In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)

Autores
Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigs
Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Martínez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Materia
Fosfomycin
In vitro
Penetration, IPEC
Intestinal infections, pigs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4689
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4689
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)Pérez, Denisa SoledadMartínez, GuadalupeSoraci, Alejandro LuisTapia, Maria OfeliaFosfomycinIn vitroPenetration, IPECIntestinal infections, pigshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigsFil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Martínez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaRadiance Research Academy2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4689Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2); Radiance Research Academy; International Journal of Medical and Pharmaceutical Sciences; 4; 2; 4-2013; 6-162231- 2188enginfo:eu-repo/semantics/altIdentifier/url/http://www.scopemed.org/?mno=48472info:eu-repo/semantics/altIdentifier/issn/2231- 2188info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:21Zoai:ri.conicet.gov.ar:11336/4689instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:21.636CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
title In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
spellingShingle In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
Pérez, Denisa Soledad
Fosfomycin
In vitro
Penetration, IPEC
Intestinal infections, pigs
title_short In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
title_full In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
title_fullStr In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
title_full_unstemmed In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
title_sort In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)
dc.creator.none.fl_str_mv Pérez, Denisa Soledad
Martínez, Guadalupe
Soraci, Alejandro Luis
Tapia, Maria Ofelia
author Pérez, Denisa Soledad
author_facet Pérez, Denisa Soledad
Martínez, Guadalupe
Soraci, Alejandro Luis
Tapia, Maria Ofelia
author_role author
author2 Martínez, Guadalupe
Soraci, Alejandro Luis
Tapia, Maria Ofelia
author2_role author
author
author
dc.subject.none.fl_str_mv Fosfomycin
In vitro
Penetration, IPEC
Intestinal infections, pigs
topic Fosfomycin
In vitro
Penetration, IPEC
Intestinal infections, pigs
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigs
Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Martínez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
description Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigs
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4689
Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2); Radiance Research Academy; International Journal of Medical and Pharmaceutical Sciences; 4; 2; 4-2013; 6-16
2231- 2188
url http://hdl.handle.net/11336/4689
identifier_str_mv Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2); Radiance Research Academy; International Journal of Medical and Pharmaceutical Sciences; 4; 2; 4-2013; 6-16
2231- 2188
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scopemed.org/?mno=48472
info:eu-repo/semantics/altIdentifier/issn/2231- 2188
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Radiance Research Academy
publisher.none.fl_str_mv Radiance Research Academy
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613176356765696
score 13.070432

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