Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina

Autores
Ledesma, Alba; Manes, J.; Rios, Glenda Laura; Aller, J.; Cesari, Andreina; Alberio, R.; Hozbor, F.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or artificial vagina on sperm resistance to cryodamage. Seminal plasma of five rams of the Corriedale breed collected by artificial vagina or electroejaculation was added before freezing to sperm cells collected by the two methods, and post-thaw quality parameters were evaluated. We found that seminal plasma has no effect on sperm resistance to cryodamage. However, we observed significantly higher percentages of sperm with intact and functional plasma membrane, intact acrosome and greater fertilizing potential after thawing in samples obtained by electroejaculation. This study demonstrates that sperm collected by electroejaculation are more resistant to damage caused by cryopreservation than those collected by artificial vagina.
Fil: Ledesma, Alba. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Manes, J.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
Fil: Rios, Glenda Laura. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aller, J.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Alberio, R.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
Fil: Hozbor, F.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
Materia
Seminal Plasma
Ram
Cryopreservation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13283
Acceder
id CONICETDig_d4aa40a9a47d0e8aaa5c2bf23be8fd18
oai_identifier_str oai:ri.conicet.gov.ar:11336/13283
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vaginaLedesma, AlbaManes, J.Rios, Glenda LauraAller, J.Cesari, AndreinaAlberio, R.Hozbor, F.Seminal PlasmaRamCryopreservationhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or artificial vagina on sperm resistance to cryodamage. Seminal plasma of five rams of the Corriedale breed collected by artificial vagina or electroejaculation was added before freezing to sperm cells collected by the two methods, and post-thaw quality parameters were evaluated. We found that seminal plasma has no effect on sperm resistance to cryodamage. However, we observed significantly higher percentages of sperm with intact and functional plasma membrane, intact acrosome and greater fertilizing potential after thawing in samples obtained by electroejaculation. This study demonstrates that sperm collected by electroejaculation are more resistant to damage caused by cryopreservation than those collected by artificial vagina.Fil: Ledesma, Alba. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Manes, J.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; ArgentinaFil: Rios, Glenda Laura. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aller, J.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; ArgentinaFil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Alberio, R.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; ArgentinaFil: Hozbor, F.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; ArgentinaWiley Blackwell Publishing, Inc2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13283Ledesma, Alba; Manes, J.; Rios, Glenda Laura; Aller, J.; Cesari, Andreina; et al.; Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 3; 1-2015; 386-3920936-67681439-0531enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12500/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12500info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:39Zoai:ri.conicet.gov.ar:11336/13283instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:40.27CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
title Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
spellingShingle Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
Ledesma, Alba
Seminal Plasma
Ram
Cryopreservation
title_short Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
title_full Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
title_fullStr Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
title_full_unstemmed Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
title_sort Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina
dc.creator.none.fl_str_mv Ledesma, Alba
Manes, J.
Rios, Glenda Laura
Aller, J.
Cesari, Andreina
Alberio, R.
Hozbor, F.
author Ledesma, Alba
author_facet Ledesma, Alba
Manes, J.
Rios, Glenda Laura
Aller, J.
Cesari, Andreina
Alberio, R.
Hozbor, F.
author_role author
author2 Manes, J.
Rios, Glenda Laura
Aller, J.
Cesari, Andreina
Alberio, R.
Hozbor, F.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Seminal Plasma
Ram
Cryopreservation
topic Seminal Plasma
Ram
Cryopreservation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or artificial vagina on sperm resistance to cryodamage. Seminal plasma of five rams of the Corriedale breed collected by artificial vagina or electroejaculation was added before freezing to sperm cells collected by the two methods, and post-thaw quality parameters were evaluated. We found that seminal plasma has no effect on sperm resistance to cryodamage. However, we observed significantly higher percentages of sperm with intact and functional plasma membrane, intact acrosome and greater fertilizing potential after thawing in samples obtained by electroejaculation. This study demonstrates that sperm collected by electroejaculation are more resistant to damage caused by cryopreservation than those collected by artificial vagina.
Fil: Ledesma, Alba. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Manes, J.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
Fil: Rios, Glenda Laura. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aller, J.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Alberio, R.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
Fil: Hozbor, F.. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires. Estacion Exptal.agrop.balcarce. Laboratorio de Biotecnologia de la Reproduccion; Argentina
description We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or artificial vagina on sperm resistance to cryodamage. Seminal plasma of five rams of the Corriedale breed collected by artificial vagina or electroejaculation was added before freezing to sperm cells collected by the two methods, and post-thaw quality parameters were evaluated. We found that seminal plasma has no effect on sperm resistance to cryodamage. However, we observed significantly higher percentages of sperm with intact and functional plasma membrane, intact acrosome and greater fertilizing potential after thawing in samples obtained by electroejaculation. This study demonstrates that sperm collected by electroejaculation are more resistant to damage caused by cryopreservation than those collected by artificial vagina.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13283
Ledesma, Alba; Manes, J.; Rios, Glenda Laura; Aller, J.; Cesari, Andreina; et al.; Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 3; 1-2015; 386-392
0936-6768
1439-0531
url http://hdl.handle.net/11336/13283
identifier_str_mv Ledesma, Alba; Manes, J.; Rios, Glenda Laura; Aller, J.; Cesari, Andreina; et al.; Effect of seminal plasma on post-thaw quality and functionality of corriedale ram sperm obtained by electroejaculation and artificial vagina; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 3; 1-2015; 386-392
0936-6768
1439-0531
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12500/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12500
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269652060733440
score 12.885934

Publicaciones similares