Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions

Autores
Stelletta, C.; Alberti, S.; Cil, B.; Tekin, K.; Tirpan, M. B.; Argañaraz, Martin Eduardo; Akcay, E.; Daskin, A.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The identification of various substances in seminal plasma has opened the way to study their functionality. It was aimed to identify the electrophoretic protein profile (EPP) and biochemical parameters (BP) of seminal plasma (SP) as predictors of semen quality and fertility in stallion. Forty-six ejaculates from 7 fertile stallions, aged between 6-26 years, were collected from May to July and 117 mares were used to obtain fertility data. For each ejaculate, volume, sperm motility, concentration were determined and seminal plasma samples were collected to perform one-dimensional electrophoresis and biochemical profiling. Following the estrus detection, mares were inseminated with fresh sperm. Pregnancy rates and foal rates were recorded. The concentration of 15-18 kDa molecular weight (MW) proteins has shown a positive correlation with sperm concentration and foal rate. Besides, a strong positive correlation was found between sperm concentration and 23-28 kDa MW proteins (r=0.77). The volume of 19-22 kDa MW proteins was negatively correlated with pregnancy and foal rate. Similarly, the volume of high MW proteins (173-385 kDa) correlated negatively with sperm motility and foal rate. Apart from the protein profile, while Magnesium and Glucose levels were negatively correlated with sperm quality and foal rate, Cholesterol level was a positive indicator of the quality of semen as well as the foaling rate. Moreover, the total protein level was correlated negatively with the sperm concentration whereas triglyceride was correlated positively. In conclusion, EPP and BP of seminal plasma are valuable clinical tools as predictors of fertility and semen quality in the stallion.
Fil: Stelletta, C.. Università di Padova; Italia
Fil: Alberti, S.. Università di Padova; Italia
Fil: Cil, B.. Ankara University; Turquía
Fil: Tekin, K.. Ankara University; Turquía
Fil: Tirpan, M. B.. Ankara University; Turquía
Fil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Akcay, E.. Ankara University; Turquía
Fil: Daskin, A.. Ankara University; Turquía
Materia
FERTILITY
SEMINAL PLASMA BIOCHEMISTRY
SEMINAL PLASMA PROTEINS
STALLION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210573
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/210573
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallionsStelletta, C.Alberti, S.Cil, B.Tekin, K.Tirpan, M. B.Argañaraz, Martin EduardoAkcay, E.Daskin, A.FERTILITYSEMINAL PLASMA BIOCHEMISTRYSEMINAL PLASMA PROTEINSSTALLIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The identification of various substances in seminal plasma has opened the way to study their functionality. It was aimed to identify the electrophoretic protein profile (EPP) and biochemical parameters (BP) of seminal plasma (SP) as predictors of semen quality and fertility in stallion. Forty-six ejaculates from 7 fertile stallions, aged between 6-26 years, were collected from May to July and 117 mares were used to obtain fertility data. For each ejaculate, volume, sperm motility, concentration were determined and seminal plasma samples were collected to perform one-dimensional electrophoresis and biochemical profiling. Following the estrus detection, mares were inseminated with fresh sperm. Pregnancy rates and foal rates were recorded. The concentration of 15-18 kDa molecular weight (MW) proteins has shown a positive correlation with sperm concentration and foal rate. Besides, a strong positive correlation was found between sperm concentration and 23-28 kDa MW proteins (r=0.77). The volume of 19-22 kDa MW proteins was negatively correlated with pregnancy and foal rate. Similarly, the volume of high MW proteins (173-385 kDa) correlated negatively with sperm motility and foal rate. Apart from the protein profile, while Magnesium and Glucose levels were negatively correlated with sperm quality and foal rate, Cholesterol level was a positive indicator of the quality of semen as well as the foaling rate. Moreover, the total protein level was correlated negatively with the sperm concentration whereas triglyceride was correlated positively. In conclusion, EPP and BP of seminal plasma are valuable clinical tools as predictors of fertility and semen quality in the stallion.Fil: Stelletta, C.. Università di Padova; ItaliaFil: Alberti, S.. Università di Padova; ItaliaFil: Cil, B.. Ankara University; TurquíaFil: Tekin, K.. Ankara University; TurquíaFil: Tirpan, M. B.. Ankara University; TurquíaFil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Akcay, E.. Ankara University; TurquíaFil: Daskin, A.. Ankara University; TurquíaPolish Academy of Sciences2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210573Stelletta, C.; Alberti, S.; Cil, B.; Tekin, K.; Tirpan, M. B.; et al.; Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions; Polish Academy of Sciences; Polish Journal Of Veterinary Sciences; 24; 4; 1-2022; 505-5141505-1773CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.24425/pjvs.2021.139975info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:44Zoai:ri.conicet.gov.ar:11336/210573instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:44.64CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
title Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
spellingShingle Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
Stelletta, C.
FERTILITY
SEMINAL PLASMA BIOCHEMISTRY
SEMINAL PLASMA PROTEINS
STALLION
title_short Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
title_full Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
title_fullStr Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
title_full_unstemmed Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
title_sort Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
dc.creator.none.fl_str_mv Stelletta, C.
