Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil

Autores
Zabaloy, Maria Celina; Garland, Jay L.; Gómez, Marisa Anahi
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) may influence soil microbial communities by altering the balance between resident populations. Our objective was to assess the effect of environmentally relevant levels (ERLs) of 2,4-D on microbial community function and on the population dynamics of 2,4-D degrading bacteria using a microcosm approach. The most probable number approach was used to enumerate 2,4-D-degrading soil bacteria. Carbon substrates utilization was tested with a microtiter-based oxygen sensor system to evaluate short-term functional shifts caused by herbicide treatment. Shifts in the community in response to potential toxicity of 2,4-D were assessed in the agricultural soil and a reference forest soil using the pollution-induced community-tolerance (PICT) approach. Results indicated that the agricultural soil had a stable 2,4-D degrading population able to use the herbicide as C and energy source, which increases immediately after an ERL dose of 2,4-D and remains high for about 1 month after exposure has ceased. An enhanced, dose-dependent response to 2,4-D as substrate was observed in the microtiter assay, while heterotrophic bacterial activity appeared mostly unchanged. The PICT assay showed higher tolerance to 2,4-D in the agricultural soil than in the unexposed forest soil. Our results suggest that agricultural use of 2,4-D at recommended level leads to selection for (1) a copiotrophic degrader population and (2) a persistently herbicide-tolerant, but functionally similar, microbial community.
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Garland, Jay L.. Kennedy Space Center. Dynamac Corporation; Estados Unidos
Fil: Gómez, Marisa Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Materia
2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Bd Oxygen Biosensor System
Clpp
Pict
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16949
Acceder
id CONICETDig_ac7290a71dd7c2b09cb78886b94f1507
oai_identifier_str oai:ri.conicet.gov.ar:11336/16949
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soilZabaloy, Maria CelinaGarland, Jay L.Gómez, Marisa Anahi2,4-Dichlorophenoxyacetic Acid Degrading BacteriaBd Oxygen Biosensor SystemClppPicthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) may influence soil microbial communities by altering the balance between resident populations. Our objective was to assess the effect of environmentally relevant levels (ERLs) of 2,4-D on microbial community function and on the population dynamics of 2,4-D degrading bacteria using a microcosm approach. The most probable number approach was used to enumerate 2,4-D-degrading soil bacteria. Carbon substrates utilization was tested with a microtiter-based oxygen sensor system to evaluate short-term functional shifts caused by herbicide treatment. Shifts in the community in response to potential toxicity of 2,4-D were assessed in the agricultural soil and a reference forest soil using the pollution-induced community-tolerance (PICT) approach. Results indicated that the agricultural soil had a stable 2,4-D degrading population able to use the herbicide as C and energy source, which increases immediately after an ERL dose of 2,4-D and remains high for about 1 month after exposure has ceased. An enhanced, dose-dependent response to 2,4-D as substrate was observed in the microtiter assay, while heterotrophic bacterial activity appeared mostly unchanged. The PICT assay showed higher tolerance to 2,4-D in the agricultural soil than in the unexposed forest soil. Our results suggest that agricultural use of 2,4-D at recommended level leads to selection for (1) a copiotrophic degrader population and (2) a persistently herbicide-tolerant, but functionally similar, microbial community.Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Garland, Jay L.. Kennedy Space Center. Dynamac Corporation; Estados UnidosFil: Gómez, Marisa Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaElsevier Science2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16949Zabaloy, Maria Celina; Garland, Jay L.; Gómez, Marisa Anahi; Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil; Elsevier Science; Applied Soil Ecology; 46; 2; 10-2010; 240-2460929-1393enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139310001320info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2010.08.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:36Zoai:ri.conicet.gov.ar:11336/16949instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:36.691CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
title Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
spellingShingle Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
Zabaloy, Maria Celina
2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Bd Oxygen Biosensor System
Clpp
Pict
title_short Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
title_full Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
title_fullStr Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
title_full_unstemmed Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
title_sort Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil
dc.creator.none.fl_str_mv Zabaloy, Maria Celina
Garland, Jay L.
