Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
- Autores
- Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; Cataldi, Ángel Adrián
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
Fil: Morcillo, Nora S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Di Giulio, Angela. Hospital Petrona V. de Cordero. San Fernando; Argentina
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Takiff, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina - Materia
-
Drug-Resistance
Tuberculosis
Fitness
Household Contacts
Family Transmission - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/35740
Ver los metadatos del registro completo
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Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contactsMorcillo, Nora S.Imperiale, Belén RocíoDi Giulio, AngelaZumárraga, Martín JoséTakiff, HowardCataldi, Ángel AdriánDrug-ResistanceTuberculosisFitnessHousehold ContactsFamily Transmissionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.Fil: Morcillo, Nora S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Di Giulio, Angela. Hospital Petrona V. de Cordero. San Fernando; ArgentinaFil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Takiff, Howard. Instituto Venezolano de Investigaciones Científicas; VenezuelaFil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaChurchill Livingstone2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35740Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; et al.; Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts; Churchill Livingstone; Tuberculosis (Edinb); 94; 6; 6-2014; 672-6771472-9792CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979214203908info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tube.2014.08.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:46Zoai:ri.conicet.gov.ar:11336/35740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:46.415CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
title |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
spellingShingle |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts Morcillo, Nora S. Drug-Resistance Tuberculosis Fitness Household Contacts Family Transmission |
title_short |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
title_full |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
title_fullStr |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
title_full_unstemmed |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
title_sort |
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
dc.creator.none.fl_str_mv |
Morcillo, Nora S. Imperiale, Belén Rocío Di Giulio, Angela Zumárraga, Martín José Takiff, Howard Cataldi, Ángel Adrián |
author |
Morcillo, Nora S. |
author_facet |
Morcillo, Nora S. Imperiale, Belén Rocío Di Giulio, Angela Zumárraga, Martín José Takiff, Howard Cataldi, Ángel Adrián |
author_role |
author |
author2 |
Imperiale, Belén Rocío Di Giulio, Angela Zumárraga, Martín José Takiff, Howard Cataldi, Ángel Adrián |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Drug-Resistance Tuberculosis Fitness Household Contacts Family Transmission |
topic |
Drug-Resistance Tuberculosis Fitness Household Contacts Family Transmission |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts. Fil: Morcillo, Nora S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina Fil: Di Giulio, Angela. Hospital Petrona V. de Cordero. San Fernando; Argentina Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Takiff, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina |
description |
There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/35740 Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; et al.; Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts; Churchill Livingstone; Tuberculosis (Edinb); 94; 6; 6-2014; 672-677 1472-9792 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/35740 |
identifier_str_mv |
Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; et al.; Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts; Churchill Livingstone; Tuberculosis (Edinb); 94; 6; 6-2014; 672-677 1472-9792 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979214203908 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tube.2014.08.003 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Churchill Livingstone |
publisher.none.fl_str_mv |
Churchill Livingstone |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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12.982451 |