Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator

Autores
García, I. L.; Lopez Jimenez, Jimmy Andres; Dorado, M. P.; Kopsahelis, N.; Alexandri, M.; Papanikolaou, S.; Villar, Marcelo Armando; Koutinas, A. A.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (PHA) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. Batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) with a 2.8–8% 3HV content. Fed-batch fermentations in shake flasks led to the production of 10.9 g/L P(3HB-co-3HV) and a 55.6% P(3HB-co-3HV) content. NaCl concentrations between 2 and 6 g/L gradually became inhibitory to bacterial growth and PHA formation, whereas in the case of K2SO4, the inhibitory effect was observed only at concentrations higher than 20 g/L. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and nuclear magnetic resonance (13C NMR) demonstrated that the incorporation of 3HV into the obtained P(3HB-co-3HV) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. Integrating PHA production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery.
Fil: García, I. L.. Universidad de Córdoba; España
Fil: Lopez Jimenez, Jimmy Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Dorado, M. P.. Universidad de Córdoba; España
Fil: Kopsahelis, N.. Agricultural University of Athens; Grecia
Fil: Alexandri, M.. Agricultural University of Athens; Grecia
Fil: Papanikolaou, S.. Agricultural University of Athens; Grecia
Fil: Villar, Marcelo Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Koutinas, A. A.. Agricultural University of Athens; Grecia
Materia
Crude Glycerol
Rapeseed Meal Hydrolysate
Microbial Bioconversion
Cupriavidus Necator
Polyhydroxyvalkanoates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11963
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11963
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necatorGarcía, I. L.Lopez Jimenez, Jimmy AndresDorado, M. P.Kopsahelis, N.Alexandri, M.Papanikolaou, S.Villar, Marcelo ArmandoKoutinas, A. A.Crude GlycerolRapeseed Meal HydrolysateMicrobial BioconversionCupriavidus NecatorPolyhydroxyvalkanoateshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (PHA) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. Batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) with a 2.8–8% 3HV content. Fed-batch fermentations in shake flasks led to the production of 10.9 g/L P(3HB-co-3HV) and a 55.6% P(3HB-co-3HV) content. NaCl concentrations between 2 and 6 g/L gradually became inhibitory to bacterial growth and PHA formation, whereas in the case of K2SO4, the inhibitory effect was observed only at concentrations higher than 20 g/L. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and nuclear magnetic resonance (13C NMR) demonstrated that the incorporation of 3HV into the obtained P(3HB-co-3HV) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. Integrating PHA production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery.Fil: García, I. L.. Universidad de Córdoba; EspañaFil: Lopez Jimenez, Jimmy Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Dorado, M. P.. Universidad de Córdoba; EspañaFil: Kopsahelis, N.. Agricultural University of Athens; GreciaFil: Alexandri, M.. Agricultural University of Athens; GreciaFil: Papanikolaou, S.. Agricultural University of Athens; GreciaFil: Villar, Marcelo Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Koutinas, A. A.. Agricultural University of Athens; GreciaElsevier2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11963García, I. L.; Lopez Jimenez, Jimmy Andres; Dorado, M. P.; Kopsahelis, N.; Alexandri, M.; et al.; Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator; Elsevier; Bioresource Technology; 130; 2-2013; 16-220960-8524enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S096085241201783Xinfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.biortech.2012.11.088info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:54Zoai:ri.conicet.gov.ar:11336/11963instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:55.075CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
title Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
spellingShingle Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
García, I. L.
Crude Glycerol
Rapeseed Meal Hydrolysate
Microbial Bioconversion
Cupriavidus Necator
Polyhydroxyvalkanoates
title_short Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
title_full Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
title_fullStr Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
title_full_unstemmed Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
title_sort Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
dc.creator.none.fl_str_mv García, I. L.
Lopez Jimenez, Jimmy Andres
Dorado, M. P.
Kopsahelis, N.
Alexandri, M.
Papanikolaou, S.
Villar, Marcelo Armando
Koutinas, A. A.
author García, I. L.
author_facet García, I. L.
Lopez Jimenez, Jimmy Andres
Dorado, M. P.
Kopsahelis, N.
Alexandri, M.
Papanikolaou, S.
Villar, Marcelo Armando
Koutinas, A. A.
author_role author
author2 Lopez Jimenez, Jimmy Andres
Dorado, M. P.
Kopsahelis, N.
Alexandri, M.
Papanikolaou, S.
Villar, Marcelo Armando
Koutinas, A. A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Crude Glycerol
Rapeseed Meal Hydrolysate
Microbial Bioconversion
Cupriavidus Necator
Polyhydroxyvalkanoates
topic Crude Glycerol
Rapeseed Meal Hydrolysate
Microbial Bioconversion
Cupriavidus Necator
Polyhydroxyvalkanoates
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (PHA) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. Batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) with a 2.8–8% 3HV content. Fed-batch fermentations in shake flasks led to the production of 10.9 g/L P(3HB-co-3HV) and a 55.6% P(3HB-co-3HV) content. NaCl concentrations between 2 and 6 g/L gradually became inhibitory to bacterial growth and PHA formation, whereas in the case of K2SO4, the inhibitory effect was observed only at concentrations higher than 20 g/L. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and nuclear magnetic resonance (13C NMR) demonstrated that the incorporation of 3HV into the obtained P(3HB-co-3HV) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. Integrating PHA production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery.
Fil: García, I. L.. Universidad de Córdoba; España
Fil: Lopez Jimenez, Jimmy Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Dorado, M. P.. Universidad de Córdoba; España
Fil: Kopsahelis, N.. Agricultural University of Athens; Grecia
Fil: Alexandri, M.. Agricultural University of Athens; Grecia
Fil: Papanikolaou, S.. Agricultural University of Athens; Grecia
Fil: Villar, Marcelo Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Koutinas, A. A.. Agricultural University of Athens; Grecia
description Utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (PHA) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. Batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) with a 2.8–8% 3HV content. Fed-batch fermentations in shake flasks led to the production of 10.9 g/L P(3HB-co-3HV) and a 55.6% P(3HB-co-3HV) content. NaCl concentrations between 2 and 6 g/L gradually became inhibitory to bacterial growth and PHA formation, whereas in the case of K2SO4, the inhibitory effect was observed only at concentrations higher than 20 g/L. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and nuclear magnetic resonance (13C NMR) demonstrated that the incorporation of 3HV into the obtained P(3HB-co-3HV) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. Integrating PHA production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11963
García, I. L.; Lopez Jimenez, Jimmy Andres; Dorado, M. P.; Kopsahelis, N.; Alexandri, M.; et al.; Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator; Elsevier; Bioresource Technology; 130; 2-2013; 16-22
0960-8524
url http://hdl.handle.net/11336/11963
identifier_str_mv García, I. L.; Lopez Jimenez, Jimmy Andres; Dorado, M. P.; Kopsahelis, N.; Alexandri, M.; et al.; Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator; Elsevier; Bioresource Technology; 130; 2-2013; 16-22
0960-8524
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S096085241201783X
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.biortech.2012.11.088
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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