Gómez, Marisa Anahi
author Zabaloy, Maria Celina
author_facet Zabaloy, Maria Celina
Garland, Jay L.
Gómez, Marisa Anahi
author_role author
author2 Garland, Jay L.
Gómez, Marisa Anahi
author2_role author
author
dc.subject.none.fl_str_mv 2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Bd Oxygen Biosensor System
Clpp
Pict
topic 2,4-Dichlorophenoxyacetic Acid Degrading Bacteria
Bd Oxygen Biosensor System
Clpp
Pict
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) may influence soil microbial communities by altering the balance between resident populations. Our objective was to assess the effect of environmentally relevant levels (ERLs) of 2,4-D on microbial community function and on the population dynamics of 2,4-D degrading bacteria using a microcosm approach. The most probable number approach was used to enumerate 2,4-D-degrading soil bacteria. Carbon substrates utilization was tested with a microtiter-based oxygen sensor system to evaluate short-term functional shifts caused by herbicide treatment. Shifts in the community in response to potential toxicity of 2,4-D were assessed in the agricultural soil and a reference forest soil using the pollution-induced community-tolerance (PICT) approach. Results indicated that the agricultural soil had a stable 2,4-D degrading population able to use the herbicide as C and energy source, which increases immediately after an ERL dose of 2,4-D and remains high for about 1 month after exposure has ceased. An enhanced, dose-dependent response to 2,4-D as substrate was observed in the microtiter assay, while heterotrophic bacterial activity appeared mostly unchanged. The PICT assay showed higher tolerance to 2,4-D in the agricultural soil than in the unexposed forest soil. Our results suggest that agricultural use of 2,4-D at recommended level leads to selection for (1) a copiotrophic degrader population and (2) a persistently herbicide-tolerant, but functionally similar, microbial community.
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Garland, Jay L.. Kennedy Space Center. Dynamac Corporation; Estados Unidos
Fil: Gómez, Marisa Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
description The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) may influence soil microbial communities by altering the balance between resident populations. Our objective was to assess the effect of environmentally relevant levels (ERLs) of 2,4-D on microbial community function and on the population dynamics of 2,4-D degrading bacteria using a microcosm approach. The most probable number approach was used to enumerate 2,4-D-degrading soil bacteria. Carbon substrates utilization was tested with a microtiter-based oxygen sensor system to evaluate short-term functional shifts caused by herbicide treatment. Shifts in the community in response to potential toxicity of 2,4-D were assessed in the agricultural soil and a reference forest soil using the pollution-induced community-tolerance (PICT) approach. Results indicated that the agricultural soil had a stable 2,4-D degrading population able to use the herbicide as C and energy source, which increases immediately after an ERL dose of 2,4-D and remains high for about 1 month after exposure has ceased. An enhanced, dose-dependent response to 2,4-D as substrate was observed in the microtiter assay, while heterotrophic bacterial activity appeared mostly unchanged. The PICT assay showed higher tolerance to 2,4-D in the agricultural soil than in the unexposed forest soil. Our results suggest that agricultural use of 2,4-D at recommended level leads to selection for (1) a copiotrophic degrader population and (2) a persistently herbicide-tolerant, but functionally similar, microbial community.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16949
Zabaloy, Maria Celina; Garland, Jay L.; Gómez, Marisa Anahi; Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil; Elsevier Science; Applied Soil Ecology; 46; 2; 10-2010; 240-246
0929-1393
url http://hdl.handle.net/11336/16949
identifier_str_mv Zabaloy, Maria Celina; Garland, Jay L.; Gómez, Marisa Anahi; Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil; Elsevier Science; Applied Soil Ecology; 46; 2; 10-2010; 240-246
0929-1393
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139310001320
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2010.08.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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