Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures

Autores
Bonansea, Rocio Ines; Wunderlin, Daniel Alberto; Amé, María Valeria
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pesticides cypermethrin (CYP) and chlorpyrifos (CPF) were found together in water bodies located in agricultural and urban areas. However, the impact to non-target biota from exposure to mixtures has received little attention. In the current study, we evaluated changes in swimming behavior and cholinesterase enzymes activity in Jenynsia multidentata, to investigate the possible effects of these insecticides individually and in mixtures. Moreover, differences between technical and commercial mixtures of the pesticides were evaluated. Females of J. multidentata were exposed over 96 h to CYP (0.04 and 0.4 µg L-1), CPF (0.4 and 4 µg L-1), individually and in a technical and commercial mixtures. Swimming behavior was recorded after 24 h and 96 h of exposure. Also, we measured cholinesterase enzymes activity in brain and muscle after 96 h of exposure. Exposure to CYP increased the exploratory activity of J. multidentata in the upper area of the aquarium. Fish exposed to CPF (4 µg L-1) showed a decrease in swimming activity and an increase in the time spent at the bottom of the aquarium. Interestingly, fish exposed to the technical and commercial mixture of CYP and CPF displayed a different behavior based on the concentration of exposure. Low concentration of pesticides elicited an increase in J. multidentata swimming activity with preference for the upper area of the aquarium, and high concentrations caused decrease in swimming activity with preference for the bottom area of the aquarium. Based on the response of cholinesterase enzymes, acetylcholinesterase in muscle was more sensitive to exposure to CYP, CPF and their mixtures than in brain. A decrease in swimming behavior correlates significantly with the inhibition of acetylcholinesterase activity in muscle of J. multidentata exposed to high concentrations of pesticides. These results draw attention to the need of more studies on the potential ecotoxicological impact of pesticides and its mixtures at environmental relevant concentrations.
Fil: Bonansea, Rocio Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
Fish Behavior
Acetylcholinesterase Activity
Mixtures
Insecticides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46281
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46281
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixturesBonansea, Rocio InesWunderlin, Daniel AlbertoAmé, María ValeriaFish BehaviorAcetylcholinesterase ActivityMixturesInsecticideshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The pesticides cypermethrin (CYP) and chlorpyrifos (CPF) were found together in water bodies located in agricultural and urban areas. However, the impact to non-target biota from exposure to mixtures has received little attention. In the current study, we evaluated changes in swimming behavior and cholinesterase enzymes activity in Jenynsia multidentata, to investigate the possible effects of these insecticides individually and in mixtures. Moreover, differences between technical and commercial mixtures of the pesticides were evaluated. Females of J. multidentata were exposed over 96 h to CYP (0.04 and 0.4 µg L-1), CPF (0.4 and 4 µg L-1), individually and in a technical and commercial mixtures. Swimming behavior was recorded after 24 h and 96 h of exposure. Also, we measured cholinesterase enzymes activity in brain and muscle after 96 h of exposure. Exposure to CYP increased the exploratory activity of J. multidentata in the upper area of the aquarium. Fish exposed to CPF (4 µg L-1) showed a decrease in swimming activity and an increase in the time spent at the bottom of the aquarium. Interestingly, fish exposed to the technical and commercial mixture of CYP and CPF displayed a different behavior based on the concentration of exposure. Low concentration of pesticides elicited an increase in J. multidentata swimming activity with preference for the upper area of the aquarium, and high concentrations caused decrease in swimming activity with preference for the bottom area of the aquarium. Based on the response of cholinesterase enzymes, acetylcholinesterase in muscle was more sensitive to exposure to CYP, CPF and their mixtures than in brain. A decrease in swimming behavior correlates significantly with the inhibition of acetylcholinesterase activity in muscle of J. multidentata exposed to high concentrations of pesticides. These results draw attention to the need of more studies on the potential ecotoxicological impact of pesticides and its mixtures at environmental relevant concentrations.Fil: Bonansea, Rocio Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaAcademic Press Inc Elsevier Science2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46281Bonansea, Rocio Ines; Wunderlin, Daniel Alberto; Amé, María Valeria; Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 129; 6-2016; 311-3190147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2016.03.043info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651316301038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:27Zoai:ri.conicet.gov.ar:11336/46281instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:27.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
title Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
spellingShingle Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
Bonansea, Rocio Ines
Fish Behavior
Acetylcholinesterase Activity
Mixtures
Insecticides
title_short Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
title_full Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
