Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts

Autores
Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; Cataldi, Ángel Adrián
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
Fil: Morcillo, Nora S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Di Giulio, Angela. Hospital Petrona V. de Cordero. San Fernando; Argentina
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Takiff, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Materia
Drug-Resistance
Tuberculosis
Fitness
Household Contacts
Family Transmission
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35740
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/35740
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contactsMorcillo, Nora S.Imperiale, Belén RocíoDi Giulio, AngelaZumárraga, Martín JoséTakiff, HowardCataldi, Ángel AdriánDrug-ResistanceTuberculosisFitnessHousehold ContactsFamily Transmissionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.Fil: Morcillo, Nora S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Di Giulio, Angela. Hospital Petrona V. de Cordero. San Fernando; ArgentinaFil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Takiff, Howard. Instituto Venezolano de Investigaciones Científicas; VenezuelaFil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaChurchill Livingstone2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35740Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; et al.; Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts; Churchill Livingstone; Tuberculosis (Edinb); 94; 6; 6-2014; 672-6771472-9792CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979214203908info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tube.2014.08.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:46Zoai:ri.conicet.gov.ar:11336/35740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:46.415CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
spellingShingle Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
Morcillo, Nora S.
Drug-Resistance
Tuberculosis
Fitness
Household Contacts
Family Transmission
title_short Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_full Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_fullStr Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_full_unstemmed Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_sort Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
dc.creator.none.fl_str_mv Morcillo, Nora S.
Imperiale, Belén Rocío
Di Giulio, Angela
Zumárraga, Martín José
Takiff, Howard
Cataldi, Ángel Adrián
author Morcillo, Nora S.
author_facet Morcillo, Nora S.
Imperiale, Belén Rocío
Di Giulio, Angela
Zumárraga, Martín José
Takiff, Howard
Cataldi, Ángel Adrián
author_role author
author2 Imperiale, Belén Rocío
Di Giulio, Angela
Zumárraga, Martín José
Takiff, Howard
Cataldi, Ángel Adrián
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Drug-Resistance
Tuberculosis
Fitness
Household Contacts
Family Transmission
topic Drug-Resistance
Tuberculosis
Fitness
Household Contacts
Family Transmission
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
Fil: Morcillo, Nora S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Di Giulio, Angela. Hospital Petrona V. de Cordero. San Fernando; Argentina
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Takiff, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
description There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35740
Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; et al.; Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts; Churchill Livingstone; Tuberculosis (Edinb); 94; 6; 6-2014; 672-677
1472-9792
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35740
identifier_str_mv Morcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; et al.; Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts; Churchill Livingstone; Tuberculosis (Edinb); 94; 6; 6-2014; 672-677
1472-9792
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979214203908
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tube.2014.08.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Churchill Livingstone
publisher.none.fl_str_mv Churchill Livingstone
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781244208054272
score 12.982451

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