Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models

Autores
Romussi, Stéfano; Andersen, Natalia Denise; Ibarguren, Sofia; Rayes, Diego Hernán; De Rosa, María José
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Due to the escalating life expectancy, the prevalence of age-related disorders like neurodegenerative diseases (ND) has surged. Oxidative stress (OS) has emerged as a key accelerator in ND progression. In Parkinson’s disease (PD), for instance, compromised free radical scavenging capacity has been linked to worsened α-synuclein ag- gregation and proteotoxic damage. Geraniol (GER), a plant-derived essential oil, has recognized antioxidant properties. Considering that OS contributes to ND progression, compounds with antioxidant activity have been postulated as potential therapeutic agents. Leveraging the suitability of Caenorhabditis elegans as a model organism in biomedical research due to its genetic homology with mammals, including cytoprotective proteins, we aim to assess GER’s biological efficacy in a C. elegans PD model and delve into the underlying molecular mechanisms. Our results first confirmed the in vivo antiox- idant activity of GER. We cultured wild-type animals with GER and then exposed them to Juglone (oxidative agent). Locomotion was employed as a survival proxy using the Worm MicroTracker device. Strikingly, GER exhibited a significant increase in animal survival under OS conditions (P=<0.001). To unravel the precise mechanism driving GER’s protective effects, we analyzed null mutants within key molecular pathways associated with OS response. Intriguing- ly, our preliminary findings showed that either DAF16/FOXO, HSF1 or SKN1/NRF2 are involved in mediating GER’s protective effect. Given the link between OS and PD, we also evaluated the GER´s impact within a C. elegans PD model. We found that GER improves the impaired-locomotion phenotype of this model (P = 0,011). So far, these results indicate a potential antiproteotoxic effect of GER in C. elegans PD models. To comprehensively dissect GER’s effects, we propose an integrative approach involving genetic analyses, advanced microscopy techniques, and behavioral assessments.
Fil: Romussi, Stéfano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Ibarguren, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: De Rosa, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Asociación Argentina de Farmacología Experimental
Sociedad Argentina De Investigación Clínica
Sociedad Argentina De Biología
Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio
Materia
INVERTEBRADOS
C. ELEGANS
PROTEOSTASIS
OXIDATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/250156
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease modelsRomussi, StéfanoAndersen, Natalia DeniseIbarguren, SofiaRayes, Diego HernánDe Rosa, María JoséINVERTEBRADOSC. ELEGANSPROTEOSTASISOXIDATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Due to the escalating life expectancy, the prevalence of age-related disorders like neurodegenerative diseases (ND) has surged. Oxidative stress (OS) has emerged as a key accelerator in ND progression. In Parkinson’s disease (PD), for instance, compromised free radical scavenging capacity has been linked to worsened α-synuclein ag- gregation and proteotoxic damage. Geraniol (GER), a plant-derived essential oil, has recognized antioxidant properties. Considering that OS contributes to ND progression, compounds with antioxidant activity have been postulated as potential therapeutic agents. Leveraging the suitability of Caenorhabditis elegans as a model organism in biomedical research due to its genetic homology with mammals, including cytoprotective proteins, we aim to assess GER’s biological efficacy in a C. elegans PD model and delve into the underlying molecular mechanisms. Our results first confirmed the in vivo antiox- idant activity of GER. We cultured wild-type animals with GER and then exposed them to Juglone (oxidative agent). Locomotion was employed as a survival proxy using the Worm MicroTracker device. Strikingly, GER exhibited a significant increase in animal survival under OS conditions (P=<0.001). To unravel the precise mechanism driving GER’s protective effects, we analyzed null mutants within key molecular pathways associated with OS response. Intriguing- ly, our preliminary findings showed that either DAF16/FOXO, HSF1 or SKN1/NRF2 are involved in mediating GER’s protective effect. Given the link between OS and PD, we also evaluated the GER´s impact within a C. elegans PD model. We found that GER improves the impaired-locomotion phenotype of this model (P = 0,011). So far, these results indicate a potential antiproteotoxic effect of GER in C. elegans PD models. To comprehensively dissect GER’s effects, we propose an integrative approach involving genetic analyses, advanced microscopy techniques, and behavioral assessments.Fil: Romussi, Stéfano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Ibarguren, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: De Rosa, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaAsociación Argentina de Farmacología ExperimentalSociedad Argentina De Investigación ClínicaSociedad Argentina De BiologíaAsociación Argentina De Ciencia Y Tecnología De Animales De LaboratorioFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/250156Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 224-2240025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:13Zoai:ri.conicet.gov.ar:11336/250156instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:13.436CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
title Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
spellingShingle Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
Romussi, Stéfano
INVERTEBRADOS
C. ELEGANS
PROTEOSTASIS
OXIDATIVE STRESS
