Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol

Autores
Coleta, D. J.; Missana, Liliana Raquel; Martins, T.; García, L.; Farez, N.; De Glee, T.; Dias, M.; Moreno Garcia, Maria Florencia; Mardegan Issa, J. P.; Pereira,M .; Feldman, Sara
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Synthetic three-dimensional scaffolds for application in bone tissue regeneration should present architecture similar to bone extracellular matrix and provide suitable microenvironment for cell adhesion, proliferation and differentiation. The aims of this work were: 1) to make a structural characterization of a three-dimensional hybrid scaffold (MH) formed as a hybrid porous containing chitosan, polivynil alcohol and bioactive glass by SEM (Scanning Electron Microscopy), 2) to study in vitro potential cytotoxicity from the scaffolds assessing cell metabolic activity (MTT assay) and 3) to study in vivo rabbit response to MH implant into 5.1mm femoral injury, using treated and control animals (n=5). Results: SEM showed well defined MH net with open and interconnected pores, homogeneously organized with 64-234 µm range. MH had no cytotoxic effect detected by MTT. No clinical and biochemical (hemogram, total proteins and transaminases) differences were detected among groups. Histological studies showed a bi-layer tissue at matrix-interface, inner fibrous and outer bony tissues. The intra-scaffold area presented mineralized surface by cementing laminar-granular deposits, some of them resembling osseous formation with osteocytes-like and lining-like cells. However, inside the matrix, leukocyte hemorrhaged exudate with many macrophages, as well as cells debris were observed. MH can be considered a promising bone tissue biomaterial though longer studies should be carried to determine the macrophages cells fate.
Fil: Coleta, D. J.. Universidad Nacional de Rosario; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martins, T.. No especifíca;
Fil: García, L.. Universidad Nacional de Rosario; Argentina
Fil: Farez, N.. Universidad Nacional de Rosario; Argentina
Fil: De Glee, T.. Universidad Nacional de Rosario; Argentina
Fil: Dias, M.. Universidad Nacional de Rosario; Argentina
Fil: Moreno Garcia, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Mardegan Issa, J. P.. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira,M .. Universidade Federal de Minas Gerais; Brasil
Fil: Feldman, Sara. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
XXXIV Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral
Ciudad Autónoma de Buenos Aires
Argentina
Asociación Argentina de Osteología y Metabolismo Mineral
Materia
ENGENEERING
TISUE
BONE
REGENERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/188589
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/188589
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcoholColeta, D. J.Missana, Liliana RaquelMartins, T.García, L.Farez, N.De Glee, T.Dias, M.Moreno Garcia, Maria FlorenciaMardegan Issa, J. P.Pereira,M .Feldman, SaraENGENEERINGTISUEBONEREGENERATIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Synthetic three-dimensional scaffolds for application in bone tissue regeneration should present architecture similar to bone extracellular matrix and provide suitable microenvironment for cell adhesion, proliferation and differentiation. The aims of this work were: 1) to make a structural characterization of a three-dimensional hybrid scaffold (MH) formed as a hybrid porous containing chitosan, polivynil alcohol and bioactive glass by SEM (Scanning Electron Microscopy), 2) to study in vitro potential cytotoxicity from the scaffolds assessing cell metabolic activity (MTT assay) and 3) to study in vivo rabbit response to MH implant into 5.1mm femoral injury, using treated and control animals (n=5). Results: SEM showed well defined MH net with open and interconnected pores, homogeneously organized with 64-234 µm range. MH had no cytotoxic effect detected by MTT. No clinical and biochemical (hemogram, total proteins and transaminases) differences were detected among groups. Histological studies showed a bi-layer tissue at matrix-interface, inner fibrous and outer bony tissues. The intra-scaffold area presented mineralized surface by cementing laminar-granular deposits, some of them resembling osseous formation with osteocytes-like and lining-like cells. However, inside the matrix, leukocyte hemorrhaged exudate with many macrophages, as well as cells debris were observed. MH can be considered a promising bone tissue biomaterial though longer studies should be carried to determine the macrophages cells fate.Fil: Coleta, D. J.. Universidad Nacional de Rosario; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martins, T.. No especifíca;Fil: García, L.. Universidad Nacional de Rosario; ArgentinaFil: Farez, N.. Universidad Nacional de Rosario; ArgentinaFil: De Glee, T.. Universidad Nacional de Rosario; ArgentinaFil: Dias, M.. Universidad Nacional de Rosario; ArgentinaFil: Moreno Garcia, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Mardegan Issa, J. P.. Universidade Federal de Minas Gerais; BrasilFil: Pereira,M .. Universidade Federal de Minas Gerais; BrasilFil: Feldman, Sara. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaXXXIV Reunión Anual de la Asociación Argentina de Osteología y Metabolismo MineralCiudad Autónoma de Buenos AiresArgentinaAsociación Argentina de Osteología y Metabolismo MineralAsociación Argentina de Osteología y Metabolismo Mineral2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/188589Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol; XXXIV Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; Ciudad Autónoma de Buenos Aires; Argentina; 2017; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aaomm.org.ar/wp-content/uploads/2020/07/Comunicaciones-libres-2017.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:24Zoai:ri.conicet.gov.ar:11336/188589instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:25.23CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
title Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
spellingShingle Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
Coleta, D. J.
