Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat
- Autores
- Sparo, M. D.; Confalonieri, Alejandra; Urbizu, Lucia Paola; Ceci, M.; Sanchez Bruni, Sergio Fabian
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Meat and particularly ground beef is frequently associated with Food Poisoning episodes and breeches in Food Safety. The main goal of this research was to evaluate the bactericide effect of the probiotic Enterococcus faecalis CECT7121, against different pathogens as: Escherichia coli O157:H7, Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes, inoculated in ground beef meat. Three studies were performed to evaluate the inhibition of E. faecalis CECT7121 on ground beef meat samples inoculated with pathogens: Study I: Samples (100 g meat) were inoculated with pathogens (103 CFU/g)) and E. faecalis CECT7121 (104 CFU/g) simulta- neously. Study II: Samples were inoculated with E. faecalis CECT7121 24 h before the pathogens. Study III: E. faecalis CECT7121were inoculated 24 h after pathogens. The viable counts were per- formed at 0, 24, 48 and 72 h post-inoculation. The simultaneous inoculation of E. faecalis CECT7121 with E. coli O157:H7 strains resulted in the absence of viable counts of bacteria at 72 h post-treatment. However, when the probiotic was added 24 h before and 24 h after the pathogen E. coli O157:H7, viable cells were not detected at 24 h and 48 h post-treatment, respectively. Consis- tently, neither S. aureus nor Cl. perfringens viable bacteria were detected at 48 h in whole assays when inoculated with E. faecalis CECT7121. The same trend than described before was obtained af- ter applying the 3 models assayed for L. monocytogenes. The current assays demonstrated the bactericide activity of E. faecalis CECT7121 strain on bacterial pathogens in ground beef meat.
Fil: Sparo, M. D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina;
Fil: Confalonieri, Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigación Veterinaria de Tandil; Argentina;
Fil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina;
Fil: Ceci, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina;
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigación Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina; - Materia
-
Probioticos
Enfermedades infecciosas
Enterococcus Faecalis CECT7121
Alimentos - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1871
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Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meatSparo, M. D.Confalonieri, AlejandraUrbizu, Lucia PaolaCeci, M.Sanchez Bruni, Sergio FabianProbioticosEnfermedades infecciosasEnterococcus Faecalis CECT7121Alimentoshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Meat and particularly ground beef is frequently associated with Food Poisoning episodes and breeches in Food Safety. The main goal of this research was to evaluate the bactericide effect of the probiotic Enterococcus faecalis CECT7121, against different pathogens as: Escherichia coli O157:H7, Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes, inoculated in ground beef meat. Three studies were performed to evaluate the inhibition of E. faecalis CECT7121 on ground beef meat samples inoculated with pathogens: Study I: Samples (100 g meat) were inoculated with pathogens (103 CFU/g)) and E. faecalis CECT7121 (104 CFU/g) simulta- neously. Study II: Samples were inoculated with E. faecalis CECT7121 24 h before the pathogens. Study III: E. faecalis CECT7121were inoculated 24 h after pathogens. The viable counts were per- formed at 0, 24, 48 and 72 h post-inoculation. The simultaneous inoculation of E. faecalis CECT7121 with E. coli O157:H7 strains resulted in the absence of viable counts of bacteria at 72 h post-treatment. However, when the probiotic was added 24 h before and 24 h after the pathogen E. coli O157:H7, viable cells were not detected at 24 h and 48 h post-treatment, respectively. Consis- tently, neither S. aureus nor Cl. perfringens viable bacteria were detected at 48 h in whole assays when inoculated with E. faecalis CECT7121. The same trend than described before was obtained af- ter applying the 3 models assayed for L. monocytogenes. The current assays demonstrated the bactericide activity of E. faecalis CECT7121 strain on bacterial pathogens in ground beef meat.Fil: Sparo, M. D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina;Fil: Confalonieri, Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigación Veterinaria de Tandil; Argentina;Fil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina;Fil: Ceci, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina;Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigación Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina;Sociedade Brasileira de Microbiologia2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1871Sparo, M. D.; Confalonieri, Alejandra; Urbizu, Lucia Paola; Ceci, M.; Sanchez Bruni, Sergio Fabian; Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 1; 4-2013; 43-491517-83821678-4405enginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/pdf/bjm/v44n1/337806.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-83822013005000003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:02Zoai:ri.conicet.gov.ar:11336/1871instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:02.451CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat |
title |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat |
spellingShingle |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat Sparo, M. D. Probioticos Enfermedades infecciosas Enterococcus Faecalis CECT7121 Alimentos |