title_short Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
title_full Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
title_fullStr Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
title_full_unstemmed Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
title_sort Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models
dc.creator.none.fl_str_mv Romussi, Stéfano
Andersen, Natalia Denise
Ibarguren, Sofia
Rayes, Diego Hernán
De Rosa, María José
author Romussi, Stéfano
author_facet Romussi, Stéfano
Andersen, Natalia Denise
Ibarguren, Sofia
Rayes, Diego Hernán
De Rosa, María José
author_role author
author2 Andersen, Natalia Denise
Ibarguren, Sofia
Rayes, Diego Hernán
De Rosa, María José
author2_role author
author
author
author
dc.subject.none.fl_str_mv INVERTEBRADOS
C. ELEGANS
PROTEOSTASIS
OXIDATIVE STRESS
topic INVERTEBRADOS
C. ELEGANS
PROTEOSTASIS
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Due to the escalating life expectancy, the prevalence of age-related disorders like neurodegenerative diseases (ND) has surged. Oxidative stress (OS) has emerged as a key accelerator in ND progression. In Parkinson’s disease (PD), for instance, compromised free radical scavenging capacity has been linked to worsened α-synuclein ag- gregation and proteotoxic damage. Geraniol (GER), a plant-derived essential oil, has recognized antioxidant properties. Considering that OS contributes to ND progression, compounds with antioxidant activity have been postulated as potential therapeutic agents. Leveraging the suitability of Caenorhabditis elegans as a model organism in biomedical research due to its genetic homology with mammals, including cytoprotective proteins, we aim to assess GER’s biological efficacy in a C. elegans PD model and delve into the underlying molecular mechanisms. Our results first confirmed the in vivo antiox- idant activity of GER. We cultured wild-type animals with GER and then exposed them to Juglone (oxidative agent). Locomotion was employed as a survival proxy using the Worm MicroTracker device. Strikingly, GER exhibited a significant increase in animal survival under OS conditions (P=<0.001). To unravel the precise mechanism driving GER’s protective effects, we analyzed null mutants within key molecular pathways associated with OS response. Intriguing- ly, our preliminary findings showed that either DAF16/FOXO, HSF1 or SKN1/NRF2 are involved in mediating GER’s protective effect. Given the link between OS and PD, we also evaluated the GER´s impact within a C. elegans PD model. We found that GER improves the impaired-locomotion phenotype of this model (P = 0,011). So far, these results indicate a potential antiproteotoxic effect of GER in C. elegans PD models. To comprehensively dissect GER’s effects, we propose an integrative approach involving genetic analyses, advanced microscopy techniques, and behavioral assessments.
Fil: Romussi, Stéfano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Ibarguren, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: De Rosa, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Asociación Argentina de Farmacología Experimental
Sociedad Argentina De Investigación Clínica
Sociedad Argentina De Biología
Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio
description Due to the escalating life expectancy, the prevalence of age-related disorders like neurodegenerative diseases (ND) has surged. Oxidative stress (OS) has emerged as a key accelerator in ND progression. In Parkinson’s disease (PD), for instance, compromised free radical scavenging capacity has been linked to worsened α-synuclein ag- gregation and proteotoxic damage. Geraniol (GER), a plant-derived essential oil, has recognized antioxidant properties. Considering that OS contributes to ND progression, compounds with antioxidant activity have been postulated as potential therapeutic agents. Leveraging the suitability of Caenorhabditis elegans as a model organism in biomedical research due to its genetic homology with mammals, including cytoprotective proteins, we aim to assess GER’s biological efficacy in a C. elegans PD model and delve into the underlying molecular mechanisms. Our results first confirmed the in vivo antiox- idant activity of GER. We cultured wild-type animals with GER and then exposed them to Juglone (oxidative agent). Locomotion was employed as a survival proxy using the Worm MicroTracker device. Strikingly, GER exhibited a significant increase in animal survival under OS conditions (P=<0.001). To unravel the precise mechanism driving GER’s protective effects, we analyzed null mutants within key molecular pathways associated with OS response. Intriguing- ly, our preliminary findings showed that either DAF16/FOXO, HSF1 or SKN1/NRF2 are involved in mediating GER’s protective effect. Given the link between OS and PD, we also evaluated the GER´s impact within a C. elegans PD model. We found that GER improves the impaired-locomotion phenotype of this model (P = 0,011). So far, these results indicate a potential antiproteotoxic effect of GER in C. elegans PD models. To comprehensively dissect GER’s effects, we propose an integrative approach involving genetic analyses, advanced microscopy techniques, and behavioral assessments.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/250156
Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 224-224
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/250156
identifier_str_mv Geraniol protects against oxidative stress and proteotoxicity in C. elegans Parkinson's disease models; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 224-224
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/
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dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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