The role of cytoskeleton networks on lipid-mediated delivery of DNA

Autores
Coppola, Stefano; Cardarelli, Francesco; Pozzi, Daniela; Estrada, Laura Cecilia; Digman, Michelle; Gratton, Enrico; Bifone, Angelo; Marianecci, Carlotta; Caracciolo, Giulio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Lipid-mediated delivery of DNA is hindered by extracellular and intracellular barriers that significantly reduce the transfection efficiency of synthetic nonviral vectors. Results: In this study we investigated the role of the actin and microtubule networks on the uptake and cytoplasmic transport of multicomponent cationic liposome–DNA complexes in CHO-K1 live cells by means of confocal laser scanning microscopy and 3D single particle tracking. Treatment with actin (latrunculin B)- and microtubule-disrupting (nocodazole) reagents indicated that intracellular trafficking of complexes predominantly involves microtubule-dependent active transport. We found that the actin network has a major effect on the initial uptake of complexes, while the microtubule network is mainly responsible for the subsequent active transportation to the lysosomes. Conclusion: Collectively, a strategy to improve the efficiency of lipid gene vectors can be formulated. We could find a lipid formulation that allows the nanoparticles to avoid the microtubule pathway to lysosomes.
Fil: Coppola, Stefano. Sapienza University of Rome. Dipartimento di Medicina Molecolare; Italia
Fil: Cardarelli, Francesco. Istituto Italiano di Tecnologia. Center for Nanotechnology Innovation at NEST; Italia
Fil: Pozzi, Daniela. Sapienza University of Rome. Dipartimento di Medicina Molecolare; Italia
Fil: Estrada, Laura Cecilia. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física. Laboratorio de Electrónica Cuántica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina
Fil: Digman, Michelle. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados Unidos
Fil: Gratton, Enrico. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados Unidos
Fil: Bifone, Angelo . Istituto Italiano di Tecnologia. Center for Nanotechnology Innovation at NEST; Italia
Fil: Marianecci, Carlotta. Sapienza University of Rome. Dipartimento di Chimica e Tecnologie del Farmaco; Italia
Fil: Caracciolo, Giulio. Sapienza University of Rome. Dipartimento di Medicina Molecolare; Italia
Materia
Drug Delivery
Single Particle Tracking
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2770
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2770
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The role of cytoskeleton networks on lipid-mediated delivery of DNACoppola, StefanoCardarelli, FrancescoPozzi, DanielaEstrada, Laura CeciliaDigman, MichelleGratton, EnricoBifone, Angelo Marianecci, CarlottaCaracciolo, GiulioDrug DeliverySingle Particle Trackinghttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Lipid-mediated delivery of DNA is hindered by extracellular and intracellular barriers that significantly reduce the transfection efficiency of synthetic nonviral vectors. Results: In this study we investigated the role of the actin and microtubule networks on the uptake and cytoplasmic transport of multicomponent cationic liposome–DNA complexes in CHO-K1 live cells by means of confocal laser scanning microscopy and 3D single particle tracking. Treatment with actin (latrunculin B)- and microtubule-disrupting (nocodazole) reagents indicated that intracellular trafficking of complexes predominantly involves microtubule-dependent active transport. We found that the actin network has a major effect on the initial uptake of complexes, while the microtubule network is mainly responsible for the subsequent active transportation to the lysosomes. Conclusion: Collectively, a strategy to improve the efficiency of lipid gene vectors can be formulated. We could find a lipid formulation that allows the nanoparticles to avoid the microtubule pathway to lysosomes.Fil: Coppola, Stefano. Sapienza University of Rome. Dipartimento di Medicina Molecolare; ItaliaFil: Cardarelli, Francesco. Istituto Italiano di Tecnologia. Center for Nanotechnology Innovation at NEST; ItaliaFil: Pozzi, Daniela. Sapienza University of Rome. Dipartimento di Medicina Molecolare; ItaliaFil: Estrada, Laura Cecilia. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física. Laboratorio de Electrónica Cuántica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; ArgentinaFil: Digman, Michelle. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados UnidosFil: Gratton, Enrico. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados UnidosFil: Bifone, Angelo . Istituto Italiano di Tecnologia. Center for Nanotechnology Innovation at NEST; ItaliaFil: Marianecci, Carlotta. Sapienza University of Rome. Dipartimento di Chimica e Tecnologie del Farmaco; ItaliaFil: Caracciolo, Giulio. Sapienza University of Rome. Dipartimento di Medicina Molecolare; ItaliaFuture Science2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2770Coppola, Stefano; Cardarelli, Francesco; Pozzi, Daniela; Estrada, Laura Cecilia; Digman, Michelle; et al.; The role of cytoskeleton networks on lipid-mediated delivery of DNA; Future Science; Therapeutic Delivery; 4; 2; 2-2013; 191-2022041-5990enginfo:eu-repo/semantics/altIdentifier/url/http://www.future-science.com/doi/abs/10.4155/tde.12.151?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&info:eu-repo/semantics/altIdentifier/doi/10.4155/tde.12.151info:eu-repo/semantics/altIdentifier/issn/2041-5990info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771858/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:50Zoai:ri.conicet.gov.ar:11336/2770instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:51.219CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The role of cytoskeleton networks on lipid-mediated delivery of DNA
