Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects

Autores
Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Merini, Luciano Jose; Martinez Pabón, María Cecilia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effectsDental caries is a pathology of multifactorial origin and currently natural products are an efficientalternative treatment. The work sought to evaluate the antimicrobial activity of the three substances(Cymbopogon citratus essential oil and the citral and myrcene components) against Streptococcus mutans ATCCUA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. Through Scanning electron microscopy,the typical morphology of S. mutans was observed and, a characteristic formation of polysaccharides, withmore structured arrangement at 48 h. The bacterial viability effect against S. mutans on biofilms was evaluatedthrough exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1,0.1, and 0.01 μg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated onkeratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control(negative control) and ethanol 1 % as vehicle control (positive control). The three substances evaluated hadeffects on the bacterial viability of S. mutans with mortality between 74 and 96 %, without significant differenceamong them (P > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studiedfurther as potential co-adjuvants to prevent dental caries that cause minor adverse effects.
Fil: Ortega Cuadros, Mailen. Universidad de Antioquia; Colombia
Fil: Tofiño Rivera, Adriana Patricia. Universidad de Santander; Colombia. Centro de Investigación Motilonia de AGROSAVIA; Colombia
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Martinez Pabón, María Cecilia. Universidad de Antioquia; Colombia
Materia
ACEITES ESENCIALES
CARIES
CYMBOPOGON CITRATUS
STREPTOCOCUS MUTANS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104150
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effectsOrtega Cuadros, MailenTofiño Rivera, Adriana PatriciaMerini, Luciano JoseMartinez Pabón, María CeciliaACEITES ESENCIALESCARIESCYMBOPOGON CITRATUSSTREPTOCOCUS MUTANShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effectsDental caries is a pathology of multifactorial origin and currently natural products are an efficientalternative treatment. The work sought to evaluate the antimicrobial activity of the three substances(Cymbopogon citratus essential oil and the citral and myrcene components) against Streptococcus mutans ATCCUA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. Through Scanning electron microscopy,the typical morphology of S. mutans was observed and, a characteristic formation of polysaccharides, withmore structured arrangement at 48 h. The bacterial viability effect against S. mutans on biofilms was evaluatedthrough exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1,0.1, and 0.01 μg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated onkeratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control(negative control) and ethanol 1 % as vehicle control (positive control). The three substances evaluated hadeffects on the bacterial viability of S. mutans with mortality between 74 and 96 %, without significant differenceamong them (P > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studiedfurther as potential co-adjuvants to prevent dental caries that cause minor adverse effects.Fil: Ortega Cuadros, Mailen. Universidad de Antioquia; ColombiaFil: Tofiño Rivera, Adriana Patricia. Universidad de Santander; Colombia. Centro de Investigación Motilonia de AGROSAVIA; ColombiaFil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; ArgentinaFil: Martinez Pabón, María Cecilia. Universidad de Antioquia; ColombiaRevista de Biología Tropical2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104150Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Merini, Luciano Jose; Martinez Pabón, María Cecilia; Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects; Revista de Biología Tropical; Revista de Biología Tropical; 66; 4; 12-2018; 1519-15290034-7744CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:06Zoai:ri.conicet.gov.ar:11336/104150instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:07.202CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
title Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
spellingShingle Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
Ortega Cuadros, Mailen
ACEITES ESENCIALES
CARIES
CYMBOPOGON CITRATUS
STREPTOCOCUS MUTANS
title_short Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
title_full Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
title_fullStr Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
title_full_unstemmed Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
title_sort Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
dc.creator.none.fl_str_mv Ortega Cuadros, Mailen
Tofiño Rivera, Adriana Patricia
Merini, Luciano Jose
Martinez Pabón, María Cecilia
author Ortega Cuadros, Mailen
author_facet Ortega Cuadros, Mailen
Tofiño Rivera, Adriana Patricia
Merini, Luciano Jose
Martinez Pabón, María Cecilia
author_role author
author2 Tofiño Rivera, Adriana Patricia
Merini, Luciano Jose
Martinez Pabón, María Cecilia
author2_role author
author
author
dc.subject.none.fl_str_mv ACEITES ESENCIALES
CARIES
CYMBOPOGON CITRATUS
STREPTOCOCUS MUTANS
topic ACEITES ESENCIALES
CARIES
CYMBOPOGON CITRATUS
STREPTOCOCUS MUTANS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effectsDental caries is a pathology of multifactorial origin and currently natural products are an efficientalternative treatment. The work sought to evaluate the antimicrobial activity of the three substances(Cymbopogon citratus essential oil and the citral and myrcene components) against Streptococcus mutans ATCCUA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. Through Scanning electron microscopy,the typical morphology of S. mutans was observed and, a characteristic formation of polysaccharides, withmore structured arrangement at 48 h. The bacterial viability effect against S. mutans on biofilms was evaluatedthrough exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1,0.1, and 0.01 μg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated onkeratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control(negative control) and ethanol 1 % as vehicle control (positive control). The three substances evaluated hadeffects on the bacterial viability of S. mutans with mortality between 74 and 96 %, without significant differenceamong them (P > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studiedfurther as potential co-adjuvants to prevent dental caries that cause minor adverse effects.
Fil: Ortega Cuadros, Mailen. Universidad de Antioquia; Colombia
Fil: Tofiño Rivera, Adriana Patricia. Universidad de Santander; Colombia. Centro de Investigación Motilonia de AGROSAVIA; Colombia
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Martinez Pabón, María Cecilia. Universidad de Antioquia; Colombia
description Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effectsDental caries is a pathology of multifactorial origin and currently natural products are an efficientalternative treatment. The work sought to evaluate the antimicrobial activity of the three substances(Cymbopogon citratus essential oil and the citral and myrcene components) against Streptococcus mutans ATCCUA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. Through Scanning electron microscopy,the typical morphology of S. mutans was observed and, a characteristic formation of polysaccharides, withmore structured arrangement at 48 h. The bacterial viability effect against S. mutans on biofilms was evaluatedthrough exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1,0.1, and 0.01 μg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated onkeratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control(negative control) and ethanol 1 % as vehicle control (positive control). The three substances evaluated hadeffects on the bacterial viability of S. mutans with mortality between 74 and 96 %, without significant differenceamong them (P > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studiedfurther as potential co-adjuvants to prevent dental caries that cause minor adverse effects.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104150
Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Merini, Luciano Jose; Martinez Pabón, María Cecilia; Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects; Revista de Biología Tropical; Revista de Biología Tropical; 66; 4; 12-2018; 1519-1529
0034-7744
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104150
identifier_str_mv Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Merini, Luciano Jose; Martinez Pabón, María Cecilia; Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects; Revista de Biología Tropical; Revista de Biología Tropical; 66; 4; 12-2018; 1519-1529
0034-7744
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Revista de Biología Tropical
publisher.none.fl_str_mv Revista de Biología Tropical
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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