Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region

Autores
Cuadrado, Virginia; Gomila, Margarita; Merini, Luciano Jose; Giulietti, Ana Maria; Moore, Edward R. B.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A bacterial consortium able to degrade the herbicide 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB) was obtained from an agricultural soil of the Argentinean Humid Pampa region which has a history of long-term herbicide use. Four bacterial strains were isolated from the consortium and identified as members of the genera Cupriavidus, Labrys and Pseudomonas. A polyphasic systematic analysis was carried out on strain CPDB6T, the member of the 2,4-DB-degrading consortium able to degrade 2,4-DB as a sole carbon and energy source. The Gram-negative, rod-shaped, motile, non-sporulating, non-fermenting bacterium was shown to belong to the genus Cupriavidus on the basis of 16S rRNA gene sequence analyses. Strain CPDB6T did not reduce nitrate, which differentiated it from the type species of the genus, Cupriavidus necator; it did not grow in 0.5–4.5 % NaCl, although most species of Cupriavidus are able to grow at NaCl concentrations as high as 1.5 %; and it was able to deamidate acetamide, which differentiated it from all other species of Cupriavidus. DNA–DNA hybridization data revealed low levels of genomic DNA similarity (less than 30 %) between strain CPDB6T and the type strains of Cupriavidus species with validly published names. The major cellular fatty acids detected were cis-9-hexadecenoic (16 : 1ω7c) and hexadecanoic (16 : 0) acids. On the basis of phenotypic and genotypic characterizations, strain CPDB6T was recognized as a representative of a novel species within the genus Cupriavidus. The name Cupriavidus pampae sp. nov. is proposed, with strain CPDB6T (=CCUG 55948T=CCM-A-29:1289T) as the type strain.
Fil: Cuadrado, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Gomila, Margarita. Universidad de las Islas Baleares; España. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Moore, Edward R. B.. University of Gothenburg; Suecia. Sahlgrenska University Hospital. Department of Clinical Bacteriology; Suecia
Materia
Cupriavidus Pampae
2,4-Db
Argentinean Humid Pampa
Colón Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14400
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14400
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa regionCupriavidus pampae sp. nov., a novel herbicidedegrading bacterium isolated from agricultural soilCuadrado, VirginiaGomila, MargaritaMerini, Luciano JoseGiulietti, Ana MariaMoore, Edward R. B.Cupriavidus Pampae2,4-DbArgentinean Humid PampaColón Controlhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2A bacterial consortium able to degrade the herbicide 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB) was obtained from an agricultural soil of the Argentinean Humid Pampa region which has a history of long-term herbicide use. Four bacterial strains were isolated from the consortium and identified as members of the genera Cupriavidus, Labrys and Pseudomonas. A polyphasic systematic analysis was carried out on strain CPDB6T, the member of the 2,4-DB-degrading consortium able to degrade 2,4-DB as a sole carbon and energy source. The Gram-negative, rod-shaped, motile, non-sporulating, non-fermenting bacterium was shown to belong to the genus Cupriavidus on the basis of 16S rRNA gene sequence analyses. Strain CPDB6T did not reduce nitrate, which differentiated it from the type species of the genus, Cupriavidus necator; it did not grow in 0.5–4.5 % NaCl, although most species of Cupriavidus are able to grow at NaCl concentrations as high as 1.5 %; and it was able to deamidate acetamide, which differentiated it from all other species of Cupriavidus. DNA–DNA hybridization data revealed low levels of genomic DNA similarity (less than 30 %) between strain CPDB6T and the type strains of Cupriavidus species with validly published names. The major cellular fatty acids detected were cis-9-hexadecenoic (16 : 1ω7c) and hexadecanoic (16 : 0) acids. On the basis of phenotypic and genotypic characterizations, strain CPDB6T was recognized as a representative of a novel species within the genus Cupriavidus. The name Cupriavidus pampae sp. nov. is proposed, with strain CPDB6T (=CCUG 55948T=CCM-A-29:1289T) as the type strain.Fil: Cuadrado, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Gomila, Margarita. Universidad de las Islas Baleares; España. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; EspañaFil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Moore, Edward R. B.. University of Gothenburg; Suecia. Sahlgrenska University Hospital. Department of Clinical Bacteriology; SueciaSociety for General Microbiology2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14400Cuadrado, Virginia; Gomila, Margarita; Merini, Luciano Jose; Giulietti, Ana Maria; Moore, Edward R. B.; Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 60; 11; 11-2010; 2606-26121466-50261466-5034enginfo:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.018341-0info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.018341-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:32Zoai:ri.conicet.gov.ar:11336/14400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:33.275CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
Cupriavidus pampae sp. nov., a novel herbicidedegrading bacterium isolated from agricultural soil
title Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
spellingShingle Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
Cuadrado, Virginia
Cupriavidus Pampae
2,4-Db
Argentinean Humid Pampa
Colón Control
title_short Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
title_full Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
title_fullStr Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
title_full_unstemmed Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
title_sort Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region
dc.creator.none.fl_str_mv Cuadrado, Virginia
Gomila, Margarita
Merini, Luciano Jose
Giulietti, Ana Maria
Moore, Edward R. B.
