Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production

Autores
Zannier, Federico; Portero, Luciano Raúl; Ordoñez, Omar Federico; Martinez, Luciano José; Farias, Maria Eugenia; Albarracín, Virginia Helena
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High levels of arsenic present in High Altitude Andean Lakes(HAAL) ecosystems have selected arsenic-resistant microbial communities which are of novel interest to study adaptations mechanisms which can be applied in bioremediation processes. We herein performed a detailedcharacterization of the arsenic tolerance profiles and the biofilm production oftwo HAAL poly-extremophiles, Acinetobacter sp. Ver3 (Ver3) and Exiguobacterium sp. S17 (S17). Cellular adherence over glass andpolypropylene surfaces were evaluated together with the effect of increasingdoses and oxidative states of arsenic over the quality and quantity of theirbiofilm production.The arsenic tolerance outcomes showed that HAAL strains couldtolerate higher arsenic concentrations than phylogenetically related strainsbelonging to the German collection of microorganisms and cell cultures(Deutsche Sammlung von Mikroorganismen und Zellkulturen, DSMZ), whichsuggest adaptations of HAAL strains to their original environment. On the otherhand, the crystal violet method (CV) and SEM analysis showed that Ver3 andS17 were able to attach to solid surfaces and to form the biofilm. Thequantification of biofilms production in 48 hours cultures through CV shows thatVer3 yielded higher production in the treatment without arsenic cultured on aglass support, while S17 yield higher biofilm production under intermediatearsenic concentration on glass supports. Polypropylene supports had negativeeffects on the biofilm production of Ver3 and S17. SEM analysis shows that the highest biofilm yields could be associated with a larger number of attached cells as well as the development of more complex 3D multicellular structures.
Fil: Zannier, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Portero, Luciano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martinez, Luciano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
Bioremediation
Acinetobacter
Exiguobacterium
Puna Region
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82286
Acceder
id CONICETDig_ed07d43deb6515fa05e5bddb6cd8d104
oai_identifier_str oai:ri.conicet.gov.ar:11336/82286
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm productionZannier, FedericoPortero, Luciano RaúlOrdoñez, Omar FedericoMartinez, Luciano JoséFarias, Maria EugeniaAlbarracín, Virginia HelenaBioremediationAcinetobacterExiguobacteriumPuna Regionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High levels of arsenic present in High Altitude Andean Lakes(HAAL) ecosystems have selected arsenic-resistant microbial communities which are of novel interest to study adaptations mechanisms which can be applied in bioremediation processes. We herein performed a detailedcharacterization of the arsenic tolerance profiles and the biofilm production oftwo HAAL poly-extremophiles, Acinetobacter sp. Ver3 (Ver3) and Exiguobacterium sp. S17 (S17). Cellular adherence over glass andpolypropylene surfaces were evaluated together with the effect of increasingdoses and oxidative states of arsenic over the quality and quantity of theirbiofilm production.The arsenic tolerance outcomes showed that HAAL strains couldtolerate higher arsenic concentrations than phylogenetically related strainsbelonging to the German collection of microorganisms and cell cultures(Deutsche Sammlung von Mikroorganismen und Zellkulturen, DSMZ), whichsuggest adaptations of HAAL strains to their original environment. On the otherhand, the crystal violet method (CV) and SEM analysis showed that Ver3 andS17 were able to attach to solid surfaces and to form the biofilm. Thequantification of biofilms production in 48 hours cultures through CV shows thatVer3 yielded higher production in the treatment without arsenic cultured on aglass support, while S17 yield higher biofilm production under intermediatearsenic concentration on glass supports. Polypropylene supports had negativeeffects on the biofilm production of Ver3 and S17. SEM analysis shows that the highest biofilm yields could be associated with a larger number of attached cells as well as the development of more complex 3D multicellular structures.Fil: Zannier, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Portero, Luciano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martinez, Luciano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaHindawi Publishing Corporation2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82286Zannier, Federico; Portero, Luciano Raúl; Ordoñez, Omar Federico; Martinez, Luciano José; Farias, Maria Eugenia; et al.; Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production; Hindawi Publishing Corporation; BioMed Research International; 2019; 1-2019; 1-122314-6133CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2019/1231975/abs/info:eu-repo/semantics/altIdentifier/doi/10.1155/2019/1231975info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:18Zoai:ri.conicet.gov.ar:11336/82286instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:19.213CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
title Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
spellingShingle Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
Zannier, Federico
Bioremediation
Acinetobacter
Exiguobacterium
Puna Region
title_short Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
