Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)

Autores
Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism.
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: del Valle, Juana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Michiels, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
Intertidal Euryhaline Crabs
Neohelice Granulata
Dopamine
Glucagon
Camp
Hepatopancreas
Enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39856
Acceder
id CONICETDig_76ac1b076d1611cb197d63c8a4916f64
oai_identifier_str oai:ri.conicet.gov.ar:11336/39856
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)Lopez Mañanes, Alejandra Antoniadel Valle, Juana CristinaMichiels, Maria SoledadIntertidal Euryhaline CrabsNeohelice GranulataDopamineGlucagonCampHepatopancreasEnzymeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism.Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: del Valle, Juana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Michiels, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaBrill Academic Publishers2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39856Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad; Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae); Brill Academic Publishers; Crustaceana; 88; 1; 1-2015; 51-650011-216XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1163/15685403-00003399info:eu-repo/semantics/altIdentifier/url/http://booksandjournals.brillonline.com/content/journals/10.1163/15685403-00003399info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:39Zoai:ri.conicet.gov.ar:11336/39856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:40.18CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
spellingShingle Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
Lopez Mañanes, Alejandra Antonia
Intertidal Euryhaline Crabs
Neohelice Granulata
Dopamine
Glucagon
Camp
Hepatopancreas
Enzymes
title_short Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_full Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_fullStr Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_full_unstemmed Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_sort Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
dc.creator.none.fl_str_mv Lopez Mañanes, Alejandra Antonia
del Valle, Juana Cristina
Michiels, Maria Soledad
author Lopez Mañanes, Alejandra Antonia
author_facet Lopez Mañanes, Alejandra Antonia
del Valle, Juana Cristina
Michiels, Maria Soledad
author_role author
author2 del Valle, Juana Cristina
Michiels, Maria Soledad
author2_role author
author
dc.subject.none.fl_str_mv Intertidal Euryhaline Crabs
Neohelice Granulata
Dopamine
Glucagon
Camp
Hepatopancreas
Enzymes
topic Intertidal Euryhaline Crabs
Neohelice Granulata
Dopamine
Glucagon
Camp
Hepatopancreas
Enzymes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism.
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: del Valle, Juana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Michiels, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39856
Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad; Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae); Brill Academic Publishers; Crustaceana; 88; 1; 1-2015; 51-65
0011-216X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39856
identifier_str_mv Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad; Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae); Brill Academic Publishers; Crustaceana; 88; 1; 1-2015; 51-65
0011-216X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1163/15685403-00003399
info:eu-repo/semantics/altIdentifier/url/http://booksandjournals.brillonline.com/content/journals/10.1163/15685403-00003399
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Brill Academic Publishers
publisher.none.fl_str_mv Brill Academic Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269045673426944
score 13.13397

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