Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina

Autores
Zabaloy, Maria Celina; Gómez, Marisa Anahi
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our objective was to isolate and characterize indigenous bacteria able to use 2,4-D as a sole carbon (C) and energy source from an agricultural soil in the Sauce Grande River basin (Argentina). Culturable-dependant and molecular methods combined were used to identify and characterize putatively dominant indigenous degrading bacteria. Physiological traits, chloride release and biomass production showed the degradative capacity of the isolates obtained and high-performance liquid chromatography (HPLC) was used to corroborate the evidence. Degrading genes (tfdA and tfdB) were detected in all isolates, and their restriction fragment length polymorphisms (RFLP) were analyzed. Altogether, our results suggest that agricultural use of 2,4-D at recommended level leads to selection for a copiotrophic degrading population. The dominant genus able to metabolize 2,4-D in this soil was identified as Cupriavidus by 16S rRNA gene sequencing and the RFLP profiles of all isolates resembled that of Cupriavidus necator JMP134, the model organism for 2,4-D degradation. The strain EMA-G showed a remarkable performance in herbicide degradation (100 % removal in less than 1 day) in pure culture and is a favorite candidate for future biodegradation experiments.
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Gómez, Marisa Anahi. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Materia
2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Cupriavidus
Tfd Genes
Restriction Fragment Length Polymorphism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11453
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, ArgentinaZabaloy, Maria CelinaGómez, Marisa Anahi2,4-Dichlorophenoxyacetic Acid Degrading BacteriaCupriavidusTfd GenesRestriction Fragment Length Polymorphismhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Our objective was to isolate and characterize indigenous bacteria able to use 2,4-D as a sole carbon (C) and energy source from an agricultural soil in the Sauce Grande River basin (Argentina). Culturable-dependant and molecular methods combined were used to identify and characterize putatively dominant indigenous degrading bacteria. Physiological traits, chloride release and biomass production showed the degradative capacity of the isolates obtained and high-performance liquid chromatography (HPLC) was used to corroborate the evidence. Degrading genes (tfdA and tfdB) were detected in all isolates, and their restriction fragment length polymorphisms (RFLP) were analyzed. Altogether, our results suggest that agricultural use of 2,4-D at recommended level leads to selection for a copiotrophic degrading population. The dominant genus able to metabolize 2,4-D in this soil was identified as Cupriavidus by 16S rRNA gene sequencing and the RFLP profiles of all isolates resembled that of Cupriavidus necator JMP134, the model organism for 2,4-D degradation. The strain EMA-G showed a remarkable performance in herbicide degradation (100 % removal in less than 1 day) in pure culture and is a favorite candidate for future biodegradation experiments.Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Gómez, Marisa Anahi. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaSpringer Verlag Berlín2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11453Zabaloy, Maria Celina; Gómez, Marisa Anahi; Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina; Springer Verlag Berlín; Annals Of Microbiology; 64; 3; 9-2014; 969-9741590-4261enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs13213-013-0731-9info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-013-0731-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:10:36Zoai:ri.conicet.gov.ar:11336/11453instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:10:37.056CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
title Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
spellingShingle Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
Zabaloy, Maria Celina
2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Cupriavidus
Tfd Genes
Restriction Fragment Length Polymorphism
title_short Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
title_full Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
title_fullStr Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
title_full_unstemmed Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
title_sort Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina
dc.creator.none.fl_str_mv Zabaloy, Maria Celina
Gómez, Marisa Anahi
author Zabaloy, Maria Celina
author_facet Zabaloy, Maria Celina
Gómez, Marisa Anahi
author_role author
author2 Gómez, Marisa Anahi
author2_role author
dc.subject.none.fl_str_mv 2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Cupriavidus
Tfd Genes
Restriction Fragment Length Polymorphism
topic 2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Cupriavidus
Tfd Genes
Restriction Fragment Length Polymorphism
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Our objective was to isolate and characterize indigenous bacteria able to use 2,4-D as a sole carbon (C) and energy source from an agricultural soil in the Sauce Grande River basin (Argentina). Culturable-dependant and molecular methods combined were used to identify and characterize putatively dominant indigenous degrading bacteria. Physiological traits, chloride release and biomass production showed the degradative capacity of the isolates obtained and high-performance liquid chromatography (HPLC) was used to corroborate the evidence. Degrading genes (tfdA and tfdB) were detected in all isolates, and their restriction fragment length polymorphisms (RFLP) were analyzed. Altogether, our results suggest that agricultural use of 2,4-D at recommended level leads to selection for a copiotrophic degrading population. The dominant genus able to metabolize 2,4-D in this soil was identified as Cupriavidus by 16S rRNA gene sequencing and the RFLP profiles of all isolates resembled that of Cupriavidus necator JMP134, the model organism for 2,4-D degradation. The strain EMA-G showed a remarkable performance in herbicide degradation (100 % removal in less than 1 day) in pure culture and is a favorite candidate for future biodegradation experiments.
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Gómez, Marisa Anahi. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
description Our objective was to isolate and characterize indigenous bacteria able to use 2,4-D as a sole carbon (C) and energy source from an agricultural soil in the Sauce Grande River basin (Argentina). Culturable-dependant and molecular methods combined were used to identify and characterize putatively dominant indigenous degrading bacteria. Physiological traits, chloride release and biomass production showed the degradative capacity of the isolates obtained and high-performance liquid chromatography (HPLC) was used to corroborate the evidence. Degrading genes (tfdA and tfdB) were detected in all isolates, and their restriction fragment length polymorphisms (RFLP) were analyzed. Altogether, our results suggest that agricultural use of 2,4-D at recommended level leads to selection for a copiotrophic degrading population. The dominant genus able to metabolize 2,4-D in this soil was identified as Cupriavidus by 16S rRNA gene sequencing and the RFLP profiles of all isolates resembled that of Cupriavidus necator JMP134, the model organism for 2,4-D degradation. The strain EMA-G showed a remarkable performance in herbicide degradation (100 % removal in less than 1 day) in pure culture and is a favorite candidate for future biodegradation experiments.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11453
Zabaloy, Maria Celina; Gómez, Marisa Anahi; Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina; Springer Verlag Berlín; Annals Of Microbiology; 64; 3; 9-2014; 969-974
1590-4261
url http://hdl.handle.net/11336/11453
identifier_str_mv Zabaloy, Maria Celina; Gómez, Marisa Anahi; Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina; Springer Verlag Berlín; Annals Of Microbiology; 64; 3; 9-2014; 969-974
1590-4261
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs13213-013-0731-9
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-013-0731-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag Berlín
publisher.none.fl_str_mv Springer Verlag Berlín
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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