Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis

Autores
Barros, S. S.; Gimeno, Eduardo Juan
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix.
Fil: Barros, S. S.. Universidade Federal de Pelotas; Brasil
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
Nierembergia veitchii (Nv)
extracellular matrix.
1,25-dihydroxyvitamin D(3)
cellular differentiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105132
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous CalcinosisBarros, S. S.Gimeno, Eduardo JuanNierembergia veitchii (Nv)extracellular matrix.1,25-dihydroxyvitamin D(3)cellular differentiationhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix.Fil: Barros, S. S.. Universidade Federal de Pelotas; BrasilFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaElsevier2000-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105132Barros, S. S.; Gimeno, Eduardo Juan; Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis; Elsevier; Journal Of Comparative Pathology; 123; 4; 11-2000; 270-2770021-9975CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021997500904158info:eu-repo/semantics/altIdentifier/doi/10.1053/jcpa.2000.0415info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:37Zoai:ri.conicet.gov.ar:11336/105132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:37.992CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
title Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
spellingShingle Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
Barros, S. S.
Nierembergia veitchii (Nv)
extracellular matrix.
1,25-dihydroxyvitamin D(3)
cellular differentiation
title_short Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
title_full Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
title_fullStr Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
title_full_unstemmed Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
title_sort Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis
dc.creator.none.fl_str_mv Barros, S. S.
Gimeno, Eduardo Juan
author Barros, S. S.
author_facet Barros, S. S.
Gimeno, Eduardo Juan
author_role author
author2 Gimeno, Eduardo Juan
author2_role author
dc.subject.none.fl_str_mv Nierembergia veitchii (Nv)
extracellular matrix.
1,25-dihydroxyvitamin D(3)
cellular differentiation
topic Nierembergia veitchii (Nv)
extracellular matrix.
1,25-dihydroxyvitamin D(3)
cellular differentiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix.
Fil: Barros, S. S.. Universidade Federal de Pelotas; Brasil
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description The lungs of three sheep with spontaneous enzootic calcinosis induced by the calcinogenic plantNierembergia veitchii (Nv) were examined electron microscopically and immunohistochemically. The main ultrastructural changes were activation of fibroblasts and modified smooth muscle cells (SMCs) in the pulmonary interstitium, with an increase in extracellular matrix and precipitation of calcium, either in a laminated pattern or as amorphous aggregates. Macrophages and multinucleated giant cells, some with calcium crystals in the cytoplasm, were found in areas of increased extracellular matrix. Thickening and replication of the basal lamina of capillaries were prominent. The bone proteins osteocalcin, osteopontin and osteonectin were detected immunohistochemically in the cytoplasm of activated fibroblasts, in modified SMCs and in the extracellular matrix. It is suggested that 1,25-dihydroxyvitamin D3in Nv induces cellular differentiation and the synthesis of a calcifiable matrix.
publishDate 2000
dc.date.none.fl_str_mv 2000-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105132
Barros, S. S.; Gimeno, Eduardo Juan; Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis; Elsevier; Journal Of Comparative Pathology; 123; 4; 11-2000; 270-277
0021-9975
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105132
identifier_str_mv Barros, S. S.; Gimeno, Eduardo Juan; Cell Differentiation and Bone Protein Synthesis in the Lungs of Sheep with Spontaneous Calcinosis; Elsevier; Journal Of Comparative Pathology; 123; 4; 11-2000; 270-277
0021-9975
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021997500904158
info:eu-repo/semantics/altIdentifier/doi/10.1053/jcpa.2000.0415
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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