Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene

Autores
Monferran, Magdalena Victoria; Pesce, Silvia Fabiana; Cazenave, Jimena; Wunderlin, Daniel Alberto
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report changes in activities of detoxification and antioxidant enzymes as well as lipid peroxidation levels in liver, gills, and brain of Jenynsia multidentata exposed to 1,2- and 1,4-dichlorobenzene (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L−1 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L−1. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GST activity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish. The detoxification system was activated at lower concentrations of 1,2-DCB than 1,4-DCB. Antioxidant response is activated in liver at low DCB concentrations, followed by a drop at highest levels. We also found activation of the antioxidant system in gills and brain of exposed fish. On the other hand, we did not observe changes in TBARS concentrations in liver or gills of exposed fish with respect to controls, but in brain of fish exposed to 1,2-DCB (≥0.5 mg L−1) and 1,4-DCB (5 mg L−1). Responses of both detoxification and antioxidant systems of J. multidentata suggest that 1,2-DCB is more toxic than 1,4-DCB to this specie. To the extent of our knowledge, this is the first report of oxidative stress induced by DCBs in fish. Our results evidence that the brain is the organ most severely affected by the oxidative stress caused by DCBs.
Fil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Pesce, Silvia Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cazenave, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Diversidad Animal I; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
Dcbs
Fish
Antioxidant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27050
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/27050
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzeneMonferran, Magdalena VictoriaPesce, Silvia FabianaCazenave, JimenaWunderlin, Daniel AlbertoDcbsFishAntioxidanthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We report changes in activities of detoxification and antioxidant enzymes as well as lipid peroxidation levels in liver, gills, and brain of Jenynsia multidentata exposed to 1,2- and 1,4-dichlorobenzene (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L−1 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L−1. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GST activity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish. The detoxification system was activated at lower concentrations of 1,2-DCB than 1,4-DCB. Antioxidant response is activated in liver at low DCB concentrations, followed by a drop at highest levels. We also found activation of the antioxidant system in gills and brain of exposed fish. On the other hand, we did not observe changes in TBARS concentrations in liver or gills of exposed fish with respect to controls, but in brain of fish exposed to 1,2-DCB (≥0.5 mg L−1) and 1,4-DCB (5 mg L−1). Responses of both detoxification and antioxidant systems of J. multidentata suggest that 1,2-DCB is more toxic than 1,4-DCB to this specie. To the extent of our knowledge, this is the first report of oxidative stress induced by DCBs in fish. Our results evidence that the brain is the organ most severely affected by the oxidative stress caused by DCBs.Fil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Pesce, Silvia Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cazenave, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Diversidad Animal I; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaWiley2008-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27050Monferran, Magdalena Victoria; Pesce, Silvia Fabiana; Cazenave, Jimena; Wunderlin, Daniel Alberto; Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene; Wiley; Environmental Toxicology; 23; 2; 1-2008; 184-1921520-40811522-7278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/tox.20326info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.20326/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:29Zoai:ri.conicet.gov.ar:11336/27050instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:29.445CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
title Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
spellingShingle Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
Monferran, Magdalena Victoria
Dcbs
Fish
Antioxidant
title_short Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
title_full Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
title_fullStr Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
title_full_unstemmed Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
title_sort Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene
dc.creator.none.fl_str_mv Monferran, Magdalena Victoria
Pesce, Silvia Fabiana
Cazenave, Jimena
Wunderlin, Daniel Alberto
author Monferran, Magdalena Victoria
author_facet Monferran, Magdalena Victoria
Pesce, Silvia Fabiana
Cazenave, Jimena
Wunderlin, Daniel Alberto
author_role author
author2 Pesce, Silvia Fabiana
Cazenave, Jimena
Wunderlin, Daniel Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Dcbs
Fish
Antioxidant
topic Dcbs
Fish
Antioxidant
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report changes in activities of detoxification and antioxidant enzymes as well as lipid peroxidation levels in liver, gills, and brain of Jenynsia multidentata exposed to 1,2- and 1,4-dichlorobenzene (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L−1 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L−1. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GST activity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish. The detoxification system was activated at lower concentrations of 1,2-DCB than 1,4-DCB. Antioxidant response is activated in liver at low DCB concentrations, followed by a drop at highest levels. We also found activation of the antioxidant system in gills and brain of exposed fish. On the other hand, we did not observe changes in TBARS concentrations in liver or gills of exposed fish with respect to controls, but in brain of fish exposed to 1,2-DCB (≥0.5 mg L−1) and 1,4-DCB (5 mg L−1). Responses of both detoxification and antioxidant systems of J. multidentata suggest that 1,2-DCB is more toxic than 1,4-DCB to this specie. To the extent of our knowledge, this is the first report of oxidative stress induced by DCBs in fish. Our results evidence that the brain is the organ most severely affected by the oxidative stress caused by DCBs.
Fil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Pesce, Silvia Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cazenave, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Diversidad Animal I; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description We report changes in activities of detoxification and antioxidant enzymes as well as lipid peroxidation levels in liver, gills, and brain of Jenynsia multidentata exposed to 1,2- and 1,4-dichlorobenzene (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L−1 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L−1. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GST activity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish. The detoxification system was activated at lower concentrations of 1,2-DCB than 1,4-DCB. Antioxidant response is activated in liver at low DCB concentrations, followed by a drop at highest levels. We also found activation of the antioxidant system in gills and brain of exposed fish. On the other hand, we did not observe changes in TBARS concentrations in liver or gills of exposed fish with respect to controls, but in brain of fish exposed to 1,2-DCB (≥0.5 mg L−1) and 1,4-DCB (5 mg L−1). Responses of both detoxification and antioxidant systems of J. multidentata suggest that 1,2-DCB is more toxic than 1,4-DCB to this specie. To the extent of our knowledge, this is the first report of oxidative stress induced by DCBs in fish. Our results evidence that the brain is the organ most severely affected by the oxidative stress caused by DCBs.
publishDate 2008
dc.date.none.fl_str_mv 2008-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27050
Monferran, Magdalena Victoria; Pesce, Silvia Fabiana; Cazenave, Jimena; Wunderlin, Daniel Alberto; Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene; Wiley; Environmental Toxicology; 23; 2; 1-2008; 184-192
1520-4081
1522-7278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27050
identifier_str_mv Monferran, Magdalena Victoria; Pesce, Silvia Fabiana; Cazenave, Jimena; Wunderlin, Daniel Alberto; Detoxification and antioxidant responses in diverse organs of Jenynsia multidentata experimentally exposed to 1,2- and 1,4-dichlorobenzene; Wiley; Environmental Toxicology; 23; 2; 1-2008; 184-192
1520-4081
1522-7278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/tox.20326
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.20326/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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