Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors

Autores
Ramos Vernieri, Alberto Nicolás; Peral, M.C.; Valdéz, Juan Carlos
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We study the differences between Pseudomonas aeruginosa from an infected wound (clinical strain) and a colony isolated from it. We assessed the in vitro inhibition of these P. aeruginosa biofilms by DNase and filtrate of Lactobacillus plantarum cultures (acid = AF and neutralize = NF) with crystal violet technique. Inhibition by AF was greatest than DNase for clinical and isolated strain (p<0.001) and greatest than NF for clinical (p<0.05) and isolated strain (p<0.001).Using a burn model in mice, we compared the infection producing by clinical and isolated strains in planktonic and biofilm form. Deaths were quantified and the infection was assessed by determining CFU/g of tissue in the lesion, spleen and liver.The infections with planktonic bacteria tended to become systemic and more deadly than biofilm infections. All infected wounds required the same healing period (30 days). These findings were independent of the origin of the bacteria (clinical or colony isolated strain). © 2008 Elsevier Ltd.
Fil: Ramos Vernieri, Alberto Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; Argentina
Fil: Peral, M.C.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Valdéz, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; Argentina
Materia
Bacterial Interference
Biofilm Inhibition
Burns
Lactobacillus Plantarum
Pseudomonas Aeruginosa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62740
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitorsRamos Vernieri, Alberto NicolásPeral, M.C.Valdéz, Juan CarlosBacterial InterferenceBiofilm InhibitionBurnsLactobacillus PlantarumPseudomonas Aeruginosahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We study the differences between Pseudomonas aeruginosa from an infected wound (clinical strain) and a colony isolated from it. We assessed the in vitro inhibition of these P. aeruginosa biofilms by DNase and filtrate of Lactobacillus plantarum cultures (acid = AF and neutralize = NF) with crystal violet technique. Inhibition by AF was greatest than DNase for clinical and isolated strain (p<0.001) and greatest than NF for clinical (p<0.05) and isolated strain (p<0.001).Using a burn model in mice, we compared the infection producing by clinical and isolated strains in planktonic and biofilm form. Deaths were quantified and the infection was assessed by determining CFU/g of tissue in the lesion, spleen and liver.The infections with planktonic bacteria tended to become systemic and more deadly than biofilm infections. All infected wounds required the same healing period (30 days). These findings were independent of the origin of the bacteria (clinical or colony isolated strain). © 2008 Elsevier Ltd.Fil: Ramos Vernieri, Alberto Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; ArgentinaFil: Peral, M.C.. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Valdéz, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; ArgentinaElsevier2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62740Ramos Vernieri, Alberto Nicolás; Peral, M.C.; Valdéz, Juan Carlos; Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 33; 3; 5-2010; 267-2750147-9571CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0147957108000611info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2008.10.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:44Zoai:ri.conicet.gov.ar:11336/62740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:44.899CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
title Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
spellingShingle Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
Ramos Vernieri, Alberto Nicolás
Bacterial Interference
Biofilm Inhibition
Burns
Lactobacillus Plantarum
Pseudomonas Aeruginosa
title_short Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
title_full Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
title_fullStr Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
title_full_unstemmed Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
title_sort Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors
dc.creator.none.fl_str_mv Ramos Vernieri, Alberto Nicolás
Peral, M.C.
Valdéz, Juan Carlos
author Ramos Vernieri, Alberto Nicolás
author_facet Ramos Vernieri, Alberto Nicolás
Peral, M.C.
Valdéz, Juan Carlos
author_role author
author2 Peral, M.C.
Valdéz, Juan Carlos
author2_role author
author
dc.subject.none.fl_str_mv Bacterial Interference
Biofilm Inhibition
Burns
Lactobacillus Plantarum
Pseudomonas Aeruginosa
topic Bacterial Interference
Biofilm Inhibition
Burns
Lactobacillus Plantarum
Pseudomonas Aeruginosa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We study the differences between Pseudomonas aeruginosa from an infected wound (clinical strain) and a colony isolated from it. We assessed the in vitro inhibition of these P. aeruginosa biofilms by DNase and filtrate of Lactobacillus plantarum cultures (acid = AF and neutralize = NF) with crystal violet technique. Inhibition by AF was greatest than DNase for clinical and isolated strain (p<0.001) and greatest than NF for clinical (p<0.05) and isolated strain (p<0.001).Using a burn model in mice, we compared the infection producing by clinical and isolated strains in planktonic and biofilm form. Deaths were quantified and the infection was assessed by determining CFU/g of tissue in the lesion, spleen and liver.The infections with planktonic bacteria tended to become systemic and more deadly than biofilm infections. All infected wounds required the same healing period (30 days). These findings were independent of the origin of the bacteria (clinical or colony isolated strain). © 2008 Elsevier Ltd.
Fil: Ramos Vernieri, Alberto Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; Argentina
Fil: Peral, M.C.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Valdéz, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; Argentina
description We study the differences between Pseudomonas aeruginosa from an infected wound (clinical strain) and a colony isolated from it. We assessed the in vitro inhibition of these P. aeruginosa biofilms by DNase and filtrate of Lactobacillus plantarum cultures (acid = AF and neutralize = NF) with crystal violet technique. Inhibition by AF was greatest than DNase for clinical and isolated strain (p<0.001) and greatest than NF for clinical (p<0.05) and isolated strain (p<0.001).Using a burn model in mice, we compared the infection producing by clinical and isolated strains in planktonic and biofilm form. Deaths were quantified and the infection was assessed by determining CFU/g of tissue in the lesion, spleen and liver.The infections with planktonic bacteria tended to become systemic and more deadly than biofilm infections. All infected wounds required the same healing period (30 days). These findings were independent of the origin of the bacteria (clinical or colony isolated strain). © 2008 Elsevier Ltd.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62740
Ramos Vernieri, Alberto Nicolás; Peral, M.C.; Valdéz, Juan Carlos; Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 33; 3; 5-2010; 267-275
0147-9571
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62740
identifier_str_mv Ramos Vernieri, Alberto Nicolás; Peral, M.C.; Valdéz, Juan Carlos; Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 33; 3; 5-2010; 267-275
0147-9571
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0147957108000611
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2008.10.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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