Bacterial nanocellulose production from naphthalene

Autores
Marín, Patricia; Martirani Von Abercron, Sophie Marie; Urbina, Leire; Pacheco Sánchez, Daniel; Castañeda Cataña, Mayra Alejandra; Retegi, Aloña; Eceiza, Arantxa; Marqués, Silvia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH-polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characterize a new Xanthobacteraceae strain, Starkeya sp. strain N1B, previously isolated during enrichment under microaerophilic conditions, which is capable of using naphthalene crystals as the sole carbon source. The strain produced a structured biofilm when grown on naphthalene crystals, which had the shape of a half-sphere organized over the crystal. Scanning electron microscopy (SEM) and GC-MS analysis indicated that the biofilm was essentially made of cellulose, composed of several micron-long nanofibrils of 60 nm diameter. A cellulosic biofilm was also formed when the cells grew with glucose as the carbon source. Fourier transformed infrared spectroscopy (FTIR) confirmed that the polymer was type I cellulose in both cases, although the crystallinity of the material greatly depended on the carbon source used for growth. Using genome mining and mutant analysis, we identified the genetic complements required for the transformation of naphthalene into cellulose, which seemed to have been successively acquired through horizontal gene transfer. The capacity to develop the biofilm around the crystal was found to be dispensable for growth when naphthalene was used as the carbon source, suggesting that the function of this structure is more intricate than initially thought. This is the first example of the use of toxic aromatic hydrocarbons as the carbon source for bacterial cellulose production. Application of this capacity would allow the remediation of a PAH into such a value-added polymer with multiple biotechnological usages.
Fil: Marín, Patricia. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Martirani Von Abercron, Sophie Marie. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Urbina, Leire. Universidad del País Vasco; España
Fil: Pacheco Sánchez, Daniel. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Castañeda Cataña, Mayra Alejandra. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Retegi, Aloña. Universidad del País Vasco; España
Fil: Eceiza, Arantxa. Universidad del País Vasco; España
Fil: Marqués, Silvia. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Materia
Xanthobacteraceae strain
Polycyclic aromatic compounds (PAHs)
cellulose
Starkeya sp
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180809
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180809
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bacterial nanocellulose production from naphthaleneMarín, PatriciaMartirani Von Abercron, Sophie MarieUrbina, LeirePacheco Sánchez, DanielCastañeda Cataña, Mayra AlejandraRetegi, AloñaEceiza, ArantxaMarqués, SilviaXanthobacteraceae strainPolycyclic aromatic compounds (PAHs)celluloseStarkeya sphttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH-polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characterize a new Xanthobacteraceae strain, Starkeya sp. strain N1B, previously isolated during enrichment under microaerophilic conditions, which is capable of using naphthalene crystals as the sole carbon source. The strain produced a structured biofilm when grown on naphthalene crystals, which had the shape of a half-sphere organized over the crystal. Scanning electron microscopy (SEM) and GC-MS analysis indicated that the biofilm was essentially made of cellulose, composed of several micron-long nanofibrils of 60 nm diameter. A cellulosic biofilm was also formed when the cells grew with glucose as the carbon source. Fourier transformed infrared spectroscopy (FTIR) confirmed that the polymer was type I cellulose in both cases, although the crystallinity of the material greatly depended on the carbon source used for growth. Using genome mining and mutant analysis, we identified the genetic complements required for the transformation of naphthalene into cellulose, which seemed to have been successively acquired through horizontal gene transfer. The capacity to develop the biofilm around the crystal was found to be dispensable for growth when naphthalene was used as the carbon source, suggesting that the function of this structure is more intricate than initially thought. This is the first example of the use of toxic aromatic hydrocarbons as the carbon source for bacterial cellulose production. Application of this capacity would allow the remediation of a PAH into such a value-added polymer with multiple biotechnological usages.Fil: Marín, Patricia. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Martirani Von Abercron, Sophie Marie. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Urbina, Leire. Universidad del País Vasco; EspañaFil: Pacheco Sánchez, Daniel. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Castañeda Cataña, Mayra Alejandra. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Retegi, Aloña. Universidad del País Vasco; EspañaFil: Eceiza, Arantxa. Universidad del País Vasco; EspañaFil: Marqués, Silvia. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaJohn Wiley & Sons Ltd2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180809Marín, Patricia; Martirani Von Abercron, Sophie Marie; Urbina, Leire; Pacheco Sánchez, Daniel; Castañeda Cataña, Mayra Alejandra; et al.; Bacterial nanocellulose production from naphthalene; John Wiley & Sons Ltd; Microbial Biotechnology; 12; 4; 7-2019; 662-6761751-79071751-7915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1751-7915.13399info:eu-repo/semantics/altIdentifier/doi/10.1111/1751-7915.13399info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:38Zoai:ri.conicet.gov.ar:11336/180809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:39.03CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacterial nanocellulose production from naphthalene
title Bacterial nanocellulose production from naphthalene
spellingShingle Bacterial nanocellulose production from naphthalene