title_short |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat |
title_full |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat |
title_fullStr |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat |
title_full_unstemmed |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat |
title_sort |
Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat |
dc.creator.none.fl_str_mv |
Sparo, M. D. Confalonieri, Alejandra Urbizu, Lucia Paola Ceci, M. Sanchez Bruni, Sergio Fabian |
author |
Sparo, M. D. |
author_facet |
Sparo, M. D. Confalonieri, Alejandra Urbizu, Lucia Paola Ceci, M. Sanchez Bruni, Sergio Fabian |
author_role |
author |
author2 |
Confalonieri, Alejandra Urbizu, Lucia Paola Ceci, M. Sanchez Bruni, Sergio Fabian |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Probioticos Enfermedades infecciosas Enterococcus Faecalis CECT7121 Alimentos |
topic |
Probioticos Enfermedades infecciosas Enterococcus Faecalis CECT7121 Alimentos |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Meat and particularly ground beef is frequently associated with Food Poisoning episodes and breeches in Food Safety. The main goal of this research was to evaluate the bactericide effect of the probiotic Enterococcus faecalis CECT7121, against different pathogens as: Escherichia coli O157:H7, Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes, inoculated in ground beef meat. Three studies were performed to evaluate the inhibition of E. faecalis CECT7121 on ground beef meat samples inoculated with pathogens: Study I: Samples (100 g meat) were inoculated with pathogens (103 CFU/g)) and E. faecalis CECT7121 (104 CFU/g) simulta- neously. Study II: Samples were inoculated with E. faecalis CECT7121 24 h before the pathogens. Study III: E. faecalis CECT7121were inoculated 24 h after pathogens. The viable counts were per- formed at 0, 24, 48 and 72 h post-inoculation. The simultaneous inoculation of E. faecalis CECT7121 with E. coli O157:H7 strains resulted in the absence of viable counts of bacteria at 72 h post-treatment. However, when the probiotic was added 24 h before and 24 h after the pathogen E. coli O157:H7, viable cells were not detected at 24 h and 48 h post-treatment, respectively. Consis- tently, neither S. aureus nor Cl. perfringens viable bacteria were detected at 48 h in whole assays when inoculated with E. faecalis CECT7121. The same trend than described before was obtained af- ter applying the 3 models assayed for L. monocytogenes. The current assays demonstrated the bactericide activity of E. faecalis CECT7121 strain on bacterial pathogens in ground beef meat. Fil: Sparo, M. D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina; Fil: Confalonieri, Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigación Veterinaria de Tandil; Argentina; Fil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina; Fil: Ceci, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina; Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigación Veterinaria de Tandil; Argentina; Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina; |
description |
Meat and particularly ground beef is frequently associated with Food Poisoning episodes and breeches in Food Safety. The main goal of this research was to evaluate the bactericide effect of the probiotic Enterococcus faecalis CECT7121, against different pathogens as: Escherichia coli O157:H7, Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes, inoculated in ground beef meat. Three studies were performed to evaluate the inhibition of E. faecalis CECT7121 on ground beef meat samples inoculated with pathogens: Study I: Samples (100 g meat) were inoculated with pathogens (103 CFU/g)) and E. faecalis CECT7121 (104 CFU/g) simulta- neously. Study II: Samples were inoculated with E. faecalis CECT7121 24 h before the pathogens. Study III: E. faecalis CECT7121were inoculated 24 h after pathogens. The viable counts were per- formed at 0, 24, 48 and 72 h post-inoculation. The simultaneous inoculation of E. faecalis CECT7121 with E. coli O157:H7 strains resulted in the absence of viable counts of bacteria at 72 h post-treatment. However, when the probiotic was added 24 h before and 24 h after the pathogen E. coli O157:H7, viable cells were not detected at 24 h and 48 h post-treatment, respectively. Consis- tently, neither S. aureus nor Cl. perfringens viable bacteria were detected at 48 h in whole assays when inoculated with E. faecalis CECT7121. The same trend than described before was obtained af- ter applying the 3 models assayed for L. monocytogenes. The current assays demonstrated the bactericide activity of E. faecalis CECT7121 strain on bacterial pathogens in ground beef meat. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1871 Sparo, M. D.; Confalonieri, Alejandra; Urbizu, Lucia Paola; Ceci, M.; Sanchez Bruni, Sergio Fabian; Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 1; 4-2013; 43-49 1517-8382 1678-4405 |
url |
http://hdl.handle.net/11336/1871 |
identifier_str_mv |
Sparo, M. D.; Confalonieri, Alejandra; Urbizu, Lucia Paola; Ceci, M.; Sanchez Bruni, Sergio Fabian; Enterococcus faecalis CECT7121: Bio-control of pathogens in ground meat; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 1; 4-2013; 43-49 1517-8382 1678-4405 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/pdf/bjm/v44n1/337806.pdf info:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-83822013005000003 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270142403182592 |
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13.13397 |