Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity

Autores
Monserrat, José María; Garcia, M. L.; Ventura Lima, J.; Gonzalez, Mariana; Ballesteros, Maria Laura; Miglioranza, Karina Silvia Beatriz; Amé, María Valeria; Wunderlin, Daniel Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antioxidants like lipoic acid (LA) are known to trigger augmented antioxidant and phase II and III responses. This study aimed to evaluate the effect of LA in P-glycoprotein (Pgp) expression, glutathione-S-transferase (GST) activity, total antioxidant competence, levels of lipid peroxides (TBARS) and accumulation of the organochlorine insecticide endosulfan (Endo: α-, β-isomers and sulfate metabolite) in different organs of the fish Jenynsia multidentata. One hundred and twenty females (1.55 ± 0.07 g) were fed during 8 days with (n = 60) or without (n = 60) a LA enriched ration (6000 mg/kg). Four experimental groups were defined: −LA/−Endo; +LA/−Endo; −LA/+Endo; and +LA/+Endo. Endo groups were exposed during 24 h to 1.4 μg of insecticide/L. Results showed that only LA induced a significant increment in liver Pgp expression. GST activity was augmented in liver after exposure to LA or Endo. TBARS levels were lowered in liver and gills after LA pre-treatment. Total antioxidant capacity was lowered in liver of Endo exposed fish, a result that was reversed by LA pre-treatment. It is concluded that LA induced the expected effects in terms of Pgp expression, GST activity and reduced TBARS levels although favored α-Endo accumulation in brain. However, the Endo metabolism to the more persistent endosulfan sulfate was not facilitated by LA pre-treatment.
Fil: Monserrat, José María. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; Brasil
Fil: Garcia, M. L.. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; Brasil
Fil: Ventura Lima, J.. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; Brasil
Fil: Gonzalez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Miglioranza, Karina Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Amé, María Valeria. Universidad Nacional de Córdoba; Argentina
Fil: Wunderlin, Daniel Alberto. Universidad Nacional de Córdoba; Argentina
Materia
Lipoic acid
Endosulfan
Pgp expression
Antioxidant defenses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8010
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8010
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicityMonserrat, José MaríaGarcia, M. L.Ventura Lima, J.Gonzalez, MarianaBallesteros, Maria LauraMiglioranza, Karina Silvia BeatrizAmé, María ValeriaWunderlin, Daniel AlbertoLipoic acidEndosulfanPgp expressionAntioxidant defenseshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Antioxidants like lipoic acid (LA) are known to trigger augmented antioxidant and phase II and III responses. This study aimed to evaluate the effect of LA in P-glycoprotein (Pgp) expression, glutathione-S-transferase (GST) activity, total antioxidant competence, levels of lipid peroxides (TBARS) and accumulation of the organochlorine insecticide endosulfan (Endo: α-, β-isomers and sulfate metabolite) in different organs of the fish <em>Jenynsia multidentata</em>. One hundred and twenty females (1.55 ± 0.07 g) were fed during 8 days with (<em>n</em> = 60) or without (<em>n</em> = 60) a LA enriched ration (6000 mg/kg). Four experimental groups were defined: −LA/−Endo; +LA/−Endo; −LA/+Endo; and +LA/+Endo. Endo groups were exposed during 24 h to 1.4 μg of insecticide/L. Results showed that only LA induced a significant increment in liver Pgp expression. GST activity was augmented in liver after exposure to LA or Endo. TBARS levels were lowered in liver and gills after LA pre-treatment. Total antioxidant capacity was lowered in liver of Endo exposed fish, a result that was reversed by LA pre-treatment. It is concluded that LA induced the expected effects in terms of Pgp expression, GST activity and reduced TBARS levels although favored α-Endo accumulation in brain. However, the Endo metabolism to the more persistent endosulfan sulfate was not facilitated by LA pre-treatment.Fil: Monserrat, José María. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; BrasilFil: Garcia, M. L.. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; BrasilFil: Ventura Lima, J.. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; BrasilFil: Gonzalez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Miglioranza, Karina Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Amé, María Valeria. Universidad Nacional de Córdoba; ArgentinaFil: Wunderlin, Daniel Alberto. Universidad Nacional de Córdoba; ArgentinaElsevier2013-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8010Monserrat, José María; Garcia, M. L.; Ventura Lima, J.; Gonzalez, Mariana; Ballesteros, Maria Laura; et al.; Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity; Elsevier; Pesticide Biochemistry And Physiology; 108; 1-11-2013; 8-150048-3575enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.pestbp.2013.10.009info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048357513001727info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:05Zoai:ri.conicet.gov.ar:11336/8010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:05.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
title Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
spellingShingle Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
Monserrat, José María
Lipoic acid
Endosulfan
Pgp expression
Antioxidant defenses
title_short Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
title_full Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
title_fullStr Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
title_full_unstemmed Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
title_sort Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
dc.creator.none.fl_str_mv Monserrat, José María
Garcia, M. L.
