Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
- Autores
- Barros, S. S.; Gimeno, Eduardo Juan
- Año de publicación
- 2000
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix.
Fil: Barros, S. S.. Universidade Federal de Pelotas; Brasil
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina - Materia
-
Nierembergia veitchii (Nv)
extracellular matrix.
1,25-dihydroxyvitamin D(3)
cellular differentiation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/105132
Ver los metadatos del registro completo
id |
CONICETDig_7812a230db35373b0c0ee8c607699389 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/105132 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous CalcinosisBarros, S. S.Gimeno, Eduardo JuanNierembergia veitchii (Nv)extracellular matrix.1,25-dihydroxyvitamin D(3)cellular differentiationhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix.Fil: Barros, S. S.. Universidade Federal de Pelotas; BrasilFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaElsevier2000-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105132Barros, S. S.; Gimeno, Eduardo Juan; Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis; Elsevier; Journal Of Comparative Pathology; 123; 4; 11-2000; 270-2770021-9975CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021997500904158info:eu-repo/semantics/altIdentifier/doi/10.1053/jcpa.2000.0415info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:33Zoai:ri.conicet.gov.ar:11336/105132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:34.199CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis |
title |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis |
spellingShingle |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis Barros, S. S. Nierembergia veitchii (Nv) extracellular matrix. 1,25-dihydroxyvitamin D(3) cellular differentiation |
title_short |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis |
title_full |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis |
title_fullStr |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis |
title_full_unstemmed |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis |
title_sort |
Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis |
dc.creator.none.fl_str_mv |
Barros, S. S. Gimeno, Eduardo Juan |
author |
Barros, S. S. |
author_facet |
Barros, S. S. Gimeno, Eduardo Juan |
author_role |
author |
author2 |
Gimeno, Eduardo Juan |
author2_role |
author |
dc.subject.none.fl_str_mv |
Nierembergia veitchii (Nv) extracellular matrix. 1,25-dihydroxyvitamin D(3) cellular differentiation |
topic |
Nierembergia veitchii (Nv) extracellular matrix. 1,25-dihydroxyvitamin D(3) cellular differentiation |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix. Fil: Barros, S. S.. Universidade Federal de Pelotas; Brasil Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina |
description |
The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/105132 Barros, S. S.; Gimeno, Eduardo Juan; Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis; Elsevier; Journal Of Comparative Pathology; 123; 4; 11-2000; 270-277 0021-9975 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/105132 |
identifier_str_mv |
Barros, S. S.; Gimeno, Eduardo Juan; Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis; Elsevier; Journal Of Comparative Pathology; 123; 4; 11-2000; 270-277 0021-9975 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021997500904158 info:eu-repo/semantics/altIdentifier/doi/10.1053/jcpa.2000.0415 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613109029797888 |
score |
13.070432 |