In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
- Autores
- Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigs
Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Martínez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina - Materia
-
Fosfomycin
In vitro
Penetration, IPEC
Intestinal infections, pigs - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4689
Ver los metadatos del registro completo
id |
CONICETDig_784175240393d8ec6910a5a6fc1033fb |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/4689 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)Pérez, Denisa SoledadMartínez, GuadalupeSoraci, Alejandro LuisTapia, Maria OfeliaFosfomycinIn vitroPenetration, IPECIntestinal infections, pigshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigsFil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Martínez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaRadiance Research Academy2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4689Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2); Radiance Research Academy; International Journal of Medical and Pharmaceutical Sciences; 4; 2; 4-2013; 6-162231- 2188enginfo:eu-repo/semantics/altIdentifier/url/http://www.scopemed.org/?mno=48472info:eu-repo/semantics/altIdentifier/issn/2231- 2188info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:17Zoai:ri.conicet.gov.ar:11336/4689instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:18.003CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) |
title |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) |
spellingShingle |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) Pérez, Denisa Soledad Fosfomycin In vitro Penetration, IPEC Intestinal infections, pigs |
title_short |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) |
title_full |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) |
title_fullStr |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) |
title_full_unstemmed |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) |
title_sort |
In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2) |
dc.creator.none.fl_str_mv |
Pérez, Denisa Soledad Martínez, Guadalupe Soraci, Alejandro Luis Tapia, Maria Ofelia |
author |
Pérez, Denisa Soledad |
author_facet |
Pérez, Denisa Soledad Martínez, Guadalupe Soraci, Alejandro Luis Tapia, Maria Ofelia |
author_role |
author |
author2 |
Martínez, Guadalupe Soraci, Alejandro Luis Tapia, Maria Ofelia |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Fosfomycin In vitro Penetration, IPEC Intestinal infections, pigs |
topic |
Fosfomycin In vitro Penetration, IPEC Intestinal infections, pigs |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigs Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Martínez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina |
description |
Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigs |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4689 Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2); Radiance Research Academy; International Journal of Medical and Pharmaceutical Sciences; 4; 2; 4-2013; 6-16 2231- 2188 |
url |
http://hdl.handle.net/11336/4689 |
identifier_str_mv |
Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2); Radiance Research Academy; International Journal of Medical and Pharmaceutical Sciences; 4; 2; 4-2013; 6-16 2231- 2188 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.scopemed.org/?mno=48472 info:eu-repo/semantics/altIdentifier/issn/2231- 2188 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Radiance Research Academy |
publisher.none.fl_str_mv |
Radiance Research Academy |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842268784907255808 |
score |
13.13397 |