Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period

Autores
Imperiale, Belén Rocío; Di Giulio, Ángela Beatríz; Mancino, María Belén; Zumárraga, Martín José; Morcillo, Nora Susana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug‑resistance (DR), multi‑DR and extensively DR (MDR/XDR‑TB), and rifampicin resistance (RIF‑R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF‑R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug‑susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro‑method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR‑TB prevalence of approximately 20% of the isolated strains, while M/XDR‑TB‑and particularly RIF‑R‑affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives’ cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR‑TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR‑TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Di Giulio, Ángela Beatríz. Provincia de Buenos Aires. Hospital "Petrona V. de Cordero"; Argentina
Fil: Mancino, María Belén. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular; Argentina
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Materia
DRUG-RESISTANCE
GENOTYPING
MUTATIONS
TUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106008
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/106008
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ PeriodImperiale, Belén RocíoDi Giulio, Ángela BeatrízMancino, María BelénZumárraga, Martín JoséMorcillo, Nora SusanaDRUG-RESISTANCEGENOTYPINGMUTATIONSTUBERCULOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug‑resistance (DR), multi‑DR and extensively DR (MDR/XDR‑TB), and rifampicin resistance (RIF‑R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF‑R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug‑susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro‑method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR‑TB prevalence of approximately 20% of the isolated strains, while M/XDR‑TB‑and particularly RIF‑R‑affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives’ cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR‑TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR‑TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Di Giulio, Ángela Beatríz. Provincia de Buenos Aires. Hospital "Petrona V. de Cordero"; ArgentinaFil: Mancino, María Belén. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Zumárraga, Martín José. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular; ArgentinaFil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaWolters Kluwer2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106008Imperiale, Belén Rocío; Di Giulio, Ángela Beatríz; Mancino, María Belén; Zumárraga, Martín José; Morcillo, Nora Susana; Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period; Wolters Kluwer; International Journal of mycobacteriology; 8; 3; 9-2019; 223-2282212-5531CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_94_19info:eu-repo/semantics/altIdentifier/url/http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=3;spage=223;epage=228;aulast=Imperialeinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:10Zoai:ri.conicet.gov.ar:11336/106008instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:10.374CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
title Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
spellingShingle Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
Imperiale, Belén Rocío
DRUG-RESISTANCE
GENOTYPING
MUTATIONS
TUBERCULOSIS
title_short Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
title_full Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
title_fullStr Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
title_full_unstemmed Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
title_sort Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period
dc.creator.none.fl_str_mv Imperiale, Belén Rocío
Di Giulio, Ángela Beatríz
Mancino, María Belén
Zumárraga, Martín José
Morcillo, Nora Susana
author Imperiale, Belén Rocío
author_facet Imperiale, Belén Rocío
Di Giulio, Ángela Beatríz
Mancino, María Belén
Zumárraga, Martín José
Morcillo, Nora Susana
author_role author
author2 Di Giulio, Ángela Beatríz
Mancino, María Belén
Zumárraga, Martín José
Morcillo, Nora Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv DRUG-RESISTANCE
GENOTYPING
MUTATIONS
TUBERCULOSIS
topic DRUG-RESISTANCE
GENOTYPING
MUTATIONS
TUBERCULOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug‑resistance (DR), multi‑DR and extensively DR (MDR/XDR‑TB), and rifampicin resistance (RIF‑R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF‑R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug‑susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro‑method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR‑TB prevalence of approximately 20% of the isolated strains, while M/XDR‑TB‑and particularly RIF‑R‑affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives’ cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR‑TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR‑TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Di Giulio, Ángela Beatríz. Provincia de Buenos Aires. Hospital "Petrona V. de Cordero"; Argentina
Fil: Mancino, María Belén. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular; Argentina
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
description Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug‑resistance (DR), multi‑DR and extensively DR (MDR/XDR‑TB), and rifampicin resistance (RIF‑R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF‑R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug‑susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro‑method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR‑TB prevalence of approximately 20% of the isolated strains, while M/XDR‑TB‑and particularly RIF‑R‑affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives’ cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR‑TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR‑TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106008
Imperiale, Belén Rocío; Di Giulio, Ángela Beatríz; Mancino, María Belén; Zumárraga, Martín José; Morcillo, Nora Susana; Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period; Wolters Kluwer; International Journal of mycobacteriology; 8; 3; 9-2019; 223-228
2212-5531
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106008
identifier_str_mv Imperiale, Belén Rocío; Di Giulio, Ángela Beatríz; Mancino, María Belén; Zumárraga, Martín José; Morcillo, Nora Susana; Surveillance and Characterization of Drug‑Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years’ Period; Wolters Kluwer; International Journal of mycobacteriology; 8; 3; 9-2019; 223-228
2212-5531
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_94_19
info:eu-repo/semantics/altIdentifier/url/http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=3;spage=223;epage=228;aulast=Imperiale
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer
publisher.none.fl_str_mv Wolters Kluwer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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