1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells

Autores
Irazoqui, Ana Paula; Buitrago, Claudia Graciela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Our research group has recently opened a new investigation line about 1,25(OH)2-vitamin D3 (1,25D) hormone actions in rhabdomyosarcoma cells. Rhabdomyosarcoma is one of soft tissue cancers that affects skeletal muscle and bone, predominantly in kids and older people. Treatments employed to fight this pathology did not advance in the last 30 years. In this work we use the cellular line of human rhabdomyosarcoma, RD, and we evidence that the hormone promotes interesting changes in the cellular cycle. Our data indicate that 1 nM of 1,25D significantly diminishes the amount of live cells at 72 h of treatment (respect its control). When we analyzed the phases of cellular cycle by flow cytometry, we observed that the hormone reduces the number of cells into S phase (at 48 and 72 h of treatment) with a concomitant augment of cells into G0/G1 phase. These results reveal that 1,25D controls rhabdomyosarcoma cells proliferation. To go in deep with these results, we studied the behavior of cellular cycle key proteins by Western blot assays. We determinate that cells treated for 72 h with 1,25D showed an increase in the cyclin D1 and cyclin D3 expression. Of relevance, we evidenced that the hormone augments the protein levels of cyclin dependent kinase inhibitors. Our results indicate that the hormone regulates the cellular cycle of rhabdomyosarcoma cells. Altogether these outcomes open doors to investigate in deep the modulation of different signaling cascades involved in the inhibition of proliferation of these cancer cells exerted by 1,25D.
Fil: Irazoqui, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Buitrago, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
1º Congreso de osteología: XXXIII Reunión anual aaomm: XII Congreso argentino de osteoporosis
Ciudad Autónoma de Buenos Aires
Argentina
Asociación Argentina de Osteología y Metabolismo Mineral
Materia
1,25D
RMS
cell cycle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229464
Acceder
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network_name_str CONICET Digital (CONICET)
spelling 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cellsEfectos del 1a,25(OH)2-vitamina D3 en células de rabdomiosarcoma a nivel del ciclo celularIrazoqui, Ana PaulaBuitrago, Claudia Graciela1,25DRMScell cyclehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Our research group has recently opened a new investigation line about 1,25(OH)2-vitamin D3 (1,25D) hormone actions in rhabdomyosarcoma cells. Rhabdomyosarcoma is one of soft tissue cancers that affects skeletal muscle and bone, predominantly in kids and older people. Treatments employed to fight this pathology did not advance in the last 30 years. In this work we use the cellular line of human rhabdomyosarcoma, RD, and we evidence that the hormone promotes interesting changes in the cellular cycle. Our data indicate that 1 nM of 1,25D significantly diminishes the amount of live cells at 72 h of treatment (respect its control). When we analyzed the phases of cellular cycle by flow cytometry, we observed that the hormone reduces the number of cells into S phase (at 48 and 72 h of treatment) with a concomitant augment of cells into G0/G1 phase. These results reveal that 1,25D controls rhabdomyosarcoma cells proliferation. To go in deep with these results, we studied the behavior of cellular cycle key proteins by Western blot assays. We determinate that cells treated for 72 h with 1,25D showed an increase in the cyclin D1 and cyclin D3 expression. Of relevance, we evidenced that the hormone augments the protein levels of cyclin dependent kinase inhibitors. Our results indicate that the hormone regulates the cellular cycle of rhabdomyosarcoma cells. Altogether these outcomes open doors to investigate in deep the modulation of different signaling cascades involved in the inhibition of proliferation of these cancer cells exerted by 1,25D.Fil: Irazoqui, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Buitrago, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina1º Congreso de osteología: XXXIII Reunión anual aaomm: XII Congreso argentino de osteoporosisCiudad Autónoma de Buenos AiresArgentinaAsociación Argentina de Osteología y Metabolismo MineralSteroidPlantalech, Luisa Carmen2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/2294641alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells; 1º Congreso de osteología: XXXIII Reunión anual aaomm: XII Congreso argentino de osteoporosis; Ciudad Autónoma de Buenos Aires; Argentina; 2016; 300-3001669-8983CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:02Zoai:ri.conicet.gov.ar:11336/229464instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:02.586CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
Efectos del 1a,25(OH)2-vitamina D3 en células de rabdomiosarcoma a nivel del ciclo celular
title 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