Alberti, S.
Cil, B.
Tekin, K.
Tirpan, M. B.
Argañaraz, Martin Eduardo
Akcay, E.
Daskin, A.
author Stelletta, C.
author_facet Stelletta, C.
Alberti, S.
Cil, B.
Tekin, K.
Tirpan, M. B.
Argañaraz, Martin Eduardo
Akcay, E.
Daskin, A.
author_role author
author2 Alberti, S.
Cil, B.
Tekin, K.
Tirpan, M. B.
Argañaraz, Martin Eduardo
Akcay, E.
Daskin, A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FERTILITY
SEMINAL PLASMA BIOCHEMISTRY
SEMINAL PLASMA PROTEINS
STALLION
topic FERTILITY
SEMINAL PLASMA BIOCHEMISTRY
SEMINAL PLASMA PROTEINS
STALLION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The identification of various substances in seminal plasma has opened the way to study their functionality. It was aimed to identify the electrophoretic protein profile (EPP) and biochemical parameters (BP) of seminal plasma (SP) as predictors of semen quality and fertility in stallion. Forty-six ejaculates from 7 fertile stallions, aged between 6-26 years, were collected from May to July and 117 mares were used to obtain fertility data. For each ejaculate, volume, sperm motility, concentration were determined and seminal plasma samples were collected to perform one-dimensional electrophoresis and biochemical profiling. Following the estrus detection, mares were inseminated with fresh sperm. Pregnancy rates and foal rates were recorded. The concentration of 15-18 kDa molecular weight (MW) proteins has shown a positive correlation with sperm concentration and foal rate. Besides, a strong positive correlation was found between sperm concentration and 23-28 kDa MW proteins (r=0.77). The volume of 19-22 kDa MW proteins was negatively correlated with pregnancy and foal rate. Similarly, the volume of high MW proteins (173-385 kDa) correlated negatively with sperm motility and foal rate. Apart from the protein profile, while Magnesium and Glucose levels were negatively correlated with sperm quality and foal rate, Cholesterol level was a positive indicator of the quality of semen as well as the foaling rate. Moreover, the total protein level was correlated negatively with the sperm concentration whereas triglyceride was correlated positively. In conclusion, EPP and BP of seminal plasma are valuable clinical tools as predictors of fertility and semen quality in the stallion.
Fil: Stelletta, C.. Università di Padova; Italia
Fil: Alberti, S.. Università di Padova; Italia
Fil: Cil, B.. Ankara University; Turquía
Fil: Tekin, K.. Ankara University; Turquía
Fil: Tirpan, M. B.. Ankara University; Turquía
Fil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Akcay, E.. Ankara University; Turquía
Fil: Daskin, A.. Ankara University; Turquía
description The identification of various substances in seminal plasma has opened the way to study their functionality. It was aimed to identify the electrophoretic protein profile (EPP) and biochemical parameters (BP) of seminal plasma (SP) as predictors of semen quality and fertility in stallion. Forty-six ejaculates from 7 fertile stallions, aged between 6-26 years, were collected from May to July and 117 mares were used to obtain fertility data. For each ejaculate, volume, sperm motility, concentration were determined and seminal plasma samples were collected to perform one-dimensional electrophoresis and biochemical profiling. Following the estrus detection, mares were inseminated with fresh sperm. Pregnancy rates and foal rates were recorded. The concentration of 15-18 kDa molecular weight (MW) proteins has shown a positive correlation with sperm concentration and foal rate. Besides, a strong positive correlation was found between sperm concentration and 23-28 kDa MW proteins (r=0.77). The volume of 19-22 kDa MW proteins was negatively correlated with pregnancy and foal rate. Similarly, the volume of high MW proteins (173-385 kDa) correlated negatively with sperm motility and foal rate. Apart from the protein profile, while Magnesium and Glucose levels were negatively correlated with sperm quality and foal rate, Cholesterol level was a positive indicator of the quality of semen as well as the foaling rate. Moreover, the total protein level was correlated negatively with the sperm concentration whereas triglyceride was correlated positively. In conclusion, EPP and BP of seminal plasma are valuable clinical tools as predictors of fertility and semen quality in the stallion.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210573
Stelletta, C.; Alberti, S.; Cil, B.; Tekin, K.; Tirpan, M. B.; et al.; Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions; Polish Academy of Sciences; Polish Journal Of Veterinary Sciences; 24; 4; 1-2022; 505-514
1505-1773
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210573
identifier_str_mv Stelletta, C.; Alberti, S.; Cil, B.; Tekin, K.; Tirpan, M. B.; et al.; Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions; Polish Academy of Sciences; Polish Journal Of Veterinary Sciences; 24; 4; 1-2022; 505-514
1505-1773
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.24425/pjvs.2021.139975
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Polish Academy of Sciences
publisher.none.fl_str_mv Polish Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268684924485632
score 13.13397

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