Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
- Autores
- Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; Albonico, Marco; Degani, Monica; Tais, Stefano; Angheben, Andrea; Requena Mendez, Ana; Muñoz, Jose; Nutman, Thomas; Bisoffi, Zeno
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cure
Fil: Buonfrate, Dora. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
Fil: Sequi, Marco. Mario Negri Institute for Pharmacological Research. Department of Public Health; Italia
Fil: Mejia, Rojelio. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Cimino, Ruben Oscar. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Albonico, Marco. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Degani, Monica. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Tais, Stefano. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Angheben, Andrea. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
Fil: Requena Mendez, Ana. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España
Fil: Muñoz, Jose. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España
Fil: Nutman, Thomas. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Bisoffi, Zeno. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia - Materia
-
STRONGYLOIDES STERCORALIS
SEROLOGÍA
ELISA
GEOHELMINTOS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/6988
Ver los metadatos del registro completo
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Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis InfectionBuonfrate, DoraSequi, MarcoMejia, RojelioCimino, Ruben OscarKrolewiecki, Alejandro JavierAlbonico, MarcoDegani, MonicaTais, StefanoAngheben, AndreaRequena Mendez, AnaMuñoz, JoseNutman, ThomasBisoffi, ZenoSTRONGYLOIDES STERCORALISSEROLOGÍAELISAGEOHELMINTOShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cureFil: Buonfrate, Dora. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; ItaliaFil: Sequi, Marco. Mario Negri Institute for Pharmacological Research. Department of Public Health; ItaliaFil: Mejia, Rojelio. Public Health Service. National Institute Of Health; Estados UnidosFil: Cimino, Ruben Oscar. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Albonico, Marco. Sacro Cuore Hospital. Center for Tropical Diseases; ItaliaFil: Degani, Monica. Sacro Cuore Hospital. Center for Tropical Diseases; ItaliaFil: Tais, Stefano. Sacro Cuore Hospital. Center for Tropical Diseases; ItaliaFil: Angheben, Andrea. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; ItaliaFil: Requena Mendez, Ana. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; EspañaFil: Muñoz, Jose. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; EspañaFil: Nutman, Thomas. Public Health Service. National Institute Of Health; Estados UnidosFil: Bisoffi, Zeno. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; ItaliaPublic Library Of Science2015-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6988Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; et al.; Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection; Public Library Of Science; Neglected Tropical Diseases; 9; 2; 10-2-2015; e0003491-e00034911935-2735enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003491info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003491info:eu-repo/semantics/altIdentifier/pmid/PMC4323101info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323101/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:26Zoai:ri.conicet.gov.ar:11336/6988instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:26.926CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
title |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
spellingShingle |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection Buonfrate, Dora STRONGYLOIDES STERCORALIS SEROLOGÍA ELISA GEOHELMINTOS |
title_short |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
title_full |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
title_fullStr |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
title_full_unstemmed |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
title_sort |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
dc.creator.none.fl_str_mv |
Buonfrate, Dora Sequi, Marco Mejia, Rojelio Cimino, Ruben Oscar Krolewiecki, Alejandro Javier Albonico, Marco Degani, Monica Tais, Stefano Angheben, Andrea Requena Mendez, Ana Muñoz, Jose Nutman, Thomas Bisoffi, Zeno |
author |
Buonfrate, Dora |
author_facet |
Buonfrate, Dora Sequi, Marco Mejia, Rojelio Cimino, Ruben Oscar Krolewiecki, Alejandro Javier Albonico, Marco Degani, Monica Tais, Stefano Angheben, Andrea Requena Mendez, Ana Muñoz, Jose Nutman, Thomas Bisoffi, Zeno |
author_role |
author |
author2 |
Sequi, Marco Mejia, Rojelio Cimino, Ruben Oscar Krolewiecki, Alejandro Javier Albonico, Marco Degani, Monica Tais, Stefano Angheben, Andrea Requena Mendez, Ana Muñoz, Jose Nutman, Thomas Bisoffi, Zeno |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
STRONGYLOIDES STERCORALIS SEROLOGÍA ELISA GEOHELMINTOS |
topic |
STRONGYLOIDES STERCORALIS SEROLOGÍA ELISA GEOHELMINTOS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cure Fil: Buonfrate, Dora. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia Fil: Sequi, Marco. Mario Negri Institute for Pharmacological Research. Department of Public Health; Italia Fil: Mejia, Rojelio. Public Health Service. National Institute Of Health; Estados Unidos Fil: Cimino, Ruben Oscar. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina Fil: Albonico, Marco. Sacro Cuore Hospital. Center for Tropical Diseases; Italia Fil: Degani, Monica. Sacro Cuore Hospital. Center for Tropical Diseases; Italia Fil: Tais, Stefano. Sacro Cuore Hospital. Center for Tropical Diseases; Italia Fil: Angheben, Andrea. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia Fil: Requena Mendez, Ana. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España Fil: Muñoz, Jose. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España Fil: Nutman, Thomas. Public Health Service. National Institute Of Health; Estados Unidos Fil: Bisoffi, Zeno. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia |
description |
Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cure |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/6988 Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; et al.; Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection; Public Library Of Science; Neglected Tropical Diseases; 9; 2; 10-2-2015; e0003491-e0003491 1935-2735 |
url |
http://hdl.handle.net/11336/6988 |
identifier_str_mv |
Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; et al.; Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection; Public Library Of Science; Neglected Tropical Diseases; 9; 2; 10-2-2015; e0003491-e0003491 1935-2735 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003491 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003491 info:eu-repo/semantics/altIdentifier/pmid/PMC4323101 info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323101/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Of Science |
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Public Library Of Science |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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