title_full Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
title_fullStr Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
title_full_unstemmed Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
title_sort Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production
dc.creator.none.fl_str_mv Zannier, Federico
Portero, Luciano Raúl
Ordoñez, Omar Federico
Martinez, Luciano José
Farias, Maria Eugenia
Albarracín, Virginia Helena
author Zannier, Federico
author_facet Zannier, Federico
Portero, Luciano Raúl
Ordoñez, Omar Federico
Martinez, Luciano José
Farias, Maria Eugenia
Albarracín, Virginia Helena
author_role author
author2 Portero, Luciano Raúl
Ordoñez, Omar Federico
Martinez, Luciano José
Farias, Maria Eugenia
Albarracín, Virginia Helena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioremediation
Acinetobacter
Exiguobacterium
Puna Region
topic Bioremediation
Acinetobacter
Exiguobacterium
Puna Region
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High levels of arsenic present in High Altitude Andean Lakes(HAAL) ecosystems have selected arsenic-resistant microbial communities which are of novel interest to study adaptations mechanisms which can be applied in bioremediation processes. We herein performed a detailedcharacterization of the arsenic tolerance profiles and the biofilm production oftwo HAAL poly-extremophiles, Acinetobacter sp. Ver3 (Ver3) and Exiguobacterium sp. S17 (S17). Cellular adherence over glass andpolypropylene surfaces were evaluated together with the effect of increasingdoses and oxidative states of arsenic over the quality and quantity of theirbiofilm production.The arsenic tolerance outcomes showed that HAAL strains couldtolerate higher arsenic concentrations than phylogenetically related strainsbelonging to the German collection of microorganisms and cell cultures(Deutsche Sammlung von Mikroorganismen und Zellkulturen, DSMZ), whichsuggest adaptations of HAAL strains to their original environment. On the otherhand, the crystal violet method (CV) and SEM analysis showed that Ver3 andS17 were able to attach to solid surfaces and to form the biofilm. Thequantification of biofilms production in 48 hours cultures through CV shows thatVer3 yielded higher production in the treatment without arsenic cultured on aglass support, while S17 yield higher biofilm production under intermediatearsenic concentration on glass supports. Polypropylene supports had negativeeffects on the biofilm production of Ver3 and S17. SEM analysis shows that the highest biofilm yields could be associated with a larger number of attached cells as well as the development of more complex 3D multicellular structures.
Fil: Zannier, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Portero, Luciano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martinez, Luciano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description High levels of arsenic present in High Altitude Andean Lakes(HAAL) ecosystems have selected arsenic-resistant microbial communities which are of novel interest to study adaptations mechanisms which can be applied in bioremediation processes. We herein performed a detailedcharacterization of the arsenic tolerance profiles and the biofilm production oftwo HAAL poly-extremophiles, Acinetobacter sp. Ver3 (Ver3) and Exiguobacterium sp. S17 (S17). Cellular adherence over glass andpolypropylene surfaces were evaluated together with the effect of increasingdoses and oxidative states of arsenic over the quality and quantity of theirbiofilm production.The arsenic tolerance outcomes showed that HAAL strains couldtolerate higher arsenic concentrations than phylogenetically related strainsbelonging to the German collection of microorganisms and cell cultures(Deutsche Sammlung von Mikroorganismen und Zellkulturen, DSMZ), whichsuggest adaptations of HAAL strains to their original environment. On the otherhand, the crystal violet method (CV) and SEM analysis showed that Ver3 andS17 were able to attach to solid surfaces and to form the biofilm. Thequantification of biofilms production in 48 hours cultures through CV shows thatVer3 yielded higher production in the treatment without arsenic cultured on aglass support, while S17 yield higher biofilm production under intermediatearsenic concentration on glass supports. Polypropylene supports had negativeeffects on the biofilm production of Ver3 and S17. SEM analysis shows that the highest biofilm yields could be associated with a larger number of attached cells as well as the development of more complex 3D multicellular structures.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82286
Zannier, Federico; Portero, Luciano Raúl; Ordoñez, Omar Federico; Martinez, Luciano José; Farias, Maria Eugenia; et al.; Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production; Hindawi Publishing Corporation; BioMed Research International; 2019; 1-2019; 1-12
2314-6133
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82286
identifier_str_mv Zannier, Federico; Portero, Luciano Raúl; Ordoñez, Omar Federico; Martinez, Luciano José; Farias, Maria Eugenia; et al.; Poly-extremophilic bacteria from high altitude Andean lakes: arsenic resistance profiles and biofilm production; Hindawi Publishing Corporation; BioMed Research International; 2019; 1-2019; 1-12
2314-6133
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2019/1231975/abs/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2019/1231975
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613243691073536
score 13.069144

Publicaciones similares