β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening

Autores
Martinez, Gustavo Adolfo; Chaves, Alicia Raquel; Civello, Pedro Marcos
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of β-xylosidase and the expression of a β-xylosidase gene in strawberry fruit. We have cloned a cDNA fragment encoding a putative β-xylosidase (FaXyl1) from a cDNA library obtained from ripe strawberry fruit. The analysis of the deduced amino acid sequence revealed that FaXyl1 is closely related to other β-xylosidases from higher plants. The expression of FaXyl1 was strongly associated to the receptacle tissue although a low expression level was detected in achenes and ovaries. The accumulation of FaXyl1 mRNA is ripening-related, starting in white fruit, reaching the maximum at 25–50% red fruit and decreasing thereafter. The total β-xylosidase enzyme activity was detected in all ripening stages with the maximum in 25–50% red fruit. The low activity level detected in immature stages, where no expression of FaXyl1 was found, suggests the presence of other β-xylosidases-like genes. Both the expression of FaXyl1 and the total β-xylosidase activity were down regulated by auxins, as occurs for most of the ripening-related processes in strawberry fruit. A putative role of FaXyl1 and β-xylosidase is discussed.
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
FRUIT RIPENING
STRAWBERRY
XYLOSIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148747
Acceder
id CONICETDig_75b006f5d5a8d37bdd9cfd75b91dc82e
oai_identifier_str oai:ri.conicet.gov.ar:11336/148747
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripeningMartinez, Gustavo AdolfoChaves, Alicia RaquelCivello, Pedro MarcosFRUIT RIPENINGSTRAWBERRYXYLOSIDASEhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of β-xylosidase and the expression of a β-xylosidase gene in strawberry fruit. We have cloned a cDNA fragment encoding a putative β-xylosidase (FaXyl1) from a cDNA library obtained from ripe strawberry fruit. The analysis of the deduced amino acid sequence revealed that FaXyl1 is closely related to other β-xylosidases from higher plants. The expression of FaXyl1 was strongly associated to the receptacle tissue although a low expression level was detected in achenes and ovaries. The accumulation of FaXyl1 mRNA is ripening-related, starting in white fruit, reaching the maximum at 25–50% red fruit and decreasing thereafter. The total β-xylosidase enzyme activity was detected in all ripening stages with the maximum in 25–50% red fruit. The low activity level detected in immature stages, where no expression of FaXyl1 was found, suggests the presence of other β-xylosidases-like genes. Both the expression of FaXyl1 and the total β-xylosidase activity were down regulated by auxins, as occurs for most of the ripening-related processes in strawberry fruit. A putative role of FaXyl1 and β-xylosidase is discussed.Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaElsevier France-Editions Scientifiques Medicales Elsevier2004-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148747Martinez, Gustavo Adolfo; Chaves, Alicia Raquel; Civello, Pedro Marcos; β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 42; 2; 2-2004; 89-960981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2003.12.001info:eu-repo/semantics/altIdentifier/url/https://bit.ly/3Ez9ri0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:31Zoai:ri.conicet.gov.ar:11336/148747instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:32.18CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
title β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
spellingShingle β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
Martinez, Gustavo Adolfo
FRUIT RIPENING
STRAWBERRY
XYLOSIDASE
title_short β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
title_full β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
title_fullStr β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
title_full_unstemmed β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
title_sort β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
dc.creator.none.fl_str_mv Martinez, Gustavo Adolfo
Chaves, Alicia Raquel
Civello, Pedro Marcos
author Martinez, Gustavo Adolfo
author_facet Martinez, Gustavo Adolfo
Chaves, Alicia Raquel
Civello, Pedro Marcos
author_role author
author2 Chaves, Alicia Raquel
Civello, Pedro Marcos
author2_role author
author
dc.subject.none.fl_str_mv FRUIT RIPENING
STRAWBERRY
XYLOSIDASE
topic FRUIT RIPENING
STRAWBERRY
XYLOSIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of β-xylosidase and the expression of a β-xylosidase gene in strawberry fruit. We have cloned a cDNA fragment encoding a putative β-xylosidase (FaXyl1) from a cDNA library obtained from ripe strawberry fruit. The analysis of the deduced amino acid sequence revealed that FaXyl1 is closely related to other β-xylosidases from higher plants. The expression of FaXyl1 was strongly associated to the receptacle tissue although a low expression level was detected in achenes and ovaries. The accumulation of FaXyl1 mRNA is ripening-related, starting in white fruit, reaching the maximum at 25–50% red fruit and decreasing thereafter. The total β-xylosidase enzyme activity was detected in all ripening stages with the maximum in 25–50% red fruit. The low activity level detected in immature stages, where no expression of FaXyl1 was found, suggests the presence of other β-xylosidases-like genes. Both the expression of FaXyl1 and the total β-xylosidase activity were down regulated by auxins, as occurs for most of the ripening-related processes in strawberry fruit. A putative role of FaXyl1 and β-xylosidase is discussed.
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of β-xylosidase and the expression of a β-xylosidase gene in strawberry fruit. We have cloned a cDNA fragment encoding a putative β-xylosidase (FaXyl1) from a cDNA library obtained from ripe strawberry fruit. The analysis of the deduced amino acid sequence revealed that FaXyl1 is closely related to other β-xylosidases from higher plants. The expression of FaXyl1 was strongly associated to the receptacle tissue although a low expression level was detected in achenes and ovaries. The accumulation of FaXyl1 mRNA is ripening-related, starting in white fruit, reaching the maximum at 25–50% red fruit and decreasing thereafter. The total β-xylosidase enzyme activity was detected in all ripening stages with the maximum in 25–50% red fruit. The low activity level detected in immature stages, where no expression of FaXyl1 was found, suggests the presence of other β-xylosidases-like genes. Both the expression of FaXyl1 and the total β-xylosidase activity were down regulated by auxins, as occurs for most of the ripening-related processes in strawberry fruit. A putative role of FaXyl1 and β-xylosidase is discussed.
publishDate 2004
dc.date.none.fl_str_mv 2004-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148747
Martinez, Gustavo Adolfo; Chaves, Alicia Raquel; Civello, Pedro Marcos; β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 42; 2; 2-2004; 89-96
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148747
identifier_str_mv Martinez, Gustavo Adolfo; Chaves, Alicia Raquel; Civello, Pedro Marcos; β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 42; 2; 2-2004; 89-96
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2003.12.001
info:eu-repo/semantics/altIdentifier/url/https://bit.ly/3Ez9ri0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-Editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-Editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613954059370496
score 13.070432

Publicaciones similares