title_fullStr Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
title_full_unstemmed Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
title_sort Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures
dc.creator.none.fl_str_mv Bonansea, Rocio Ines
Wunderlin, Daniel Alberto
Amé, María Valeria
author Bonansea, Rocio Ines
author_facet Bonansea, Rocio Ines
Wunderlin, Daniel Alberto
Amé, María Valeria
author_role author
author2 Wunderlin, Daniel Alberto
Amé, María Valeria
author2_role author
author
dc.subject.none.fl_str_mv Fish Behavior
Acetylcholinesterase Activity
Mixtures
Insecticides
topic Fish Behavior
Acetylcholinesterase Activity
Mixtures
Insecticides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The pesticides cypermethrin (CYP) and chlorpyrifos (CPF) were found together in water bodies located in agricultural and urban areas. However, the impact to non-target biota from exposure to mixtures has received little attention. In the current study, we evaluated changes in swimming behavior and cholinesterase enzymes activity in Jenynsia multidentata, to investigate the possible effects of these insecticides individually and in mixtures. Moreover, differences between technical and commercial mixtures of the pesticides were evaluated. Females of J. multidentata were exposed over 96 h to CYP (0.04 and 0.4 µg L-1), CPF (0.4 and 4 µg L-1), individually and in a technical and commercial mixtures. Swimming behavior was recorded after 24 h and 96 h of exposure. Also, we measured cholinesterase enzymes activity in brain and muscle after 96 h of exposure. Exposure to CYP increased the exploratory activity of J. multidentata in the upper area of the aquarium. Fish exposed to CPF (4 µg L-1) showed a decrease in swimming activity and an increase in the time spent at the bottom of the aquarium. Interestingly, fish exposed to the technical and commercial mixture of CYP and CPF displayed a different behavior based on the concentration of exposure. Low concentration of pesticides elicited an increase in J. multidentata swimming activity with preference for the upper area of the aquarium, and high concentrations caused decrease in swimming activity with preference for the bottom area of the aquarium. Based on the response of cholinesterase enzymes, acetylcholinesterase in muscle was more sensitive to exposure to CYP, CPF and their mixtures than in brain. A decrease in swimming behavior correlates significantly with the inhibition of acetylcholinesterase activity in muscle of J. multidentata exposed to high concentrations of pesticides. These results draw attention to the need of more studies on the potential ecotoxicological impact of pesticides and its mixtures at environmental relevant concentrations.
Fil: Bonansea, Rocio Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description The pesticides cypermethrin (CYP) and chlorpyrifos (CPF) were found together in water bodies located in agricultural and urban areas. However, the impact to non-target biota from exposure to mixtures has received little attention. In the current study, we evaluated changes in swimming behavior and cholinesterase enzymes activity in Jenynsia multidentata, to investigate the possible effects of these insecticides individually and in mixtures. Moreover, differences between technical and commercial mixtures of the pesticides were evaluated. Females of J. multidentata were exposed over 96 h to CYP (0.04 and 0.4 µg L-1), CPF (0.4 and 4 µg L-1), individually and in a technical and commercial mixtures. Swimming behavior was recorded after 24 h and 96 h of exposure. Also, we measured cholinesterase enzymes activity in brain and muscle after 96 h of exposure. Exposure to CYP increased the exploratory activity of J. multidentata in the upper area of the aquarium. Fish exposed to CPF (4 µg L-1) showed a decrease in swimming activity and an increase in the time spent at the bottom of the aquarium. Interestingly, fish exposed to the technical and commercial mixture of CYP and CPF displayed a different behavior based on the concentration of exposure. Low concentration of pesticides elicited an increase in J. multidentata swimming activity with preference for the upper area of the aquarium, and high concentrations caused decrease in swimming activity with preference for the bottom area of the aquarium. Based on the response of cholinesterase enzymes, acetylcholinesterase in muscle was more sensitive to exposure to CYP, CPF and their mixtures than in brain. A decrease in swimming behavior correlates significantly with the inhibition of acetylcholinesterase activity in muscle of J. multidentata exposed to high concentrations of pesticides. These results draw attention to the need of more studies on the potential ecotoxicological impact of pesticides and its mixtures at environmental relevant concentrations.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46281
Bonansea, Rocio Ines; Wunderlin, Daniel Alberto; Amé, María Valeria; Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 129; 6-2016; 311-319
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46281
identifier_str_mv Bonansea, Rocio Ines; Wunderlin, Daniel Alberto; Amé, María Valeria; Behavioral swimming effects and acetylcholinesterase activitychanges in Jenynsia multidentata exposed to chlorpyrifos and cypermethrin individually and in mixtures; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 129; 6-2016; 311-319
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2016.03.043
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651316301038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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