author Cuadrado, Virginia
author_facet Cuadrado, Virginia
Gomila, Margarita
Merini, Luciano Jose
Giulietti, Ana Maria
Moore, Edward R. B.
author_role author
author2 Gomila, Margarita
Merini, Luciano Jose
Giulietti, Ana Maria
Moore, Edward R. B.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cupriavidus Pampae
2,4-Db
Argentinean Humid Pampa
Colón Control
topic Cupriavidus Pampae
2,4-Db
Argentinean Humid Pampa
Colón Control
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A bacterial consortium able to degrade the herbicide 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB) was obtained from an agricultural soil of the Argentinean Humid Pampa region which has a history of long-term herbicide use. Four bacterial strains were isolated from the consortium and identified as members of the genera Cupriavidus, Labrys and Pseudomonas. A polyphasic systematic analysis was carried out on strain CPDB6T, the member of the 2,4-DB-degrading consortium able to degrade 2,4-DB as a sole carbon and energy source. The Gram-negative, rod-shaped, motile, non-sporulating, non-fermenting bacterium was shown to belong to the genus Cupriavidus on the basis of 16S rRNA gene sequence analyses. Strain CPDB6T did not reduce nitrate, which differentiated it from the type species of the genus, Cupriavidus necator; it did not grow in 0.5–4.5 % NaCl, although most species of Cupriavidus are able to grow at NaCl concentrations as high as 1.5 %; and it was able to deamidate acetamide, which differentiated it from all other species of Cupriavidus. DNA–DNA hybridization data revealed low levels of genomic DNA similarity (less than 30 %) between strain CPDB6T and the type strains of Cupriavidus species with validly published names. The major cellular fatty acids detected were cis-9-hexadecenoic (16 : 1ω7c) and hexadecanoic (16 : 0) acids. On the basis of phenotypic and genotypic characterizations, strain CPDB6T was recognized as a representative of a novel species within the genus Cupriavidus. The name Cupriavidus pampae sp. nov. is proposed, with strain CPDB6T (=CCUG 55948T=CCM-A-29:1289T) as the type strain.
Fil: Cuadrado, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Gomila, Margarita. Universidad de las Islas Baleares; España. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Moore, Edward R. B.. University of Gothenburg; Suecia. Sahlgrenska University Hospital. Department of Clinical Bacteriology; Suecia
description A bacterial consortium able to degrade the herbicide 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB) was obtained from an agricultural soil of the Argentinean Humid Pampa region which has a history of long-term herbicide use. Four bacterial strains were isolated from the consortium and identified as members of the genera Cupriavidus, Labrys and Pseudomonas. A polyphasic systematic analysis was carried out on strain CPDB6T, the member of the 2,4-DB-degrading consortium able to degrade 2,4-DB as a sole carbon and energy source. The Gram-negative, rod-shaped, motile, non-sporulating, non-fermenting bacterium was shown to belong to the genus Cupriavidus on the basis of 16S rRNA gene sequence analyses. Strain CPDB6T did not reduce nitrate, which differentiated it from the type species of the genus, Cupriavidus necator; it did not grow in 0.5–4.5 % NaCl, although most species of Cupriavidus are able to grow at NaCl concentrations as high as 1.5 %; and it was able to deamidate acetamide, which differentiated it from all other species of Cupriavidus. DNA–DNA hybridization data revealed low levels of genomic DNA similarity (less than 30 %) between strain CPDB6T and the type strains of Cupriavidus species with validly published names. The major cellular fatty acids detected were cis-9-hexadecenoic (16 : 1ω7c) and hexadecanoic (16 : 0) acids. On the basis of phenotypic and genotypic characterizations, strain CPDB6T was recognized as a representative of a novel species within the genus Cupriavidus. The name Cupriavidus pampae sp. nov. is proposed, with strain CPDB6T (=CCUG 55948T=CCM-A-29:1289T) as the type strain.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14400
Cuadrado, Virginia; Gomila, Margarita; Merini, Luciano Jose; Giulietti, Ana Maria; Moore, Edward R. B.; Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 60; 11; 11-2010; 2606-2612
1466-5026
1466-5034
url http://hdl.handle.net/11336/14400
identifier_str_mv Cuadrado, Virginia; Gomila, Margarita; Merini, Luciano Jose; Giulietti, Ana Maria; Moore, Edward R. B.; Cupriavidus pampae sp. nov.,a novel 4-(2,4-dichlorophenoxy) butyric acid herbicide-degrading bacterium isolated from agricultural soil of the Argentinean Humid Pampa region; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 60; 11; 11-2010; 2606-2612
1466-5026
1466-5034
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.018341-0
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.018341-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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