Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food

Autores
Delpech, Gastón; Schell, Celia María Beatriz; Pourcel, Natalia Gisela; Sanchez Bruni, Sergio Fabian; Tabera, Anahi; de Luca, Maria Marta; Basualdo, Juan; Sparo, Mónica Delfina
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Enterococcus spp usually showed intrinsic antimicrobial resistance to antimicrobials used in clinical practice, like cephalosporins and aminoglucosides. Vancomycin enterococcus resistant (VRE) were reported by CDC as Public Health riskinternational emergence. However, the resistance selection pressure exerted for the misuse of antibiotics at hospitals does not explain the emergence of VRE in invasive infectious diseases in susceptible or immune-deficient patients. The main research goal of this study was to investigate the presence of VRE in meat and milk derived-artisanal food. Eighteen salami and 21 cheeses, elaborated in 4 different artisanal meat-milk elaboration units (Tandil) were studied. Each sample was homogenized, processed by duplicate and inoculated in a specific VRE medium BH agar-vancomycin (6 μg mL -1). Phenotypification was assessed by conventional biochemical tests, also using the SDS-PAGE method. Besides, was investigated the associated resistance (agar diffusion) to ampicillin, teicoplanin, imipenem, linezolid, ciprofloxacin and gentamicin. The presence of VRE in artisanal food was demonstrated as follows: six VRE strains were isolated (1 E. faecalis, 3 E. faecium, 1 E. gallinarum 1 E. raffinosus) with associated resistance to ciprofloxacin, gentamicin (higher level of resistance) and linezolid. More regional studies in the search of VRE are required, since the artisanal food may act as reservoir and or vehicle of these strains, establishing an emergent concern in public health.
Fil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: Schell, Celia María Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: Tabera, Anahi. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: de Luca, Maria Marta. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Basualdo, Juan. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental
Rosario
Argentina
Sociedad Argentina de Farmacología Experimental
Materia
ENTEROCOCCUS FAECALIS CECT7121
ANTIMICROBIALS
ARTISANAL FOOD
VANCOMYCIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242056
Acceder
id CONICETDig_7ccc5a82b44c52758720ff2bf769f6d6
oai_identifier_str oai:ri.conicet.gov.ar:11336/242056
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal foodDelpech, GastónSchell, Celia María BeatrizPourcel, Natalia GiselaSanchez Bruni, Sergio FabianTabera, Anahide Luca, Maria MartaBasualdo, JuanSparo, Mónica DelfinaENTEROCOCCUS FAECALIS CECT7121ANTIMICROBIALSARTISANAL FOODVANCOMYCINhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Enterococcus spp usually showed intrinsic antimicrobial resistance to antimicrobials used in clinical practice, like cephalosporins and aminoglucosides. Vancomycin enterococcus resistant (VRE) were reported by CDC as Public Health riskinternational emergence. However, the resistance selection pressure exerted for the misuse of antibiotics at hospitals does not explain the emergence of VRE in invasive infectious diseases in susceptible or immune-deficient patients. The main research goal of this study was to investigate the presence of VRE in meat and milk derived-artisanal food. Eighteen salami and 21 cheeses, elaborated in 4 different artisanal meat-milk elaboration units (Tandil) were studied. Each sample was homogenized, processed by duplicate and inoculated in a specific VRE medium BH agar-vancomycin (6 μg mL -1). Phenotypification was assessed by conventional biochemical tests, also using the SDS-PAGE method. Besides, was investigated the associated resistance (agar diffusion) to ampicillin, teicoplanin, imipenem, linezolid, ciprofloxacin and gentamicin. The presence of VRE in artisanal food was demonstrated as follows: six VRE strains were isolated (1 E. faecalis, 3 E. faecium, 1 E. gallinarum 1 E. raffinosus) with associated resistance to ciprofloxacin, gentamicin (higher level of resistance) and linezolid. More regional studies in the search of VRE are required, since the artisanal food may act as reservoir and or vehicle of these strains, establishing an emergent concern in public health.Fil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; ArgentinaFil: Schell, Celia María Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; ArgentinaFil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; ArgentinaFil: Tabera, Anahi. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; ArgentinaFil: de Luca, Maria Marta. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Basualdo, Juan. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; ArgentinaLXI Reunion Anual de Sociedad Argentina de Farmacología ExperimentalRosarioArgentinaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de Farmacología Experimental2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/242056Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food; LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:58:28Zoai:ri.conicet.gov.ar:11336/242056instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:58:29.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
title Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
spellingShingle Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
Delpech, Gastón
ENTEROCOCCUS FAECALIS CECT7121
ANTIMICROBIALS
ARTISANAL FOOD
VANCOMYCIN
title_short Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
title_full Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
title_fullStr Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
title_full_unstemmed Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
title_sort Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food
dc.creator.none.fl_str_mv Delpech, Gastón
Schell, Celia María Beatriz
Pourcel, Natalia Gisela
Sanchez Bruni, Sergio Fabian
Tabera, Anahi
de Luca, Maria Marta
Basualdo, Juan
Sparo, Mónica Delfina
author Delpech, Gastón
author_facet Delpech, Gastón
Schell, Celia María Beatriz
Pourcel, Natalia Gisela
Sanchez Bruni, Sergio Fabian
Tabera, Anahi
de Luca, Maria Marta
Basualdo, Juan
Sparo, Mónica Delfina
author_role author
author2 Schell, Celia María Beatriz
Pourcel, Natalia Gisela
Sanchez Bruni, Sergio Fabian
Tabera, Anahi
de Luca, Maria Marta
Basualdo, Juan
Sparo, Mónica Delfina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTEROCOCCUS FAECALIS CECT7121
ANTIMICROBIALS
ARTISANAL FOOD
VANCOMYCIN
topic ENTEROCOCCUS FAECALIS CECT7121
ANTIMICROBIALS
ARTISANAL FOOD
VANCOMYCIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Enterococcus spp usually showed intrinsic antimicrobial resistance to antimicrobials used in clinical practice, like cephalosporins and aminoglucosides. Vancomycin enterococcus resistant (VRE) were reported by CDC as Public Health riskinternational emergence. However, the resistance selection pressure exerted for the misuse of antibiotics at hospitals does not explain the emergence of VRE in invasive infectious diseases in susceptible or immune-deficient patients. The main research goal of this study was to investigate the presence of VRE in meat and milk derived-artisanal food. Eighteen salami and 21 cheeses, elaborated in 4 different artisanal meat-milk elaboration units (Tandil) were studied. Each sample was homogenized, processed by duplicate and inoculated in a specific VRE medium BH agar-vancomycin (6 μg mL -1). Phenotypification was assessed by conventional biochemical tests, also using the SDS-PAGE method. Besides, was investigated the associated resistance (agar diffusion) to ampicillin, teicoplanin, imipenem, linezolid, ciprofloxacin and gentamicin. The presence of VRE in artisanal food was demonstrated as follows: six VRE strains were isolated (1 E. faecalis, 3 E. faecium, 1 E. gallinarum 1 E. raffinosus) with associated resistance to ciprofloxacin, gentamicin (higher level of resistance) and linezolid. More regional studies in the search of VRE are required, since the artisanal food may act as reservoir and or vehicle of these strains, establishing an emergent concern in public health.
Fil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: Schell, Celia María Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: Tabera, Anahi. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
Fil: de Luca, Maria Marta. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Basualdo, Juan. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud; Argentina
LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental
Rosario
Argentina
Sociedad Argentina de Farmacología Experimental
description Enterococcus spp usually showed intrinsic antimicrobial resistance to antimicrobials used in clinical practice, like cephalosporins and aminoglucosides. Vancomycin enterococcus resistant (VRE) were reported by CDC as Public Health riskinternational emergence. However, the resistance selection pressure exerted for the misuse of antibiotics at hospitals does not explain the emergence of VRE in invasive infectious diseases in susceptible or immune-deficient patients. The main research goal of this study was to investigate the presence of VRE in meat and milk derived-artisanal food. Eighteen salami and 21 cheeses, elaborated in 4 different artisanal meat-milk elaboration units (Tandil) were studied. Each sample was homogenized, processed by duplicate and inoculated in a specific VRE medium BH agar-vancomycin (6 μg mL -1). Phenotypification was assessed by conventional biochemical tests, also using the SDS-PAGE method. Besides, was investigated the associated resistance (agar diffusion) to ampicillin, teicoplanin, imipenem, linezolid, ciprofloxacin and gentamicin. The presence of VRE in artisanal food was demonstrated as follows: six VRE strains were isolated (1 E. faecalis, 3 E. faecium, 1 E. gallinarum 1 E. raffinosus) with associated resistance to ciprofloxacin, gentamicin (higher level of resistance) and linezolid. More regional studies in the search of VRE are required, since the artisanal food may act as reservoir and or vehicle of these strains, establishing an emergent concern in public health.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242056
Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food; LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242056
identifier_str_mv Characterisation of enterococcus resistant to vancomyicin in meat and milk derived artisanal food; LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782299680538624
score 12.982451

Publicaciones similares