Ventura Lima, J.
Gonzalez, Mariana
Ballesteros, Maria Laura
Miglioranza, Karina Silvia Beatriz
Amé, María Valeria
Wunderlin, Daniel Alberto
author Monserrat, José María
author_facet Monserrat, José María
Garcia, M. L.
Ventura Lima, J.
Gonzalez, Mariana
Ballesteros, Maria Laura
Miglioranza, Karina Silvia Beatriz
Amé, María Valeria
Wunderlin, Daniel Alberto
author_role author
author2 Garcia, M. L.
Ventura Lima, J.
Gonzalez, Mariana
Ballesteros, Maria Laura
Miglioranza, Karina Silvia Beatriz
Amé, María Valeria
Wunderlin, Daniel Alberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lipoic acid
Endosulfan
Pgp expression
Antioxidant defenses
topic Lipoic acid
Endosulfan
Pgp expression
Antioxidant defenses
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Antioxidants like lipoic acid (LA) are known to trigger augmented antioxidant and phase II and III responses. This study aimed to evaluate the effect of LA in P-glycoprotein (Pgp) expression, glutathione-S-transferase (GST) activity, total antioxidant competence, levels of lipid peroxides (TBARS) and accumulation of the organochlorine insecticide endosulfan (Endo: α-, β-isomers and sulfate metabolite) in different organs of the fish <em>Jenynsia multidentata</em>. One hundred and twenty females (1.55 ± 0.07 g) were fed during 8 days with (<em>n</em> = 60) or without (<em>n</em> = 60) a LA enriched ration (6000 mg/kg). Four experimental groups were defined: −LA/−Endo; +LA/−Endo; −LA/+Endo; and +LA/+Endo. Endo groups were exposed during 24 h to 1.4 μg of insecticide/L. Results showed that only LA induced a significant increment in liver Pgp expression. GST activity was augmented in liver after exposure to LA or Endo. TBARS levels were lowered in liver and gills after LA pre-treatment. Total antioxidant capacity was lowered in liver of Endo exposed fish, a result that was reversed by LA pre-treatment. It is concluded that LA induced the expected effects in terms of Pgp expression, GST activity and reduced TBARS levels although favored α-Endo accumulation in brain. However, the Endo metabolism to the more persistent endosulfan sulfate was not facilitated by LA pre-treatment.
Fil: Monserrat, José María. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; Brasil
Fil: Garcia, M. L.. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; Brasil
Fil: Ventura Lima, J.. Universidade Federal do Rio Grande do Norte; Brasil. Instituto de Ciências Biológicas; Brasil
Fil: Gonzalez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Miglioranza, Karina Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Amé, María Valeria. Universidad Nacional de Córdoba; Argentina
Fil: Wunderlin, Daniel Alberto. Universidad Nacional de Córdoba; Argentina
description Antioxidants like lipoic acid (LA) are known to trigger augmented antioxidant and phase II and III responses. This study aimed to evaluate the effect of LA in P-glycoprotein (Pgp) expression, glutathione-S-transferase (GST) activity, total antioxidant competence, levels of lipid peroxides (TBARS) and accumulation of the organochlorine insecticide endosulfan (Endo: α-, β-isomers and sulfate metabolite) in different organs of the fish <em>Jenynsia multidentata</em>. One hundred and twenty females (1.55 ± 0.07 g) were fed during 8 days with (<em>n</em> = 60) or without (<em>n</em> = 60) a LA enriched ration (6000 mg/kg). Four experimental groups were defined: −LA/−Endo; +LA/−Endo; −LA/+Endo; and +LA/+Endo. Endo groups were exposed during 24 h to 1.4 μg of insecticide/L. Results showed that only LA induced a significant increment in liver Pgp expression. GST activity was augmented in liver after exposure to LA or Endo. TBARS levels were lowered in liver and gills after LA pre-treatment. Total antioxidant capacity was lowered in liver of Endo exposed fish, a result that was reversed by LA pre-treatment. It is concluded that LA induced the expected effects in terms of Pgp expression, GST activity and reduced TBARS levels although favored α-Endo accumulation in brain. However, the Endo metabolism to the more persistent endosulfan sulfate was not facilitated by LA pre-treatment.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8010
Monserrat, José María; Garcia, M. L.; Ventura Lima, J.; Gonzalez, Mariana; Ballesteros, Maria Laura; et al.; Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity; Elsevier; Pesticide Biochemistry And Physiology; 108; 1-11-2013; 8-15
0048-3575
url http://hdl.handle.net/11336/8010
identifier_str_mv Monserrat, José María; Garcia, M. L.; Ventura Lima, J.; Gonzalez, Mariana; Ballesteros, Maria Laura; et al.; Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity; Elsevier; Pesticide Biochemistry And Physiology; 108; 1-11-2013; 8-15
0048-3575
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.pestbp.2013.10.009
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048357513001727
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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