In vitro penetration of Fosfomycin in respiratory cells

Autores
Pérez, Denisa Soledad; Soraci, Alejandro Luis; Tapia, Maria Ofelia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infectious diseases of the respiratory system are important in intensive swine production. Fosfomycin is a widely used antibiotic for the treatment of swine lung infections. The aim of this research was to study the penetration of fosfomycin in an in vitro model of respiratory cells (HEp-2 cells). Cell cultures were subjected to four treatments: a) negative control; b) disodium fosfomycin (280µg/mL); c) disodium fosfomycin (130µg/mL) and d) calcium fosfomycin (130µg/mL). Intracellular concentrations of fosfomycin were analysed by high-performance liquid chromatography tandem mass spectrometry (HPLC MS/MS). Concentrations in HEp-2 cells incubated with 280µg/mL of disodium fosfomycin ranged from 0.74 to 2.79µg/mL (Tmax: 12 hours). When incubated with the same formulation of fosfomycin at a concentration of 130µg/mL, intracellular concentrations ranged between 0.31 and 1.60µg/mL (Tmax: 12 hours). Calcium fosfomycin reached intracellular concentrations that varied between 0.46 and 1.11µg/mL (Tmax: 8 hours). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine respiratory infections (Streptococcus spp.; 0.25µg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intracellular respiratory infections in pigs.
Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia
FOSFOMYCIN
IN VITRO
PIGS
RESPIRATORY CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258619
Acceder
id CONICETDig_89ab99370e7b07312618faa4f4dac8cf
oai_identifier_str oai:ri.conicet.gov.ar:11336/258619
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro penetration of Fosfomycin in respiratory cellsPérez, Denisa SoledadSoraci, Alejandro LuisTapia, Maria OfeliaFOSFOMYCININ VITROPIGSRESPIRATORY CELLShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Infectious diseases of the respiratory system are important in intensive swine production. Fosfomycin is a widely used antibiotic for the treatment of swine lung infections. The aim of this research was to study the penetration of fosfomycin in an in vitro model of respiratory cells (HEp-2 cells). Cell cultures were subjected to four treatments: a) negative control; b) disodium fosfomycin (280µg/mL); c) disodium fosfomycin (130µg/mL) and d) calcium fosfomycin (130µg/mL). Intracellular concentrations of fosfomycin were analysed by high-performance liquid chromatography tandem mass spectrometry (HPLC MS/MS). Concentrations in HEp-2 cells incubated with 280µg/mL of disodium fosfomycin ranged from 0.74 to 2.79µg/mL (Tmax: 12 hours). When incubated with the same formulation of fosfomycin at a concentration of 130µg/mL, intracellular concentrations ranged between 0.31 and 1.60µg/mL (Tmax: 12 hours). Calcium fosfomycin reached intracellular concentrations that varied between 0.46 and 1.11µg/mL (Tmax: 8 hours). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine respiratory infections (Streptococcus spp.; 0.25µg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intracellular respiratory infections in pigs.Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaPig Veterinary Society2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258619Pérez, Denisa Soledad; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of Fosfomycin in respiratory cells; Pig Veterinary Society; The Pig Journal; 67; 6-2012; 43-531352-9749CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.pigvetsoc.org.uk/pig-journalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:26Zoai:ri.conicet.gov.ar:11336/258619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:26.258CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro penetration of Fosfomycin in respiratory cells
title In vitro penetration of Fosfomycin in respiratory cells
spellingShingle In vitro penetration of Fosfomycin in respiratory cells
Pérez, Denisa Soledad
FOSFOMYCIN
IN VITRO
PIGS
RESPIRATORY CELLS
title_short In vitro penetration of Fosfomycin in respiratory cells
title_full In vitro penetration of Fosfomycin in respiratory cells
title_fullStr In vitro penetration of Fosfomycin in respiratory cells
title_full_unstemmed In vitro penetration of Fosfomycin in respiratory cells
title_sort In vitro penetration of Fosfomycin in respiratory cells
dc.creator.none.fl_str_mv Pérez, Denisa Soledad
Soraci, Alejandro Luis
Tapia, Maria Ofelia
author Pérez, Denisa Soledad
author_facet Pérez, Denisa Soledad
Soraci, Alejandro Luis
Tapia, Maria Ofelia
author_role author
author2 Soraci, Alejandro Luis
Tapia, Maria Ofelia
author2_role author
author
dc.subject.none.fl_str_mv FOSFOMYCIN
IN VITRO
PIGS
RESPIRATORY CELLS
topic FOSFOMYCIN
IN VITRO
PIGS
RESPIRATORY CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Infectious diseases of the respiratory system are important in intensive swine production. Fosfomycin is a widely used antibiotic for the treatment of swine lung infections. The aim of this research was to study the penetration of fosfomycin in an in vitro model of respiratory cells (HEp-2 cells). Cell cultures were subjected to four treatments: a) negative control; b) disodium fosfomycin (280µg/mL); c) disodium fosfomycin (130µg/mL) and d) calcium fosfomycin (130µg/mL). Intracellular concentrations of fosfomycin were analysed by high-performance liquid chromatography tandem mass spectrometry (HPLC MS/MS). Concentrations in HEp-2 cells incubated with 280µg/mL of disodium fosfomycin ranged from 0.74 to 2.79µg/mL (Tmax: 12 hours). When incubated with the same formulation of fosfomycin at a concentration of 130µg/mL, intracellular concentrations ranged between 0.31 and 1.60µg/mL (Tmax: 12 hours). Calcium fosfomycin reached intracellular concentrations that varied between 0.46 and 1.11µg/mL (Tmax: 8 hours). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine respiratory infections (Streptococcus spp.; 0.25µg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intracellular respiratory infections in pigs.
Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description Infectious diseases of the respiratory system are important in intensive swine production. Fosfomycin is a widely used antibiotic for the treatment of swine lung infections. The aim of this research was to study the penetration of fosfomycin in an in vitro model of respiratory cells (HEp-2 cells). Cell cultures were subjected to four treatments: a) negative control; b) disodium fosfomycin (280µg/mL); c) disodium fosfomycin (130µg/mL) and d) calcium fosfomycin (130µg/mL). Intracellular concentrations of fosfomycin were analysed by high-performance liquid chromatography tandem mass spectrometry (HPLC MS/MS). Concentrations in HEp-2 cells incubated with 280µg/mL of disodium fosfomycin ranged from 0.74 to 2.79µg/mL (Tmax: 12 hours). When incubated with the same formulation of fosfomycin at a concentration of 130µg/mL, intracellular concentrations ranged between 0.31 and 1.60µg/mL (Tmax: 12 hours). Calcium fosfomycin reached intracellular concentrations that varied between 0.46 and 1.11µg/mL (Tmax: 8 hours). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine respiratory infections (Streptococcus spp.; 0.25µg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intracellular respiratory infections in pigs.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258619
Pérez, Denisa Soledad; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of Fosfomycin in respiratory cells; Pig Veterinary Society; The Pig Journal; 67; 6-2012; 43-53
1352-9749
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258619
identifier_str_mv Pérez, Denisa Soledad; Soraci, Alejandro Luis; Tapia, Maria Ofelia; In vitro penetration of Fosfomycin in respiratory cells; Pig Veterinary Society; The Pig Journal; 67; 6-2012; 43-53
1352-9749
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.pigvetsoc.org.uk/pig-journal
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pig Veterinary Society
publisher.none.fl_str_mv Pig Veterinary Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268665916948480
score 13.13397

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