Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina

Autores
Perez, Maria Florencia; Saona Acuña, Luis Alberto; Farias, Maria Eugenia; Poehlein, Anja; Meinhardt, Friedhelm; Daniel, Rolf; Dib, Julian Rafael
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diamante Lake located at 4589 m.a.s.l. in the Andean Puna constitutes an extreme environment. It is exposed to multiple extreme conditions such as an unusually high concentration of arsenic (over 300 mg L-1) and low oxygen pressure. Microorganisms thriving in the lake display specific genotypes facilitating survival, which include at least a multitude of plasmid-encoded resistance traits. Hence, the genetic information provided by the plasmids essentially contributes to understand adaptation to different stressors. Plasmids from cultivable organisms have already been analyzed to the sequence level, the impact of the entire plasmid-borne genetic information on such microbial ecosystem is not known.This study aims at assessing the plasmidome from Diamante Lake, which facilitates identification of potential hosts and prediction of gene functions. The analysis revealed a large fraction of previously unknown DNA sequences, the majority of which encoded putative proteins of unknown function. Remarkably, functions related to the oxidative stress response, DNA repair, as well as arsenic- and antibiotic resistances were annotated. Additionally, all necessary capacities related to plasmid replication, mobilization and maintenance were detected. Sequences characteristic for megaplasmids and other already known plasmid-associated genes were identified as well.The study highlights the potential of the deep-sequencing approach specifically targeting plasmid populations as it allows to evaluate the ecological impact of plasmids from (cultivable and non-cultivable) microorganisms, thereby contributing to the understanding of the distribution of resistance factors within an extremophilic microbial community.
Fil: Perez, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Saona Acuña, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Poehlein, Anja. Universität Göttingen; Alemania
Fil: Meinhardt, Friedhelm. Westfälische Wilhelms Universität; Alemania
Fil: Daniel, Rolf. Universität Göttingen; Alemania
Fil: Dib, Julian Rafael. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
PLASMIDOME
PLASMID
PUNA ARGENTINA
EXTREME EVIRONMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158463
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/158463
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, ArgentinaPerez, Maria FlorenciaSaona Acuña, Luis AlbertoFarias, Maria EugeniaPoehlein, AnjaMeinhardt, FriedhelmDaniel, RolfDib, Julian RafaelPLASMIDOMEPLASMIDPUNA ARGENTINAEXTREME EVIRONMENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diamante Lake located at 4589 m.a.s.l. in the Andean Puna constitutes an extreme environment. It is exposed to multiple extreme conditions such as an unusually high concentration of arsenic (over 300 mg L-1) and low oxygen pressure. Microorganisms thriving in the lake display specific genotypes facilitating survival, which include at least a multitude of plasmid-encoded resistance traits. Hence, the genetic information provided by the plasmids essentially contributes to understand adaptation to different stressors. Plasmids from cultivable organisms have already been analyzed to the sequence level, the impact of the entire plasmid-borne genetic information on such microbial ecosystem is not known.This study aims at assessing the plasmidome from Diamante Lake, which facilitates identification of potential hosts and prediction of gene functions. The analysis revealed a large fraction of previously unknown DNA sequences, the majority of which encoded putative proteins of unknown function. Remarkably, functions related to the oxidative stress response, DNA repair, as well as arsenic- and antibiotic resistances were annotated. Additionally, all necessary capacities related to plasmid replication, mobilization and maintenance were detected. Sequences characteristic for megaplasmids and other already known plasmid-associated genes were identified as well.The study highlights the potential of the deep-sequencing approach specifically targeting plasmid populations as it allows to evaluate the ecological impact of plasmids from (cultivable and non-cultivable) microorganisms, thereby contributing to the understanding of the distribution of resistance factors within an extremophilic microbial community.Fil: Perez, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Saona Acuña, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Poehlein, Anja. Universität Göttingen; AlemaniaFil: Meinhardt, Friedhelm. Westfälische Wilhelms Universität; AlemaniaFil: Daniel, Rolf. Universität Göttingen; AlemaniaFil: Dib, Julian Rafael. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaNature2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158463Perez, Maria Florencia; Saona Acuña, Luis Alberto; Farias, Maria Eugenia; Poehlein, Anja; Meinhardt, Friedhelm; et al.; Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina; Nature; Scientific Reports; 11; 1; 11-20212045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-00753-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:57Zoai:ri.conicet.gov.ar:11336/158463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:58.08CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
title Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
spellingShingle Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
Perez, Maria Florencia
PLASMIDOME
PLASMID