Marín, Patricia
Xanthobacteraceae strain
Polycyclic aromatic compounds (PAHs)
cellulose
Starkeya sp
title_short Bacterial nanocellulose production from naphthalene
title_full Bacterial nanocellulose production from naphthalene
title_fullStr Bacterial nanocellulose production from naphthalene
title_full_unstemmed Bacterial nanocellulose production from naphthalene
title_sort Bacterial nanocellulose production from naphthalene
dc.creator.none.fl_str_mv Marín, Patricia
Martirani Von Abercron, Sophie Marie
Urbina, Leire
Pacheco Sánchez, Daniel
Castañeda Cataña, Mayra Alejandra
Retegi, Aloña
Eceiza, Arantxa
Marqués, Silvia
author Marín, Patricia
author_facet Marín, Patricia
Martirani Von Abercron, Sophie Marie
Urbina, Leire
Pacheco Sánchez, Daniel
Castañeda Cataña, Mayra Alejandra
Retegi, Aloña
Eceiza, Arantxa
Marqués, Silvia
author_role author
author2 Martirani Von Abercron, Sophie Marie
Urbina, Leire
Pacheco Sánchez, Daniel
Castañeda Cataña, Mayra Alejandra
Retegi, Aloña
Eceiza, Arantxa
Marqués, Silvia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Xanthobacteraceae strain
Polycyclic aromatic compounds (PAHs)
cellulose
Starkeya sp
topic Xanthobacteraceae strain
Polycyclic aromatic compounds (PAHs)
cellulose
Starkeya sp
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH-polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characterize a new Xanthobacteraceae strain, Starkeya sp. strain N1B, previously isolated during enrichment under microaerophilic conditions, which is capable of using naphthalene crystals as the sole carbon source. The strain produced a structured biofilm when grown on naphthalene crystals, which had the shape of a half-sphere organized over the crystal. Scanning electron microscopy (SEM) and GC-MS analysis indicated that the biofilm was essentially made of cellulose, composed of several micron-long nanofibrils of 60 nm diameter. A cellulosic biofilm was also formed when the cells grew with glucose as the carbon source. Fourier transformed infrared spectroscopy (FTIR) confirmed that the polymer was type I cellulose in both cases, although the crystallinity of the material greatly depended on the carbon source used for growth. Using genome mining and mutant analysis, we identified the genetic complements required for the transformation of naphthalene into cellulose, which seemed to have been successively acquired through horizontal gene transfer. The capacity to develop the biofilm around the crystal was found to be dispensable for growth when naphthalene was used as the carbon source, suggesting that the function of this structure is more intricate than initially thought. This is the first example of the use of toxic aromatic hydrocarbons as the carbon source for bacterial cellulose production. Application of this capacity would allow the remediation of a PAH into such a value-added polymer with multiple biotechnological usages.
Fil: Marín, Patricia. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Martirani Von Abercron, Sophie Marie. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Urbina, Leire. Universidad del País Vasco; España
Fil: Pacheco Sánchez, Daniel. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Castañeda Cataña, Mayra Alejandra. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Retegi, Aloña. Universidad del País Vasco; España
Fil: Eceiza, Arantxa. Universidad del País Vasco; España
Fil: Marqués, Silvia. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
description Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH-polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characterize a new Xanthobacteraceae strain, Starkeya sp. strain N1B, previously isolated during enrichment under microaerophilic conditions, which is capable of using naphthalene crystals as the sole carbon source. The strain produced a structured biofilm when grown on naphthalene crystals, which had the shape of a half-sphere organized over the crystal. Scanning electron microscopy (SEM) and GC-MS analysis indicated that the biofilm was essentially made of cellulose, composed of several micron-long nanofibrils of 60 nm diameter. A cellulosic biofilm was also formed when the cells grew with glucose as the carbon source. Fourier transformed infrared spectroscopy (FTIR) confirmed that the polymer was type I cellulose in both cases, although the crystallinity of the material greatly depended on the carbon source used for growth. Using genome mining and mutant analysis, we identified the genetic complements required for the transformation of naphthalene into cellulose, which seemed to have been successively acquired through horizontal gene transfer. The capacity to develop the biofilm around the crystal was found to be dispensable for growth when naphthalene was used as the carbon source, suggesting that the function of this structure is more intricate than initially thought. This is the first example of the use of toxic aromatic hydrocarbons as the carbon source for bacterial cellulose production. Application of this capacity would allow the remediation of a PAH into such a value-added polymer with multiple biotechnological usages.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180809
Marín, Patricia; Martirani Von Abercron, Sophie Marie; Urbina, Leire; Pacheco Sánchez, Daniel; Castañeda Cataña, Mayra Alejandra; et al.; Bacterial nanocellulose production from naphthalene; John Wiley & Sons Ltd; Microbial Biotechnology; 12; 4; 7-2019; 662-676
1751-7907
1751-7915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180809
identifier_str_mv Marín, Patricia; Martirani Von Abercron, Sophie Marie; Urbina, Leire; Pacheco Sánchez, Daniel; Castañeda Cataña, Mayra Alejandra; et al.; Bacterial nanocellulose production from naphthalene; John Wiley & Sons Ltd; Microbial Biotechnology; 12; 4; 7-2019; 662-676
1751-7907
1751-7915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1751-7915.13399
info:eu-repo/semantics/altIdentifier/doi/10.1111/1751-7915.13399
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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