spellingShingle 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
Irazoqui, Ana Paula
1,25D
RMS
cell cycle
title_short 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
title_full 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
title_fullStr 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
title_full_unstemmed 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
title_sort 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells
dc.creator.none.fl_str_mv Irazoqui, Ana Paula
Buitrago, Claudia Graciela
author Irazoqui, Ana Paula
author_facet Irazoqui, Ana Paula
Buitrago, Claudia Graciela
author_role author
author2 Buitrago, Claudia Graciela
author2_role author
dc.contributor.none.fl_str_mv Plantalech, Luisa Carmen
dc.subject.none.fl_str_mv 1,25D
RMS
cell cycle
topic 1,25D
RMS
cell cycle
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Our research group has recently opened a new investigation line about 1,25(OH)2-vitamin D3 (1,25D) hormone actions in rhabdomyosarcoma cells. Rhabdomyosarcoma is one of soft tissue cancers that affects skeletal muscle and bone, predominantly in kids and older people. Treatments employed to fight this pathology did not advance in the last 30 years. In this work we use the cellular line of human rhabdomyosarcoma, RD, and we evidence that the hormone promotes interesting changes in the cellular cycle. Our data indicate that 1 nM of 1,25D significantly diminishes the amount of live cells at 72 h of treatment (respect its control). When we analyzed the phases of cellular cycle by flow cytometry, we observed that the hormone reduces the number of cells into S phase (at 48 and 72 h of treatment) with a concomitant augment of cells into G0/G1 phase. These results reveal that 1,25D controls rhabdomyosarcoma cells proliferation. To go in deep with these results, we studied the behavior of cellular cycle key proteins by Western blot assays. We determinate that cells treated for 72 h with 1,25D showed an increase in the cyclin D1 and cyclin D3 expression. Of relevance, we evidenced that the hormone augments the protein levels of cyclin dependent kinase inhibitors. Our results indicate that the hormone regulates the cellular cycle of rhabdomyosarcoma cells. Altogether these outcomes open doors to investigate in deep the modulation of different signaling cascades involved in the inhibition of proliferation of these cancer cells exerted by 1,25D.
Fil: Irazoqui, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Buitrago, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
1º Congreso de osteología: XXXIII Reunión anual aaomm: XII Congreso argentino de osteoporosis
Ciudad Autónoma de Buenos Aires
Argentina
Asociación Argentina de Osteología y Metabolismo Mineral
description Our research group has recently opened a new investigation line about 1,25(OH)2-vitamin D3 (1,25D) hormone actions in rhabdomyosarcoma cells. Rhabdomyosarcoma is one of soft tissue cancers that affects skeletal muscle and bone, predominantly in kids and older people. Treatments employed to fight this pathology did not advance in the last 30 years. In this work we use the cellular line of human rhabdomyosarcoma, RD, and we evidence that the hormone promotes interesting changes in the cellular cycle. Our data indicate that 1 nM of 1,25D significantly diminishes the amount of live cells at 72 h of treatment (respect its control). When we analyzed the phases of cellular cycle by flow cytometry, we observed that the hormone reduces the number of cells into S phase (at 48 and 72 h of treatment) with a concomitant augment of cells into G0/G1 phase. These results reveal that 1,25D controls rhabdomyosarcoma cells proliferation. To go in deep with these results, we studied the behavior of cellular cycle key proteins by Western blot assays. We determinate that cells treated for 72 h with 1,25D showed an increase in the cyclin D1 and cyclin D3 expression. Of relevance, we evidenced that the hormone augments the protein levels of cyclin dependent kinase inhibitors. Our results indicate that the hormone regulates the cellular cycle of rhabdomyosarcoma cells. Altogether these outcomes open doors to investigate in deep the modulation of different signaling cascades involved in the inhibition of proliferation of these cancer cells exerted by 1,25D.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229464
1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells; 1º Congreso de osteología: XXXIII Reunión anual aaomm: XII Congreso argentino de osteoporosis; Ciudad Autónoma de Buenos Aires; Argentina; 2016; 300-300
1669-8983
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229464
identifier_str_mv 1alpha, 25(OH)2-vitamin D3 effects in cellular cycle of rhabdomyosarcoma cells; 1º Congreso de osteología: XXXIII Reunión anual aaomm: XII Congreso argentino de osteoporosis; Ciudad Autónoma de Buenos Aires; Argentina; 2016; 300-300
1669-8983
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Steroid
publisher.none.fl_str_mv Steroid
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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