Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining

Autores
Pezzoni, Magdalena; Pizarro, Ramón Augusto; Costa, Cristina Susana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or antibiotics, but also, according to their high resistance to standard antimicrobial approaches, alternative strategies employing photocatalysis or control of biofilm formation by modifying surfaces, have been proposed. Colony forming units (cfu) counting and live/dead staining, two classic techniques used for biofilm quantification, are detailed in this work. Both methods assess cell viability, a key factor to analyze the microbial susceptibility to given treatment, then, they represent a good approach for evaluation of an antibiofilm strategy.
Fil: Pezzoni, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Materia
PSEUDOMONAS AERUGINOSA
VIABILITY
BIOFILM
LIVE/DEAD STAINING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138270
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead stainingPezzoni, MagdalenaPizarro, Ramón AugustoCosta, Cristina SusanaPSEUDOMONAS AERUGINOSAVIABILITYBIOFILMLIVE/DEAD STAININGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or antibiotics, but also, according to their high resistance to standard antimicrobial approaches, alternative strategies employing photocatalysis or control of biofilm formation by modifying surfaces, have been proposed. Colony forming units (cfu) counting and live/dead staining, two classic techniques used for biofilm quantification, are detailed in this work. Both methods assess cell viability, a key factor to analyze the microbial susceptibility to given treatment, then, they represent a good approach for evaluation of an antibiofilm strategy.Fil: Pezzoni, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaBio-protocol2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138270Pezzoni, Magdalena; Pizarro, Ramón Augusto; Costa, Cristina Susana; Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining; Bio-protocol; Bio-Protocol; 10; 18; 9-2020; 1-122331-8325CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e3762info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.3762info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:07Zoai:ri.conicet.gov.ar:11336/138270instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:07.875CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
title Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
spellingShingle Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
Pezzoni, Magdalena
PSEUDOMONAS AERUGINOSA
VIABILITY
BIOFILM
LIVE/DEAD STAINING
title_short Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
title_full Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
title_fullStr Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
title_full_unstemmed Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
title_sort Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
dc.creator.none.fl_str_mv Pezzoni, Magdalena
Pizarro, Ramón Augusto
Costa, Cristina Susana
author Pezzoni, Magdalena
author_facet Pezzoni, Magdalena
Pizarro, Ramón Augusto
Costa, Cristina Susana
author_role author
author2 Pizarro, Ramón Augusto
Costa, Cristina Susana
author2_role author
author
dc.subject.none.fl_str_mv PSEUDOMONAS AERUGINOSA
VIABILITY
BIOFILM
LIVE/DEAD STAINING
topic PSEUDOMONAS AERUGINOSA
VIABILITY
BIOFILM
LIVE/DEAD STAINING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or antibiotics, but also, according to their high resistance to standard antimicrobial approaches, alternative strategies employing photocatalysis or control of biofilm formation by modifying surfaces, have been proposed. Colony forming units (cfu) counting and live/dead staining, two classic techniques used for biofilm quantification, are detailed in this work. Both methods assess cell viability, a key factor to analyze the microbial susceptibility to given treatment, then, they represent a good approach for evaluation of an antibiofilm strategy.
Fil: Pezzoni, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
description Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or antibiotics, but also, according to their high resistance to standard antimicrobial approaches, alternative strategies employing photocatalysis or control of biofilm formation by modifying surfaces, have been proposed. Colony forming units (cfu) counting and live/dead staining, two classic techniques used for biofilm quantification, are detailed in this work. Both methods assess cell viability, a key factor to analyze the microbial susceptibility to given treatment, then, they represent a good approach for evaluation of an antibiofilm strategy.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138270
Pezzoni, Magdalena; Pizarro, Ramón Augusto; Costa, Cristina Susana; Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining; Bio-protocol; Bio-Protocol; 10; 18; 9-2020; 1-12
2331-8325
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138270
identifier_str_mv Pezzoni, Magdalena; Pizarro, Ramón Augusto; Costa, Cristina Susana; Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining; Bio-protocol; Bio-Protocol; 10; 18; 9-2020; 1-12
2331-8325
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e3762
info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.3762
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-protocol
publisher.none.fl_str_mv Bio-protocol
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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