Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding

Autores
Méndez, Eugenia; Caruso Neves, C.; Lopez Mañanes, Alejandra Antonia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
No study has been done on the existence, biochemical characteristics, and modulation of K+-independent ouabain-insensitive Na+ ATPase activity (the second sodium pump) in the digestive tract of intertidal euryhaline crabs and moreover on the coexistence and modulation under distinct physiological and (or) environmental conditions of different sodium pumps. We determined the occurrence, characteristics, and responses at different times (0, 1, 24, 48, and 120 h) after feeding upon distinct salinities of Na+ ATPase activity and Na+/K+ ATPase in the hepatopancreas of Neohelice granulata (Dana, 1851), which is a model species. The stimulation by Na+ under total inhibition of Na+/K+ ATPase activity revealed the occurrence of Na+ ATPase activity that was totally inhibited by 2 mmol·L–1 furosemide, exhibits Michaelis–Menten kinetics for ATP (apparent Km = 0.52 ± 0.16 mmol·L–1), and highest activity at around pH 7.4. In crabs acclimated to 35 psu (osmoconforming conditions), Na+ ATPase activity was highly increased (about 15-fold) (532 ± 58 nmol Pi·mg protein−1·min−1) in the hepatopancreas 48 h after feeding. In 10 psu (hyper-regulating conditions), Na+ ATPase activity decreased in the hepatopancreas 24 h after feeding (7 ± 9 nmol Pi·mg protein−1·min−1) and recovered initial values after 48 h (24 ± 35 nmol Pi·mg protein−1·min−1). Unlike Na+ ATPase, Na+/K+ ATPase activity did not change after feeding at any salinity, suggesting the specific modulation of the second sodium pump and its role in postprandial adjustments in the hepatopancreas.
Fil: Méndez, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Caruso Neves, C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
CRAB
FEEDING
HEPATOPANCREAS
K+-INDEPENDENT OUABAIN-INSENSITIVE NA+ ATPASE ACTIVITY
NEOHELICE GRANULATA
SODIUM PUMPS
SOUTHWESTERN ATLANTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92754
Acceder
id CONICETDig_b418acc55f49c22a5df8634555a723e7
oai_identifier_str oai:ri.conicet.gov.ar:11336/92754
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feedingMéndez, EugeniaCaruso Neves, C.Lopez Mañanes, Alejandra AntoniaCRABFEEDINGHEPATOPANCREASK+-INDEPENDENT OUABAIN-INSENSITIVE NA+ ATPASE ACTIVITYNEOHELICE GRANULATASODIUM PUMPSSOUTHWESTERN ATLANTIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1No study has been done on the existence, biochemical characteristics, and modulation of K+-independent ouabain-insensitive Na+ ATPase activity (the second sodium pump) in the digestive tract of intertidal euryhaline crabs and moreover on the coexistence and modulation under distinct physiological and (or) environmental conditions of different sodium pumps. We determined the occurrence, characteristics, and responses at different times (0, 1, 24, 48, and 120 h) after feeding upon distinct salinities of Na+ ATPase activity and Na+/K+ ATPase in the hepatopancreas of Neohelice granulata (Dana, 1851), which is a model species. The stimulation by Na+ under total inhibition of Na+/K+ ATPase activity revealed the occurrence of Na+ ATPase activity that was totally inhibited by 2 mmol·L–1 furosemide, exhibits Michaelis–Menten kinetics for ATP (apparent Km = 0.52 ± 0.16 mmol·L–1), and highest activity at around pH 7.4. In crabs acclimated to 35 psu (osmoconforming conditions), Na+ ATPase activity was highly increased (about 15-fold) (532 ± 58 nmol Pi·mg protein−1·min−1) in the hepatopancreas 48 h after feeding. In 10 psu (hyper-regulating conditions), Na+ ATPase activity decreased in the hepatopancreas 24 h after feeding (7 ± 9 nmol Pi·mg protein−1·min−1) and recovered initial values after 48 h (24 ± 35 nmol Pi·mg protein−1·min−1). Unlike Na+ ATPase, Na+/K+ ATPase activity did not change after feeding at any salinity, suggesting the specific modulation of the second sodium pump and its role in postprandial adjustments in the hepatopancreas.Fil: Méndez, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Caruso Neves, C.. Universidade Federal do Rio de Janeiro; BrasilFil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaNational Research Council Canada-NRC Research Press2018-01-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92754Méndez, Eugenia; Caruso Neves, C.; Lopez Mañanes, Alejandra Antonia; Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 96; 6; 5-1-2018; 576-5840008-4301CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/cjz-2017-0128info:eu-repo/semantics/altIdentifier/doi/10.1139/cjz-2017-0128info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:01Zoai:ri.conicet.gov.ar:11336/92754instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:01.647CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
title Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
spellingShingle Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
Méndez, Eugenia
CRAB
FEEDING
HEPATOPANCREAS
K+-INDEPENDENT OUABAIN-INSENSITIVE NA+ ATPASE ACTIVITY
NEOHELICE GRANULATA
SODIUM PUMPS
SOUTHWESTERN ATLANTIC
title_short Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
title_full Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
title_fullStr Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
title_full_unstemmed Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
title_sort Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding
dc.creator.none.fl_str_mv Méndez, Eugenia
Caruso Neves, C.
