Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period

Autores
Imperiale, Belen Rocio; Di Giulio, Angela Beatríz; Mancino, María Belén; Zumarraga, Martin Jose; Morcillo, Nora
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug-resistance (DR), multi-DR and extensively DR (MDR/XDR-TB), and rifampicin resistance (RIF-R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF-R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug-susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro-method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR-TB prevalence of approximately 20% of the isolated strains, while M/XDR-TB-and particularly RIF-R-affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives' cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR-TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR-TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.
Instituto de Biotecnología
Fil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; Argentina
Fil: Mancino, María Belén. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fuente
International journal of mycobacteriology 8 (3 ): 223-228. (12-Sep-2019)
Materia
Drug Resistance
Genotypes
Mutation
Resistencia a Medicamentos
Genotipos
Mutación
Tuberculosis
Mycobacterium Tuberculosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6246
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6246
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spelling Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ periodImperiale, Belen RocioDi Giulio, Angela BeatrízMancino, María BelénZumarraga, Martin JoseMorcillo, NoraDrug ResistanceGenotypesMutationResistencia a MedicamentosGenotiposMutaciónTuberculosisMycobacterium TuberculosisBackground: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug-resistance (DR), multi-DR and extensively DR (MDR/XDR-TB), and rifampicin resistance (RIF-R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF-R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug-susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro-method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR-TB prevalence of approximately 20% of the isolated strains, while M/XDR-TB-and particularly RIF-R-affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives' cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR-TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR-TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.Instituto de BiotecnologíaFil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; ArgentinaFil: Mancino, María Belén. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; ArgentinaWolters Kluwer2019-10-30T17:45:48Z2019-10-30T17:45:48Z2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://www.ijmyco.org/text.asp?2019/8/3/223/266497http://hdl.handle.net/20.500.12123/62462212-5531https://doi.org/10.4103/ijmy.ijmy_94_19International journal of mycobacteriology 8 (3 ): 223-228. (12-Sep-2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:07Zoai:localhost:20.500.12123/6246instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:07.644INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
title Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
spellingShingle Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
Imperiale, Belen Rocio
Drug Resistance
Genotypes
Mutation
Resistencia a Medicamentos
Genotipos
Mutación
Tuberculosis
Mycobacterium Tuberculosis
title_short Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
title_full Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
title_fullStr Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
title_full_unstemmed Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
title_sort Surveillance and characterization of drug‑resistant mycobacterium tuberculosis isolated in a reference hospital from Argentina during 8 years’ period
dc.creator.none.fl_str_mv Imperiale, Belen Rocio
Di Giulio, Angela Beatríz
Mancino, María Belén
Zumarraga, Martin Jose
Morcillo, Nora
author Imperiale, Belen Rocio
author_facet Imperiale, Belen Rocio
Di Giulio, Angela Beatríz
Mancino, María Belén
Zumarraga, Martin Jose
Morcillo, Nora
author_role author
author2 Di Giulio, Angela Beatríz
Mancino, María Belén
Zumarraga, Martin Jose
Morcillo, Nora
author2_role author
author
author
author
dc.subject.none.fl_str_mv Drug Resistance
Genotypes
Mutation
Resistencia a Medicamentos
Genotipos
Mutación
Tuberculosis
Mycobacterium Tuberculosis
topic Drug Resistance
Genotypes
Mutation
Resistencia a Medicamentos
Genotipos
Mutación
Tuberculosis
Mycobacterium Tuberculosis
dc.description.none.fl_txt_mv Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug-resistance (DR), multi-DR and extensively DR (MDR/XDR-TB), and rifampicin resistance (RIF-R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF-R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug-susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro-method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR-TB prevalence of approximately 20% of the isolated strains, while M/XDR-TB-and particularly RIF-R-affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives' cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR-TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR-TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.
Instituto de Biotecnología
Fil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; Argentina
Fil: Mancino, María Belén. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
description Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug-resistance (DR), multi-DR and extensively DR (MDR/XDR-TB), and rifampicin resistance (RIF-R) burden as a part of the local TB diagnosis (June 2010–August 2018); to detect the mutations associated to isoniazid (INH) and RIF-R and their geographical distribution; and to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl–Neelsen stain and cultured on specific media. Drug-susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro-method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR-TB prevalence of approximately 20% of the isolated strains, while M/XDR-TB-and particularly RIF-R-affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives' cases with the infrequent rpoB S522 L/Q mutation. The fact that most DR/MDR-TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR-TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-30T17:45:48Z
2019-10-30T17:45:48Z
2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.ijmyco.org/text.asp?2019/8/3/223/266497
http://hdl.handle.net/20.500.12123/6246
2212-5531
https://doi.org/10.4103/ijmy.ijmy_94_19
url http://www.ijmyco.org/text.asp?2019/8/3/223/266497
http://hdl.handle.net/20.500.12123/6246
https://doi.org/10.4103/ijmy.ijmy_94_19
identifier_str_mv 2212-5531
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer
publisher.none.fl_str_mv Wolters Kluwer
dc.source.none.fl_str_mv International journal of mycobacteriology 8 (3 ): 223-228. (12-Sep-2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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