ENGENEERING
TISUE
BONE
REGENERATION
title_short Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
title_full Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
title_fullStr Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
title_full_unstemmed Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
title_sort Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol
dc.creator.none.fl_str_mv Coleta, D. J.
Missana, Liliana Raquel
Martins, T.
García, L.
Farez, N.
De Glee, T.
Dias, M.
Moreno Garcia, Maria Florencia
Mardegan Issa, J. P.
Pereira,M .
Feldman, Sara
author Coleta, D. J.
author_facet Coleta, D. J.
Missana, Liliana Raquel
Martins, T.
García, L.
Farez, N.
De Glee, T.
Dias, M.
Moreno Garcia, Maria Florencia
Mardegan Issa, J. P.
Pereira,M .
Feldman, Sara
author_role author
author2 Missana, Liliana Raquel
Martins, T.
García, L.
Farez, N.
De Glee, T.
Dias, M.
Moreno Garcia, Maria Florencia
Mardegan Issa, J. P.
Pereira,M .
Feldman, Sara
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENGENEERING
TISUE
BONE
REGENERATION
topic ENGENEERING
TISUE
BONE
REGENERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Synthetic three-dimensional scaffolds for application in bone tissue regeneration should present architecture similar to bone extracellular matrix and provide suitable microenvironment for cell adhesion, proliferation and differentiation. The aims of this work were: 1) to make a structural characterization of a three-dimensional hybrid scaffold (MH) formed as a hybrid porous containing chitosan, polivynil alcohol and bioactive glass by SEM (Scanning Electron Microscopy), 2) to study in vitro potential cytotoxicity from the scaffolds assessing cell metabolic activity (MTT assay) and 3) to study in vivo rabbit response to MH implant into 5.1mm femoral injury, using treated and control animals (n=5). Results: SEM showed well defined MH net with open and interconnected pores, homogeneously organized with 64-234 µm range. MH had no cytotoxic effect detected by MTT. No clinical and biochemical (hemogram, total proteins and transaminases) differences were detected among groups. Histological studies showed a bi-layer tissue at matrix-interface, inner fibrous and outer bony tissues. The intra-scaffold area presented mineralized surface by cementing laminar-granular deposits, some of them resembling osseous formation with osteocytes-like and lining-like cells. However, inside the matrix, leukocyte hemorrhaged exudate with many macrophages, as well as cells debris were observed. MH can be considered a promising bone tissue biomaterial though longer studies should be carried to determine the macrophages cells fate.
Fil: Coleta, D. J.. Universidad Nacional de Rosario; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martins, T.. No especifíca;
Fil: García, L.. Universidad Nacional de Rosario; Argentina
Fil: Farez, N.. Universidad Nacional de Rosario; Argentina
Fil: De Glee, T.. Universidad Nacional de Rosario; Argentina
Fil: Dias, M.. Universidad Nacional de Rosario; Argentina
Fil: Moreno Garcia, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Mardegan Issa, J. P.. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira,M .. Universidade Federal de Minas Gerais; Brasil
Fil: Feldman, Sara. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
XXXIV Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral
Ciudad Autónoma de Buenos Aires
Argentina
Asociación Argentina de Osteología y Metabolismo Mineral
description Synthetic three-dimensional scaffolds for application in bone tissue regeneration should present architecture similar to bone extracellular matrix and provide suitable microenvironment for cell adhesion, proliferation and differentiation. The aims of this work were: 1) to make a structural characterization of a three-dimensional hybrid scaffold (MH) formed as a hybrid porous containing chitosan, polivynil alcohol and bioactive glass by SEM (Scanning Electron Microscopy), 2) to study in vitro potential cytotoxicity from the scaffolds assessing cell metabolic activity (MTT assay) and 3) to study in vivo rabbit response to MH implant into 5.1mm femoral injury, using treated and control animals (n=5). Results: SEM showed well defined MH net with open and interconnected pores, homogeneously organized with 64-234 µm range. MH had no cytotoxic effect detected by MTT. No clinical and biochemical (hemogram, total proteins and transaminases) differences were detected among groups. Histological studies showed a bi-layer tissue at matrix-interface, inner fibrous and outer bony tissues. The intra-scaffold area presented mineralized surface by cementing laminar-granular deposits, some of them resembling osseous formation with osteocytes-like and lining-like cells. However, inside the matrix, leukocyte hemorrhaged exudate with many macrophages, as well as cells debris were observed. MH can be considered a promising bone tissue biomaterial though longer studies should be carried to determine the macrophages cells fate.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/188589
Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol; XXXIV Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; Ciudad Autónoma de Buenos Aires; Argentina; 2017; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/188589
identifier_str_mv Ingeniería de tejidos óseos: Estudios in vitro e in vivo con matrices híbridas de vidrio bioactivo, quitosano y polivinilalcohol; XXXIV Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; Ciudad Autónoma de Buenos Aires; Argentina; 2017; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aaomm.org.ar/wp-content/uploads/2020/07/Comunicaciones-libres-2017.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Asociación Argentina de Osteología y Metabolismo Mineral
publisher.none.fl_str_mv Asociación Argentina de Osteología y Metabolismo Mineral
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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