Lopez Mañanes, Alejandra Antonia
author Méndez, Eugenia
author_facet Méndez, Eugenia
Caruso Neves, C.
Lopez Mañanes, Alejandra Antonia
author_role author
author2 Caruso Neves, C.
Lopez Mañanes, Alejandra Antonia
author2_role author
author
dc.subject.none.fl_str_mv CRAB
FEEDING
HEPATOPANCREAS
K+-INDEPENDENT OUABAIN-INSENSITIVE NA+ ATPASE ACTIVITY
NEOHELICE GRANULATA
SODIUM PUMPS
SOUTHWESTERN ATLANTIC
topic CRAB
FEEDING
HEPATOPANCREAS
K+-INDEPENDENT OUABAIN-INSENSITIVE NA+ ATPASE ACTIVITY
NEOHELICE GRANULATA
SODIUM PUMPS
SOUTHWESTERN ATLANTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv No study has been done on the existence, biochemical characteristics, and modulation of K+-independent ouabain-insensitive Na+ ATPase activity (the second sodium pump) in the digestive tract of intertidal euryhaline crabs and moreover on the coexistence and modulation under distinct physiological and (or) environmental conditions of different sodium pumps. We determined the occurrence, characteristics, and responses at different times (0, 1, 24, 48, and 120 h) after feeding upon distinct salinities of Na+ ATPase activity and Na+/K+ ATPase in the hepatopancreas of Neohelice granulata (Dana, 1851), which is a model species. The stimulation by Na+ under total inhibition of Na+/K+ ATPase activity revealed the occurrence of Na+ ATPase activity that was totally inhibited by 2 mmol·L–1 furosemide, exhibits Michaelis–Menten kinetics for ATP (apparent Km = 0.52 ± 0.16 mmol·L–1), and highest activity at around pH 7.4. In crabs acclimated to 35 psu (osmoconforming conditions), Na+ ATPase activity was highly increased (about 15-fold) (532 ± 58 nmol Pi·mg protein−1·min−1) in the hepatopancreas 48 h after feeding. In 10 psu (hyper-regulating conditions), Na+ ATPase activity decreased in the hepatopancreas 24 h after feeding (7 ± 9 nmol Pi·mg protein−1·min−1) and recovered initial values after 48 h (24 ± 35 nmol Pi·mg protein−1·min−1). Unlike Na+ ATPase, Na+/K+ ATPase activity did not change after feeding at any salinity, suggesting the specific modulation of the second sodium pump and its role in postprandial adjustments in the hepatopancreas.
Fil: Méndez, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Caruso Neves, C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description No study has been done on the existence, biochemical characteristics, and modulation of K+-independent ouabain-insensitive Na+ ATPase activity (the second sodium pump) in the digestive tract of intertidal euryhaline crabs and moreover on the coexistence and modulation under distinct physiological and (or) environmental conditions of different sodium pumps. We determined the occurrence, characteristics, and responses at different times (0, 1, 24, 48, and 120 h) after feeding upon distinct salinities of Na+ ATPase activity and Na+/K+ ATPase in the hepatopancreas of Neohelice granulata (Dana, 1851), which is a model species. The stimulation by Na+ under total inhibition of Na+/K+ ATPase activity revealed the occurrence of Na+ ATPase activity that was totally inhibited by 2 mmol·L–1 furosemide, exhibits Michaelis–Menten kinetics for ATP (apparent Km = 0.52 ± 0.16 mmol·L–1), and highest activity at around pH 7.4. In crabs acclimated to 35 psu (osmoconforming conditions), Na+ ATPase activity was highly increased (about 15-fold) (532 ± 58 nmol Pi·mg protein−1·min−1) in the hepatopancreas 48 h after feeding. In 10 psu (hyper-regulating conditions), Na+ ATPase activity decreased in the hepatopancreas 24 h after feeding (7 ± 9 nmol Pi·mg protein−1·min−1) and recovered initial values after 48 h (24 ± 35 nmol Pi·mg protein−1·min−1). Unlike Na+ ATPase, Na+/K+ ATPase activity did not change after feeding at any salinity, suggesting the specific modulation of the second sodium pump and its role in postprandial adjustments in the hepatopancreas.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92754
Méndez, Eugenia; Caruso Neves, C.; Lopez Mañanes, Alejandra Antonia; Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 96; 6; 5-1-2018; 576-584
0008-4301
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92754
identifier_str_mv Méndez, Eugenia; Caruso Neves, C.; Lopez Mañanes, Alejandra Antonia; Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: Biochemical characteristics and differential modulation after feeding; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 96; 6; 5-1-2018; 576-584
0008-4301
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/cjz-2017-0128
info:eu-repo/semantics/altIdentifier/doi/10.1139/cjz-2017-0128
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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