Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation

Autores
Perez, Maria Florencia; Angelov, Angel; Übelacker, Maria; Torres Tejerizo, Gonzalo Arturo; Farias, Maria Eugenia; Liebl, Wolfgang; Dib, Julian Rafael
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Actinobacteria have frequently been reported in the Andean Puna, including strains of the genus Micrococcus. These strains demonstrate resistance to high levels of UV radiation, arsenic, and multiple antibiotics, and possess large linear plasmids. A comparative analysis of the sequences and putative functions of these plasmids was conducted. The presence of large regions with high sequence identity (exceeding 30 kb in total) in all three studied Micrococcus megaplasmids indicates a clear evolutionary link among these elements. Genes related to essential plasmid functions were primarily found within these conserved regions, while genes associated with resistance to metals and antibiotics resided in accessory regions. Moreover, the abundance of open reading frames related to transposition and recombination, along with local deviations from the average GC content, provides evidence for the mosaic nature and considerable genetic plasticity of these plasmids. This study presents evidence of a common ancestor for linear plasmids in Micrococcus and suggests that horizontal gene transfer likely occurs frequently within Andean lakes, providing the native microbial community with a beneficial gene pool to withstand extreme conditions. Additionally, the successful transfer of the linear plasmid pLMA1 by a DNase-insensitive, conjugation-type mechanism and its potential use as a genetic vector is demonstrated.
Fil: Perez, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Angelov, Angel. Universitat Technical Zu Munich; Alemania
Fil: Übelacker, Maria. Universitat Technical Zu Munich; Alemania
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Liebl, Wolfgang. Universitat Technical Zu Munich; Alemania
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
ANDEAN PUNA
EXTREME CONDITIONS
HORIZONTAL TRANSFER
LINEAR PLASMID
MICROCOCCUS
RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263179
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/263179
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by ConjugationPerez, Maria FlorenciaAngelov, AngelÜbelacker, MariaTorres Tejerizo, Gonzalo ArturoFarias, Maria EugeniaLiebl, WolfgangDib, Julian RafaelANDEAN PUNAEXTREME CONDITIONSHORIZONTAL TRANSFERLINEAR PLASMIDMICROCOCCUSRESISTANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Actinobacteria have frequently been reported in the Andean Puna, including strains of the genus Micrococcus. These strains demonstrate resistance to high levels of UV radiation, arsenic, and multiple antibiotics, and possess large linear plasmids. A comparative analysis of the sequences and putative functions of these plasmids was conducted. The presence of large regions with high sequence identity (exceeding 30 kb in total) in all three studied Micrococcus megaplasmids indicates a clear evolutionary link among these elements. Genes related to essential plasmid functions were primarily found within these conserved regions, while genes associated with resistance to metals and antibiotics resided in accessory regions. Moreover, the abundance of open reading frames related to transposition and recombination, along with local deviations from the average GC content, provides evidence for the mosaic nature and considerable genetic plasticity of these plasmids. This study presents evidence of a common ancestor for linear plasmids in Micrococcus and suggests that horizontal gene transfer likely occurs frequently within Andean lakes, providing the native microbial community with a beneficial gene pool to withstand extreme conditions. Additionally, the successful transfer of the linear plasmid pLMA1 by a DNase-insensitive, conjugation-type mechanism and its potential use as a genetic vector is demonstrated.Fil: Perez, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Angelov, Angel. Universitat Technical Zu Munich; AlemaniaFil: Übelacker, Maria. Universitat Technical Zu Munich; AlemaniaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Liebl, Wolfgang. Universitat Technical Zu Munich; AlemaniaFil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaWiley Blackwell Publishing, Inc2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263179Perez, Maria Florencia; Angelov, Angel; Übelacker, Maria; Torres Tejerizo, Gonzalo Arturo; Farias, Maria Eugenia; et al.; Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 27; 1; 1-2025; 1-151462-2912CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.70020info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.70020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:42Zoai:ri.conicet.gov.ar:11336/263179instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:43.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
title Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
spellingShingle Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
Perez, Maria Florencia
ANDEAN PUNA
