Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit

Autores
Bustamante, Claudia Anabel; Civello, Pedro Marcos; Martinez, Gustavo Adolfo
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Expression and activity of cell wall modifying enzymes involved in fruit softening may be regulated by hormones and/or other signal molecules. In strawberry, FaXyl1 encodes for a fruit-specific b-xylosidase probably associated to hemicellulose degradation. In this work, we have isolated and analysed the promoter region of FaXyl1 gene. Analysis of the sequence revealed the presence of cis-acting elements associated with hormone, light and stress-related responses. Several treatments were done on fruit in order to prove the responsiveness of FaXyl1 to plant growth regulators related to the regulatory elements identified in the promoter region (abscisic acid, auxins and gibberellins) and others associated with the ripening process (ethylene and nitric oxide). The effect of each treatment on FaXyl1 expression, the corresponding protein levels and the b-xylosidase activity was evaluated. ABA (abscisic acid) stimulated FaXyl1 expression and protein levels. In contrast, expression levels of FaXyl1 gene decreased after treatments with NAA (naphthalene acetic acid), GA3 (gibberellic acid) and ethephon, an ethylenegenerating compound. SNP (sodium nitroprusside), a NO donor, did not affect FaXyl1 mRNA and protein levels. The effect of 1-MCP (1-methylcyclopropene), an ethylene perception inhibitor, on FaXyl1 expression was consistent with the effect observed with ethephon. b-xylosidase activity was down regulated by NAA, whereas GA3, ABA, ethephon, 1-MCP and SNP had no effect on it.
Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Materia
b-XYLOSIDASE
CELL WALL
PLANT GROWTH REGULATORS
RIPENING
SOFTENING
STRAWBERRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262690
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/262690
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruitBustamante, Claudia AnabelCivello, Pedro MarcosMartinez, Gustavo Adolfob-XYLOSIDASECELL WALLPLANT GROWTH REGULATORSRIPENINGSOFTENINGSTRAWBERRYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Expression and activity of cell wall modifying enzymes involved in fruit softening may be regulated by hormones and/or other signal molecules. In strawberry, FaXyl1 encodes for a fruit-specific b-xylosidase probably associated to hemicellulose degradation. In this work, we have isolated and analysed the promoter region of FaXyl1 gene. Analysis of the sequence revealed the presence of cis-acting elements associated with hormone, light and stress-related responses. Several treatments were done on fruit in order to prove the responsiveness of FaXyl1 to plant growth regulators related to the regulatory elements identified in the promoter region (abscisic acid, auxins and gibberellins) and others associated with the ripening process (ethylene and nitric oxide). The effect of each treatment on FaXyl1 expression, the corresponding protein levels and the b-xylosidase activity was evaluated. ABA (abscisic acid) stimulated FaXyl1 expression and protein levels. In contrast, expression levels of FaXyl1 gene decreased after treatments with NAA (naphthalene acetic acid), GA3 (gibberellic acid) and ethephon, an ethylenegenerating compound. SNP (sodium nitroprusside), a NO donor, did not affect FaXyl1 mRNA and protein levels. The effect of 1-MCP (1-methylcyclopropene), an ethylene perception inhibitor, on FaXyl1 expression was consistent with the effect observed with ethephon. b-xylosidase activity was down regulated by NAA, whereas GA3, ABA, ethephon, 1-MCP and SNP had no effect on it.Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaElsevier Ireland2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262690Bustamante, Claudia Anabel; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit; Elsevier Ireland; Plant Science; 177; 1; 7-2009; 49-560168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945209000983info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2009.03.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:13Zoai:ri.conicet.gov.ar:11336/262690instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:13.706CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
title Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
spellingShingle Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
Bustamante, Claudia Anabel
b-XYLOSIDASE
CELL WALL
PLANT GROWTH REGULATORS
RIPENING
SOFTENING
STRAWBERRY
title_short Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
title_full Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
title_fullStr Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
title_full_unstemmed Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
title_sort Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
dc.creator.none.fl_str_mv Bustamante, Claudia Anabel
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author Bustamante, Claudia Anabel
author_facet Bustamante, Claudia Anabel
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author_role author
author2 Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author2_role author
author
dc.subject.none.fl_str_mv b-XYLOSIDASE
CELL WALL
PLANT GROWTH REGULATORS
RIPENING
SOFTENING
STRAWBERRY
topic b-XYLOSIDASE
CELL WALL
PLANT GROWTH REGULATORS
RIPENING
SOFTENING
STRAWBERRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Expression and activity of cell wall modifying enzymes involved in fruit softening may be regulated by hormones and/or other signal molecules. In strawberry, FaXyl1 encodes for a fruit-specific b-xylosidase probably associated to hemicellulose degradation. In this work, we have isolated and analysed the promoter region of FaXyl1 gene. Analysis of the sequence revealed the presence of cis-acting elements associated with hormone, light and stress-related responses. Several treatments were done on fruit in order to prove the responsiveness of FaXyl1 to plant growth regulators related to the regulatory elements identified in the promoter region (abscisic acid, auxins and gibberellins) and others associated with the ripening process (ethylene and nitric oxide). The effect of each treatment on FaXyl1 expression, the corresponding protein levels and the b-xylosidase activity was evaluated. ABA (abscisic acid) stimulated FaXyl1 expression and protein levels. In contrast, expression levels of FaXyl1 gene decreased after treatments with NAA (naphthalene acetic acid), GA3 (gibberellic acid) and ethephon, an ethylenegenerating compound. SNP (sodium nitroprusside), a NO donor, did not affect FaXyl1 mRNA and protein levels. The effect of 1-MCP (1-methylcyclopropene), an ethylene perception inhibitor, on FaXyl1 expression was consistent with the effect observed with ethephon. b-xylosidase activity was down regulated by NAA, whereas GA3, ABA, ethephon, 1-MCP and SNP had no effect on it.
Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
description Expression and activity of cell wall modifying enzymes involved in fruit softening may be regulated by hormones and/or other signal molecules. In strawberry, FaXyl1 encodes for a fruit-specific b-xylosidase probably associated to hemicellulose degradation. In this work, we have isolated and analysed the promoter region of FaXyl1 gene. Analysis of the sequence revealed the presence of cis-acting elements associated with hormone, light and stress-related responses. Several treatments were done on fruit in order to prove the responsiveness of FaXyl1 to plant growth regulators related to the regulatory elements identified in the promoter region (abscisic acid, auxins and gibberellins) and others associated with the ripening process (ethylene and nitric oxide). The effect of each treatment on FaXyl1 expression, the corresponding protein levels and the b-xylosidase activity was evaluated. ABA (abscisic acid) stimulated FaXyl1 expression and protein levels. In contrast, expression levels of FaXyl1 gene decreased after treatments with NAA (naphthalene acetic acid), GA3 (gibberellic acid) and ethephon, an ethylenegenerating compound. SNP (sodium nitroprusside), a NO donor, did not affect FaXyl1 mRNA and protein levels. The effect of 1-MCP (1-methylcyclopropene), an ethylene perception inhibitor, on FaXyl1 expression was consistent with the effect observed with ethephon. b-xylosidase activity was down regulated by NAA, whereas GA3, ABA, ethephon, 1-MCP and SNP had no effect on it.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262690
Bustamante, Claudia Anabel; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit; Elsevier Ireland; Plant Science; 177; 1; 7-2009; 49-56
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262690
identifier_str_mv Bustamante, Claudia Anabel; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit; Elsevier Ireland; Plant Science; 177; 1; 7-2009; 49-56
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945209000983
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2009.03.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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