Penetration of Fosfomycin into IPEC-J2 Cells in the Presence or Absence of Deoxynivalenol

• Autores: Martínez, Guadalupe; Pérez, Denisa Soledad; Soraci, Alejandro Luis; Tapia, Maria Ofelia
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Fosfomycin; Deoxynivalenol; IPEC-J2 cells; in vitro
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Fosfomycin (FOS) is an antibiotic used in pig farms for treatment and prevention of infections caused by resistant bacteria during the post-weaning period. Antibiotics and non-toxic concentrations of mycotoxins, such as deoxynivalenol (DON) are frequently found in the diet of animals. These compounds can establish interactions in the intestinal tract, which could affect and/or modify the penetration of FOS to enterocytes. The aim of this study was to determine the penetration of FOS into IPECJ-2 cells, a cell line derived from the small intestine of piglets, in the presence and absence of DON. The results from this study showed that there was statistically significant difference in the intracellular concentration of FOS between cells incubated with 580 mg/ml FOS and cells incubated with 580 mg/ml FOS and 1 mg/ml DON. The C max of the intracellular antibiotic in the culture plates incubated with FOS in absence of DON was 45.81 mg/ml with a t max of 4 h. When IPEC-2 cells were incubated with FOS and DON the C max was 20.06 mg/ml and the t max was 30 min. It is concluded that the non-toxic concentration of DON on IPEC-J2 cells after short-term exposure, interferes with the pharmacokinetics of the antibiotic.
Fil: Martínez, Guadalupe. Consejo Nacional de Invest.cientif.y Tecnicas. Ctro Cientifico Tecnologico Conicet - Tandil. Centro de Investigacion Veterinaria de Tandil; Universidad Nacional del Centro de la Provincia de Bs.as.. Facultad de Cs.veterinarias. Departamento de Fisiopatologia. Laboratorio de Toxicologia;
Fil: Pérez, Denisa Soledad. Universidad Nacional del Centro de la Provincia de Bs.as.. Facultad de Cs.veterinarias. Departamento de Fisiopatologia. Laboratorio de Toxicologia; Consejo Nacional de Invest.cientif.y Tecnicas. Ctro Cientifico Tecnologico Conicet - Tandil. Centro de Investigacion Veterinaria de Tandil;
Fil: Soraci, Alejandro Luis. Universidad Nacional del Centro de la Provincia de Bs.as.. Facultad de Cs.veterinarias. Departamento de Fisiopatologia. Laboratorio de Toxicologia; Consejo Nacional de Invest.cientif.y Tecnicas. Ctro Cientifico Tecnologico Conicet - Tandil. Centro de Investigacion Veterinaria de Tandil;
Fil: Tapia, Maria Ofelia. Consejo Nacional de Invest.cientif.y Tecnicas. Ctro Cientifico Tecnologico Conicet - Tandil. Centro de Investigacion Veterinaria de Tandil; Universidad Nacional del Centro de la Provincia de Bs.as.. Facultad de Cs.veterinarias. Departamento de Fisiopatologia. Laboratorio de Toxicologia;