EXTREME CONDITIONS
HORIZONTAL TRANSFER
LINEAR PLASMID
MICROCOCCUS
RESISTANCE
title_short Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
title_full Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
title_fullStr Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
title_full_unstemmed Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
title_sort Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
dc.creator.none.fl_str_mv Perez, Maria Florencia
Angelov, Angel
Übelacker, Maria
Torres Tejerizo, Gonzalo Arturo
Farias, Maria Eugenia
Liebl, Wolfgang
Dib, Julian Rafael
author Perez, Maria Florencia
author_facet Perez, Maria Florencia
Angelov, Angel
Übelacker, Maria
Torres Tejerizo, Gonzalo Arturo
Farias, Maria Eugenia
Liebl, Wolfgang
Dib, Julian Rafael
author_role author
author2 Angelov, Angel
Übelacker, Maria
Torres Tejerizo, Gonzalo Arturo
Farias, Maria Eugenia
Liebl, Wolfgang
Dib, Julian Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANDEAN PUNA
EXTREME CONDITIONS
HORIZONTAL TRANSFER
LINEAR PLASMID
MICROCOCCUS
RESISTANCE
topic ANDEAN PUNA
EXTREME CONDITIONS
HORIZONTAL TRANSFER
LINEAR PLASMID
MICROCOCCUS
RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Actinobacteria have frequently been reported in the Andean Puna, including strains of the genus Micrococcus. These strains demonstrate resistance to high levels of UV radiation, arsenic, and multiple antibiotics, and possess large linear plasmids. A comparative analysis of the sequences and putative functions of these plasmids was conducted. The presence of large regions with high sequence identity (exceeding 30 kb in total) in all three studied Micrococcus megaplasmids indicates a clear evolutionary link among these elements. Genes related to essential plasmid functions were primarily found within these conserved regions, while genes associated with resistance to metals and antibiotics resided in accessory regions. Moreover, the abundance of open reading frames related to transposition and recombination, along with local deviations from the average GC content, provides evidence for the mosaic nature and considerable genetic plasticity of these plasmids. This study presents evidence of a common ancestor for linear plasmids in Micrococcus and suggests that horizontal gene transfer likely occurs frequently within Andean lakes, providing the native microbial community with a beneficial gene pool to withstand extreme conditions. Additionally, the successful transfer of the linear plasmid pLMA1 by a DNase-insensitive, conjugation-type mechanism and its potential use as a genetic vector is demonstrated.
Fil: Perez, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Angelov, Angel. Universitat Technical Zu Munich; Alemania
Fil: Übelacker, Maria. Universitat Technical Zu Munich; Alemania
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Liebl, Wolfgang. Universitat Technical Zu Munich; Alemania
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Actinobacteria have frequently been reported in the Andean Puna, including strains of the genus Micrococcus. These strains demonstrate resistance to high levels of UV radiation, arsenic, and multiple antibiotics, and possess large linear plasmids. A comparative analysis of the sequences and putative functions of these plasmids was conducted. The presence of large regions with high sequence identity (exceeding 30 kb in total) in all three studied Micrococcus megaplasmids indicates a clear evolutionary link among these elements. Genes related to essential plasmid functions were primarily found within these conserved regions, while genes associated with resistance to metals and antibiotics resided in accessory regions. Moreover, the abundance of open reading frames related to transposition and recombination, along with local deviations from the average GC content, provides evidence for the mosaic nature and considerable genetic plasticity of these plasmids. This study presents evidence of a common ancestor for linear plasmids in Micrococcus and suggests that horizontal gene transfer likely occurs frequently within Andean lakes, providing the native microbial community with a beneficial gene pool to withstand extreme conditions. Additionally, the successful transfer of the linear plasmid pLMA1 by a DNase-insensitive, conjugation-type mechanism and its potential use as a genetic vector is demonstrated.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263179
Perez, Maria Florencia; Angelov, Angel; Übelacker, Maria; Torres Tejerizo, Gonzalo Arturo; Farias, Maria Eugenia; et al.; Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 27; 1; 1-2025; 1-15
1462-2912
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263179
identifier_str_mv Perez, Maria Florencia; Angelov, Angel; Übelacker, Maria; Torres Tejerizo, Gonzalo Arturo; Farias, Maria Eugenia; et al.; Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 27; 1; 1-2025; 1-15
1462-2912
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.70020
info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.70020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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