PUNA ARGENTINA
EXTREME EVIRONMENT
title_short Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
title_full Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
title_fullStr Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
title_full_unstemmed Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
title_sort Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina
dc.creator.none.fl_str_mv Perez, Maria Florencia
Saona Acuña, Luis Alberto
Farias, Maria Eugenia
Poehlein, Anja
Meinhardt, Friedhelm
Daniel, Rolf
Dib, Julian Rafael
author Perez, Maria Florencia
author_facet Perez, Maria Florencia
Saona Acuña, Luis Alberto
Farias, Maria Eugenia
Poehlein, Anja
Meinhardt, Friedhelm
Daniel, Rolf
Dib, Julian Rafael
author_role author
author2 Saona Acuña, Luis Alberto
Farias, Maria Eugenia
Poehlein, Anja
Meinhardt, Friedhelm
Daniel, Rolf
Dib, Julian Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PLASMIDOME
PLASMID
PUNA ARGENTINA
EXTREME EVIRONMENT
topic PLASMIDOME
PLASMID
PUNA ARGENTINA
EXTREME EVIRONMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Diamante Lake located at 4589 m.a.s.l. in the Andean Puna constitutes an extreme environment. It is exposed to multiple extreme conditions such as an unusually high concentration of arsenic (over 300 mg L-1) and low oxygen pressure. Microorganisms thriving in the lake display specific genotypes facilitating survival, which include at least a multitude of plasmid-encoded resistance traits. Hence, the genetic information provided by the plasmids essentially contributes to understand adaptation to different stressors. Plasmids from cultivable organisms have already been analyzed to the sequence level, the impact of the entire plasmid-borne genetic information on such microbial ecosystem is not known.This study aims at assessing the plasmidome from Diamante Lake, which facilitates identification of potential hosts and prediction of gene functions. The analysis revealed a large fraction of previously unknown DNA sequences, the majority of which encoded putative proteins of unknown function. Remarkably, functions related to the oxidative stress response, DNA repair, as well as arsenic- and antibiotic resistances were annotated. Additionally, all necessary capacities related to plasmid replication, mobilization and maintenance were detected. Sequences characteristic for megaplasmids and other already known plasmid-associated genes were identified as well.The study highlights the potential of the deep-sequencing approach specifically targeting plasmid populations as it allows to evaluate the ecological impact of plasmids from (cultivable and non-cultivable) microorganisms, thereby contributing to the understanding of the distribution of resistance factors within an extremophilic microbial community.
Fil: Perez, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Saona Acuña, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Poehlein, Anja. Universität Göttingen; Alemania
Fil: Meinhardt, Friedhelm. Westfälische Wilhelms Universität; Alemania
Fil: Daniel, Rolf. Universität Göttingen; Alemania
Fil: Dib, Julian Rafael. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Diamante Lake located at 4589 m.a.s.l. in the Andean Puna constitutes an extreme environment. It is exposed to multiple extreme conditions such as an unusually high concentration of arsenic (over 300 mg L-1) and low oxygen pressure. Microorganisms thriving in the lake display specific genotypes facilitating survival, which include at least a multitude of plasmid-encoded resistance traits. Hence, the genetic information provided by the plasmids essentially contributes to understand adaptation to different stressors. Plasmids from cultivable organisms have already been analyzed to the sequence level, the impact of the entire plasmid-borne genetic information on such microbial ecosystem is not known.This study aims at assessing the plasmidome from Diamante Lake, which facilitates identification of potential hosts and prediction of gene functions. The analysis revealed a large fraction of previously unknown DNA sequences, the majority of which encoded putative proteins of unknown function. Remarkably, functions related to the oxidative stress response, DNA repair, as well as arsenic- and antibiotic resistances were annotated. Additionally, all necessary capacities related to plasmid replication, mobilization and maintenance were detected. Sequences characteristic for megaplasmids and other already known plasmid-associated genes were identified as well.The study highlights the potential of the deep-sequencing approach specifically targeting plasmid populations as it allows to evaluate the ecological impact of plasmids from (cultivable and non-cultivable) microorganisms, thereby contributing to the understanding of the distribution of resistance factors within an extremophilic microbial community.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158463
Perez, Maria Florencia; Saona Acuña, Luis Alberto; Farias, Maria Eugenia; Poehlein, Anja; Meinhardt, Friedhelm; et al.; Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina; Nature; Scientific Reports; 11; 1; 11-2021
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158463
identifier_str_mv Perez, Maria Florencia; Saona Acuña, Luis Alberto; Farias, Maria Eugenia; Poehlein, Anja; Meinhardt, Friedhelm; et al.; Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina; Nature; Scientific Reports; 11; 1; 11-2021
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-00753-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269829370740736
score 13.13397

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