disease was reported in Catamarca, La Rioja, Córdoba, Mendoza and San Juan. Biological control using antagonistic microorganisms has been developed as an alternative to synthetic fungicide treatment. Yeasts have characteristics that give them advantages over other microorganisms to exert biocontrol of pathogenic fungi. However, yeasts-like fungi (Aureobasidium) have been reported against Colletotrichum species in olive fruits. The aim of this work was to evaluate the ability of autochthonous yeasts to control C. gloeosporioides in mature olive fruits, at different concentrations. Methodology: 92 antagonistic indigenous yeasts were previously selected in in vitro tests: 45 yeasts from viticultural environments and 47 from olivicultural environments. An isolate of C. gloeosporioides from the collection of INTA IMyZA, Castelar was used as pathogen. The pathogenicity of C. gloeosporioides was evaluated in olive fruits. The fungus was inoculated at different concentrations (10 2 to 10 6 conidia/mL) in mature fruits, and incubated during 5 days at 25°C. Percentage incidence [(number of decayed wounds/number of total wounds) x 100] and the mean of lesion diameter were determined. A suspension of yeasts (10 8 cells/mL) and 10 5 conidia/mL of fungi were inoculated in wounded fruits for biocontrol at in vivo tests (5 days, 25°C). Wounded fruits inoculated with fungal spore suspension were included as negative controls. The incidence of disease and %severity [(mean of diameter lesion in treated fruits/mean of diameter lesion in negative control) x 100] was calculated. A minimum of 50% reduction in the incidence of the disease was considered as antagonistic activity. The influence of yeast concentration at 10 7 and 10 6 cells/mL on biocontrol activity was evaluated. All experiments were made by triplicate. Nine indigenous yeasts (Pichia membranifaciens BPm6; Saccharomyces chevalieri BSch25; Torulaspora delbrueckii BTd126; Candida tropicalis Bo13b; Cryptococcus albidus Bo86; Pichia kudriavzevii Bo91, Bo108; Wickerhamomyces anomalus Bo107, Bo156) reduced the incidence of C. gloeosporioides. Biocontrol efficacies of these yeasts were: 50%, 50%, 63.34%, 90%, 76.67%, 53.34%, 70%, 60% and 90%, respectively. All antagonistic yeasts (except W. anomalus Bo107) were significantly effective in reducing the lesion diameter at 10 8 cells/mL. Only C. tropicalis Bo13b was antagonistic at lower concentration (10 7 cells/mL) with 70% incidence reduction. It can be concluded from this study that indigenous yeasts were effective as in vivo biocontrol agents against C. gloeosporioides. Concentrations of antagonists significantly affected their biocontrol activity. This is the first report that informed autochthonous biocontrol yeasts against C. gloeosporioides in olive.
Fil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Carrizo, Gustavo Pablo. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Brizuela, M.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rodríguez Assaf, Leticia Anahí. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
XI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
description Anthracnose, caused by Colletotrichum sp., is the major fungal disease in all olive producing areas of the world. In Argentina, this disease was reported in Catamarca, La Rioja, Córdoba, Mendoza and San Juan. Biological control using antagonistic microorganisms has been developed as an alternative to synthetic fungicide treatment. Yeasts have characteristics that give them advantages over other microorganisms to exert biocontrol of pathogenic fungi. However, yeasts-like fungi (Aureobasidium) have been reported against Colletotrichum species in olive fruits. The aim of this work was to evaluate the ability of autochthonous yeasts to control C. gloeosporioides in mature olive fruits, at different concentrations. Methodology: 92 antagonistic indigenous yeasts were previously selected in in vitro tests: 45 yeasts from viticultural environments and 47 from olivicultural environments. An isolate of C. gloeosporioides from the collection of INTA IMyZA, Castelar was used as pathogen. The pathogenicity of C. gloeosporioides was evaluated in olive fruits. The fungus was inoculated at different concentrations (10 2 to 10 6 conidia/mL) in mature fruits, and incubated during 5 days at 25°C. Percentage incidence [(number of decayed wounds/number of total wounds) x 100] and the mean of lesion diameter were determined. A suspension of yeasts (10 8 cells/mL) and 10 5 conidia/mL of fungi were inoculated in wounded fruits for biocontrol at in vivo tests (5 days, 25°C). Wounded fruits inoculated with fungal spore suspension were included as negative controls. The incidence of disease and %severity [(mean of diameter lesion in treated fruits/mean of diameter lesion in negative control) x 100] was calculated. A minimum of 50% reduction in the incidence of the disease was considered as antagonistic activity. The influence of yeast concentration at 10 7 and 10 6 cells/mL on biocontrol activity was evaluated. All experiments were made by triplicate. Nine indigenous yeasts (Pichia membranifaciens BPm6; Saccharomyces chevalieri BSch25; Torulaspora delbrueckii BTd126; Candida tropicalis Bo13b; Cryptococcus albidus Bo86; Pichia kudriavzevii Bo91, Bo108; Wickerhamomyces anomalus Bo107, Bo156) reduced the incidence of C. gloeosporioides. Biocontrol efficacies of these yeasts were: 50%, 50%, 63.34%, 90%, 76.67%, 53.34%, 70%, 60% and 90%, respectively. All antagonistic yeasts (except W. anomalus Bo107) were significantly effective in reducing the lesion diameter at 10 8 cells/mL. Only C. tropicalis Bo13b was antagonistic at lower concentration (10 7 cells/mL) with 70% incidence reduction. It can be concluded from this study that indigenous yeasts were effective as in vivo biocontrol agents against C. gloeosporioides. Concentrations of antagonists significantly affected their biocontrol activity. This is the first report that informed autochthonous biocontrol yeasts against C. gloeosporioides in olive.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/188984
Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts; XI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2015; 1-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/188984
identifier_str_mv Biocontrol of Collectrotichum gloeosporioides in olive fruits by antagonistic yeasts; XI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2015; 1-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2015.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083382128148480
score 13.22299

Publicaciones similares