Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole

Autores
Andrioli, Nancy Beatriz; Sabatini, Sebastian Eduardo; Mudry, Marta Dolores; Ríos de Molina, María del Carmen
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The toxicity of metronidazole (MTZ) in meristematic and elongation zones of Allium cepa roots was analyzed for 30h of exposition. Toxic effects were evaluated by lipid peroxidation (content of thiobarbituric-reactive substances [TBARS]), reduced glutathione (GSH) levels, ascorbate acid and dehydroascorbate acid content, and enzymatic activities of superoxide dismutase and catalase. The root zones showed differentiated susceptibility to MTZ. In the elongation zone, MTZ induced an increase of TBARS content and a significant rise in GSH levels, whereas in the meristematic zone, lipid peroxidation was not observed and all antioxidant defense parameters analyzed were significantly increased. These results indicate that MTZ exposure induced oxidative stress in A. cepa roots, and that the antioxidant defenses in the meristematic zone are more efficient compared with the elongation zone, which is probably related to higher oxidative metabolism of meristematic tissue.
Fil: Andrioli, Nancy Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Ríos de Molina, María del Carmen. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
ALLIUM CEPA
ANTIOXIDANT DEFENSES
METRONIDAZOLE
OXIDATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195446
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195446
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazoleAndrioli, Nancy BeatrizSabatini, Sebastian EduardoMudry, Marta DoloresRíos de Molina, María del CarmenALLIUM CEPAANTIOXIDANT DEFENSESMETRONIDAZOLEOXIDATIVE STRESShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The toxicity of metronidazole (MTZ) in meristematic and elongation zones of Allium cepa roots was analyzed for 30h of exposition. Toxic effects were evaluated by lipid peroxidation (content of thiobarbituric-reactive substances [TBARS]), reduced glutathione (GSH) levels, ascorbate acid and dehydroascorbate acid content, and enzymatic activities of superoxide dismutase and catalase. The root zones showed differentiated susceptibility to MTZ. In the elongation zone, MTZ induced an increase of TBARS content and a significant rise in GSH levels, whereas in the meristematic zone, lipid peroxidation was not observed and all antioxidant defense parameters analyzed were significantly increased. These results indicate that MTZ exposure induced oxidative stress in A. cepa roots, and that the antioxidant defenses in the meristematic zone are more efficient compared with the elongation zone, which is probably related to higher oxidative metabolism of meristematic tissue.Fil: Andrioli, Nancy Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Ríos de Molina, María del Carmen. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaSociety of Environmental Toxicology and Chemistry2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195446Andrioli, Nancy Beatriz; Sabatini, Sebastian Eduardo; Mudry, Marta Dolores; Ríos de Molina, María del Carmen; Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 31; 5; 3-2012; 968-9720730-7268CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://setac.onlinelibrary.wiley.com/doi/abs/10.1002/etc.1799info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.1799info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:44:19Zoai:ri.conicet.gov.ar:11336/195446instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:44:19.357CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
title Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
spellingShingle Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
Andrioli, Nancy Beatriz
ALLIUM CEPA
ANTIOXIDANT DEFENSES
METRONIDAZOLE
OXIDATIVE STRESS
title_short Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
title_full Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
title_fullStr Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
title_full_unstemmed Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
title_sort Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole
dc.creator.none.fl_str_mv Andrioli, Nancy Beatriz
Sabatini, Sebastian Eduardo
Mudry, Marta Dolores
Ríos de Molina, María del Carmen
author Andrioli, Nancy Beatriz
author_facet Andrioli, Nancy Beatriz
Sabatini, Sebastian Eduardo
Mudry, Marta Dolores
Ríos de Molina, María del Carmen
author_role author
author2 Sabatini, Sebastian Eduardo
Mudry, Marta Dolores
Ríos de Molina, María del Carmen
author2_role author
author
author
dc.subject.none.fl_str_mv ALLIUM CEPA
ANTIOXIDANT DEFENSES
METRONIDAZOLE
OXIDATIVE STRESS
topic ALLIUM CEPA
ANTIOXIDANT DEFENSES
METRONIDAZOLE
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The toxicity of metronidazole (MTZ) in meristematic and elongation zones of Allium cepa roots was analyzed for 30h of exposition. Toxic effects were evaluated by lipid peroxidation (content of thiobarbituric-reactive substances [TBARS]), reduced glutathione (GSH) levels, ascorbate acid and dehydroascorbate acid content, and enzymatic activities of superoxide dismutase and catalase. The root zones showed differentiated susceptibility to MTZ. In the elongation zone, MTZ induced an increase of TBARS content and a significant rise in GSH levels, whereas in the meristematic zone, lipid peroxidation was not observed and all antioxidant defense parameters analyzed were significantly increased. These results indicate that MTZ exposure induced oxidative stress in A. cepa roots, and that the antioxidant defenses in the meristematic zone are more efficient compared with the elongation zone, which is probably related to higher oxidative metabolism of meristematic tissue.
Fil: Andrioli, Nancy Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Ríos de Molina, María del Carmen. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description The toxicity of metronidazole (MTZ) in meristematic and elongation zones of Allium cepa roots was analyzed for 30h of exposition. Toxic effects were evaluated by lipid peroxidation (content of thiobarbituric-reactive substances [TBARS]), reduced glutathione (GSH) levels, ascorbate acid and dehydroascorbate acid content, and enzymatic activities of superoxide dismutase and catalase. The root zones showed differentiated susceptibility to MTZ. In the elongation zone, MTZ induced an increase of TBARS content and a significant rise in GSH levels, whereas in the meristematic zone, lipid peroxidation was not observed and all antioxidant defense parameters analyzed were significantly increased. These results indicate that MTZ exposure induced oxidative stress in A. cepa roots, and that the antioxidant defenses in the meristematic zone are more efficient compared with the elongation zone, which is probably related to higher oxidative metabolism of meristematic tissue.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195446
Andrioli, Nancy Beatriz; Sabatini, Sebastian Eduardo; Mudry, Marta Dolores; Ríos de Molina, María del Carmen; Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 31; 5; 3-2012; 968-972
0730-7268
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195446
identifier_str_mv Andrioli, Nancy Beatriz; Sabatini, Sebastian Eduardo; Mudry, Marta Dolores; Ríos de Molina, María del Carmen; Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 31; 5; 3-2012; 968-972
0730-7268
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://setac.onlinelibrary.wiley.com/doi/abs/10.1002/etc.1799
info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.1799
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083546060423168
score 13.22299

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