title The role of cytoskeleton networks on lipid-mediated delivery of DNA
spellingShingle The role of cytoskeleton networks on lipid-mediated delivery of DNA
Coppola, Stefano
Drug Delivery
Single Particle Tracking
title_short The role of cytoskeleton networks on lipid-mediated delivery of DNA
title_full The role of cytoskeleton networks on lipid-mediated delivery of DNA
title_fullStr The role of cytoskeleton networks on lipid-mediated delivery of DNA
title_full_unstemmed The role of cytoskeleton networks on lipid-mediated delivery of DNA
title_sort The role of cytoskeleton networks on lipid-mediated delivery of DNA
dc.creator.none.fl_str_mv Coppola, Stefano
Cardarelli, Francesco
Pozzi, Daniela
Estrada, Laura Cecilia
Digman, Michelle
Gratton, Enrico
Bifone, Angelo
Marianecci, Carlotta
Caracciolo, Giulio
author Coppola, Stefano
author_facet Coppola, Stefano
Cardarelli, Francesco
Pozzi, Daniela
Estrada, Laura Cecilia
Digman, Michelle
Gratton, Enrico
Bifone, Angelo
Marianecci, Carlotta
Caracciolo, Giulio
author_role author
author2 Cardarelli, Francesco
Pozzi, Daniela
Estrada, Laura Cecilia
Digman, Michelle
Gratton, Enrico
Bifone, Angelo
Marianecci, Carlotta
Caracciolo, Giulio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Drug Delivery
Single Particle Tracking
topic Drug Delivery
Single Particle Tracking
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Lipid-mediated delivery of DNA is hindered by extracellular and intracellular barriers that significantly reduce the transfection efficiency of synthetic nonviral vectors. Results: In this study we investigated the role of the actin and microtubule networks on the uptake and cytoplasmic transport of multicomponent cationic liposome–DNA complexes in CHO-K1 live cells by means of confocal laser scanning microscopy and 3D single particle tracking. Treatment with actin (latrunculin B)- and microtubule-disrupting (nocodazole) reagents indicated that intracellular trafficking of complexes predominantly involves microtubule-dependent active transport. We found that the actin network has a major effect on the initial uptake of complexes, while the microtubule network is mainly responsible for the subsequent active transportation to the lysosomes. Conclusion: Collectively, a strategy to improve the efficiency of lipid gene vectors can be formulated. We could find a lipid formulation that allows the nanoparticles to avoid the microtubule pathway to lysosomes.
Fil: Coppola, Stefano. Sapienza University of Rome. Dipartimento di Medicina Molecolare; Italia
Fil: Cardarelli, Francesco. Istituto Italiano di Tecnologia. Center for Nanotechnology Innovation at NEST; Italia
Fil: Pozzi, Daniela. Sapienza University of Rome. Dipartimento di Medicina Molecolare; Italia
Fil: Estrada, Laura Cecilia. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física. Laboratorio de Electrónica Cuántica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina
Fil: Digman, Michelle. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados Unidos
Fil: Gratton, Enrico. University of California. Department of Biomedical Engineering. Laboratory for Fluorescence Dynamics; Estados Unidos
Fil: Bifone, Angelo . Istituto Italiano di Tecnologia. Center for Nanotechnology Innovation at NEST; Italia
Fil: Marianecci, Carlotta. Sapienza University of Rome. Dipartimento di Chimica e Tecnologie del Farmaco; Italia
Fil: Caracciolo, Giulio. Sapienza University of Rome. Dipartimento di Medicina Molecolare; Italia
description Background: Lipid-mediated delivery of DNA is hindered by extracellular and intracellular barriers that significantly reduce the transfection efficiency of synthetic nonviral vectors. Results: In this study we investigated the role of the actin and microtubule networks on the uptake and cytoplasmic transport of multicomponent cationic liposome–DNA complexes in CHO-K1 live cells by means of confocal laser scanning microscopy and 3D single particle tracking. Treatment with actin (latrunculin B)- and microtubule-disrupting (nocodazole) reagents indicated that intracellular trafficking of complexes predominantly involves microtubule-dependent active transport. We found that the actin network has a major effect on the initial uptake of complexes, while the microtubule network is mainly responsible for the subsequent active transportation to the lysosomes. Conclusion: Collectively, a strategy to improve the efficiency of lipid gene vectors can be formulated. We could find a lipid formulation that allows the nanoparticles to avoid the microtubule pathway to lysosomes.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2770
Coppola, Stefano; Cardarelli, Francesco; Pozzi, Daniela; Estrada, Laura Cecilia; Digman, Michelle; et al.; The role of cytoskeleton networks on lipid-mediated delivery of DNA; Future Science; Therapeutic Delivery; 4; 2; 2-2013; 191-202
2041-5990
url http://hdl.handle.net/11336/2770
identifier_str_mv Coppola, Stefano; Cardarelli, Francesco; Pozzi, Daniela; Estrada, Laura Cecilia; Digman, Michelle; et al.; The role of cytoskeleton networks on lipid-mediated delivery of DNA; Future Science; Therapeutic Delivery; 4; 2; 2-2013; 191-202
2041-5990
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.future-science.com/doi/abs/10.4155/tde.12.151?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&
info:eu-repo/semantics/altIdentifier/doi/10.4155/tde.12.151
info:eu-repo/semantics/altIdentifier/issn/2041-5990
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771858/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Future Science
publisher.none.fl_str_mv Future Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613566112464896
score 13.070432

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