Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells

Autores
Tofiño Rivera, Adriana Patricia; Ortega Cuadros, Mailen; Galvis Pareja, David Andrés; Jimenez Rios, Hedilka; Merini, Luciano J.; Martínez Pabón, María Cecilia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology: Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results: The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries.
EEA Anguil
Fil: Tofiño Rivera, Adriana Patricia. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; Colombia
Fil: Ortega Cuadros, Mailen. Universidad Popular del Cesar; Colombia
Fil: Galvis Pareja, David Andrés. Universidad CES; Colombia
Fil: Jimenez Rios, Hedilka. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; Colombia
Fil: Merini, Luciano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Martínez Pabón, María Cecilia. Universidad de Antioquía. Facultad de Odontología. Laboratorio de Microbiología Bucal. Grupo de Investigación en Patología Oral, Periodoncia y Cirugía Alvéolo-Dentaria; Colombia
Fuente
Journal of ethnopharmacology 194 : 749-754. (24 December 2016)
Materia
Cymbopogon Citratus
Streptococcus Mutans
Citotoxicidad
Caries Dental
Contaminación Biológica
Aceites Esenciales
Cytotoxicity
Dental Caries
Biological Contamination
Essential Oils
Lippia Alba
Biofilms
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3908
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3908
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network_name_str INTA Digital (INTA)
spelling Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cellsTofiño Rivera, Adriana PatriciaOrtega Cuadros, MailenGalvis Pareja, David AndrésJimenez Rios, HedilkaMerini, Luciano J.Martínez Pabón, María CeciliaCymbopogon CitratusStreptococcus MutansCitotoxicidadCaries DentalContaminación BiológicaAceites EsencialesCytotoxicityDental CariesBiological ContaminationEssential OilsLippia AlbaBiofilmsBackground: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology: Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results: The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries.EEA AnguilFil: Tofiño Rivera, Adriana Patricia. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; ColombiaFil: Ortega Cuadros, Mailen. Universidad Popular del Cesar; ColombiaFil: Galvis Pareja, David Andrés. Universidad CES; ColombiaFil: Jimenez Rios, Hedilka. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; ColombiaFil: Merini, Luciano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Martínez Pabón, María Cecilia. Universidad de Antioquía. Facultad de Odontología. Laboratorio de Microbiología Bucal. Grupo de Investigación en Patología Oral, Periodoncia y Cirugía Alvéolo-Dentaria; ColombiaElsevier2018-11-15T17:00:50Z2018-11-15T17:00:50Z2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3908https://www.sciencedirect.com/science/article/pii/S0378874116313289?via%3Dihub0378-8741https://doi.org/10.1016/j.jep.2016.10.044Journal of ethnopharmacology 194 : 749-754. (24 December 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:45Zoai:localhost:20.500.12123/3908instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:45.303INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
title Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
spellingShingle Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
Tofiño Rivera, Adriana Patricia
Cymbopogon Citratus
Streptococcus Mutans
Citotoxicidad
Caries Dental
Contaminación Biológica
Aceites Esenciales
Cytotoxicity
Dental Caries
Biological Contamination
Essential Oils
Lippia Alba
Biofilms
title_short Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
title_full Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
title_fullStr Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
title_full_unstemmed Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
title_sort Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
dc.creator.none.fl_str_mv Tofiño Rivera, Adriana Patricia
Ortega Cuadros, Mailen
Galvis Pareja, David Andrés
Jimenez Rios, Hedilka
Merini, Luciano J.
Martínez Pabón, María Cecilia
author Tofiño Rivera, Adriana Patricia
author_facet Tofiño Rivera, Adriana Patricia
Ortega Cuadros, Mailen
Galvis Pareja, David Andrés
Jimenez Rios, Hedilka
Merini, Luciano J.
Martínez Pabón, María Cecilia
author_role author
author2 Ortega Cuadros, Mailen
Galvis Pareja, David Andrés
Jimenez Rios, Hedilka
Merini, Luciano J.
Martínez Pabón, María Cecilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cymbopogon Citratus
Streptococcus Mutans
Citotoxicidad
Caries Dental
Contaminación Biológica
Aceites Esenciales
Cytotoxicity
Dental Caries
Biological Contamination
Essential Oils
Lippia Alba
Biofilms
topic Cymbopogon Citratus
Streptococcus Mutans
Citotoxicidad
Caries Dental
Contaminación Biológica
Aceites Esenciales
Cytotoxicity
Dental Caries
Biological Contamination
Essential Oils
Lippia Alba
Biofilms
dc.description.none.fl_txt_mv Background: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology: Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results: The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries.
EEA Anguil
Fil: Tofiño Rivera, Adriana Patricia. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; Colombia
Fil: Ortega Cuadros, Mailen. Universidad Popular del Cesar; Colombia
Fil: Galvis Pareja, David Andrés. Universidad CES; Colombia
Fil: Jimenez Rios, Hedilka. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; Colombia
Fil: Merini, Luciano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Martínez Pabón, María Cecilia. Universidad de Antioquía. Facultad de Odontología. Laboratorio de Microbiología Bucal. Grupo de Investigación en Patología Oral, Periodoncia y Cirugía Alvéolo-Dentaria; Colombia
description Background: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology: Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results: The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
2018-11-15T17:00:50Z
2018-11-15T17:00:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3908
https://www.sciencedirect.com/science/article/pii/S0378874116313289?via%3Dihub
0378-8741
https://doi.org/10.1016/j.jep.2016.10.044
url http://hdl.handle.net/20.500.12123/3908
https://www.sciencedirect.com/science/article/pii/S0378874116313289?via%3Dihub
https://doi.org/10.1016/j.jep.2016.10.044
identifier_str_mv 0378-8741
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of ethnopharmacology 194 : 749-754. (24 December 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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