Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection

Autores
Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; Albonico, Marco; Degani, Monica; Tais, Stefano; Angheben, Andrea; Requena Mendez, Ana; Muñoz, Jose; Nutman, Thomas; Bisoffi, Zeno
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cure
Fil: Buonfrate, Dora. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
Fil: Sequi, Marco. Mario Negri Institute for Pharmacological Research. Department of Public Health; Italia
Fil: Mejia, Rojelio. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Cimino, Ruben Oscar. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Albonico, Marco. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Degani, Monica. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Tais, Stefano. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Angheben, Andrea. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
Fil: Requena Mendez, Ana. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España
Fil: Muñoz, Jose. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España
Fil: Nutman, Thomas. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Bisoffi, Zeno. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
Materia
STRONGYLOIDES STERCORALIS
SEROLOGÍA
ELISA
GEOHELMINTOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6988
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis InfectionBuonfrate, DoraSequi, MarcoMejia, RojelioCimino, Ruben OscarKrolewiecki, Alejandro JavierAlbonico, MarcoDegani, MonicaTais, StefanoAngheben, AndreaRequena Mendez, AnaMuñoz, JoseNutman, ThomasBisoffi, ZenoSTRONGYLOIDES STERCORALISSEROLOGÍAELISAGEOHELMINTOShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cureFil: Buonfrate, Dora. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; ItaliaFil: Sequi, Marco. Mario Negri Institute for Pharmacological Research. Department of Public Health; ItaliaFil: Mejia, Rojelio. Public Health Service. National Institute Of Health; Estados UnidosFil: Cimino, Ruben Oscar. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Albonico, Marco. Sacro Cuore Hospital. Center for Tropical Diseases; ItaliaFil: Degani, Monica. Sacro Cuore Hospital. Center for Tropical Diseases; ItaliaFil: Tais, Stefano. Sacro Cuore Hospital. Center for Tropical Diseases; ItaliaFil: Angheben, Andrea. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; ItaliaFil: Requena Mendez, Ana. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; EspañaFil: Muñoz, Jose. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; EspañaFil: Nutman, Thomas. Public Health Service. National Institute Of Health; Estados UnidosFil: Bisoffi, Zeno. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; ItaliaPublic Library Of Science2015-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6988Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; et al.; Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection; Public Library Of Science; Neglected Tropical Diseases; 9; 2; 10-2-2015; e0003491-e00034911935-2735enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003491info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003491info:eu-repo/semantics/altIdentifier/pmid/PMC4323101info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323101/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:31Zoai:ri.conicet.gov.ar:11336/6988instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:31.555CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
title Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
spellingShingle Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
Buonfrate, Dora
STRONGYLOIDES STERCORALIS
SEROLOGÍA
ELISA
GEOHELMINTOS
title_short Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
title_full Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
title_fullStr Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
title_full_unstemmed Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
title_sort Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection
dc.creator.none.fl_str_mv Buonfrate, Dora
Sequi, Marco
Mejia, Rojelio
Cimino, Ruben Oscar
Krolewiecki, Alejandro Javier
Albonico, Marco
Degani, Monica
Tais, Stefano
Angheben, Andrea
Requena Mendez, Ana
Muñoz, Jose
Nutman, Thomas
Bisoffi, Zeno
author Buonfrate, Dora
author_facet Buonfrate, Dora
Sequi, Marco
Mejia, Rojelio
Cimino, Ruben Oscar
Krolewiecki, Alejandro Javier
Albonico, Marco
Degani, Monica
Tais, Stefano
Angheben, Andrea
Requena Mendez, Ana
Muñoz, Jose
Nutman, Thomas
Bisoffi, Zeno
author_role author
author2 Sequi, Marco
Mejia, Rojelio
Cimino, Ruben Oscar
Krolewiecki, Alejandro Javier
Albonico, Marco
Degani, Monica
Tais, Stefano
Angheben, Andrea
Requena Mendez, Ana
Muñoz, Jose
Nutman, Thomas
Bisoffi, Zeno
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv STRONGYLOIDES STERCORALIS
SEROLOGÍA
ELISA
GEOHELMINTOS
topic STRONGYLOIDES STERCORALIS
SEROLOGÍA
ELISA
GEOHELMINTOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cure
Fil: Buonfrate, Dora. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
Fil: Sequi, Marco. Mario Negri Institute for Pharmacological Research. Department of Public Health; Italia
Fil: Mejia, Rojelio. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Cimino, Ruben Oscar. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Albonico, Marco. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Degani, Monica. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Tais, Stefano. Sacro Cuore Hospital. Center for Tropical Diseases; Italia
Fil: Angheben, Andrea. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
Fil: Requena Mendez, Ana. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España
Fil: Muñoz, Jose. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia. Centro de Investigación en Salud Internacional de Barcelona; España
Fil: Nutman, Thomas. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Bisoffi, Zeno. Sacro Cuore Hospital. Center for Tropical Diseases; Italia. Coordinating resources to assess and improve Health status of Migrants from Latin America; Italia
description Background Traditional faecal-based methods have poor sensitivity for the detection of S. stercoralis, therefore are inadequate for post-treatment evaluation of infected patients who should be carefully monitored to exclude the persistence of the infection. In a previous study, we demonstrated high accuracy of five serology tests for the screening and diagnosis of strongyloidiasis. Aim of this study is to evaluate the performance of the same five tests for the follow up of patients infected with S. stercoralis. Methods Retrospective study on anonymized, cryo-preserved samples available at the Centre for Tropical Diseases (Negrar, Verona, Italy). Samples were collected before and from 3 to 12 months after treatment. The samples were tested with two commercially-available ELISA tests (IVD, Bordier), two techniques based on a recombinant antigen (NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of each test were evaluated both in relation to the results of fecal examination and to those of a composite reference standard (classifying as positive a sample with positive stools and/or at least three positive serology tests). The associations between the independent variables age and time and the dependent variable value of serological test (for all five tests), were analyzed by linear mixed-effects regression model. Results A high proportion of samples demonstrated for each test a seroreversion or a relevant decline (optical density/relative light units halved or decrease of at least two titers for IFAT) at follow up, results confirmed by the linear mixed effects model that showed a trend to seroreversion over time for all tests. In particular, IVD-ELISA (almost 90% samples demonstrated relevant decline) and IFAT (almost 87%) had the best performance. Considering only samples with a complete negativization, NIE-ELISA showed the best performance (72.5% seroreversion). Conclusions Serology is useful for the follow up of patients infected with S. stercoralis and determining test of cure
publishDate 2015
dc.date.none.fl_str_mv 2015-02-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6988
Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; et al.; Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection; Public Library Of Science; Neglected Tropical Diseases; 9; 2; 10-2-2015; e0003491-e0003491
1935-2735
url http://hdl.handle.net/11336/6988
identifier_str_mv Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Ruben Oscar; Krolewiecki, Alejandro Javier; et al.; Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection; Public Library Of Science; Neglected Tropical Diseases; 9; 2; 10-2-2015; e0003491-e0003491
1935-2735
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003491
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003491
info:eu-repo/semantics/altIdentifier/pmid/PMC4323101
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323101/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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