Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old
- Autores
- Rosales, Eliana; Bonilla, José Oscar; Cabral Bombardieri, Florencia; Peñalva, Julieta Aleandra; Mastrodonato, Anna Chiara; Esteves, Blanca Hebe; Molina, Luciana Vanina; Jofre, Claudio Francisco; Aguilera, Leonardo Javier; Campos, Ludmila Estefanía; Icazatti, Alejandro; Quijano, Federico; Pérez Diaz, Matias Federico Francisco; Carrizo, Erica Vanessa; Sanchez, Laura; Guevara, Alicia; Rodriguez, Daiana; Dufour, R.; Lacaze, Maria Agustina; Talia, Juan Manuel
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The Human Papilloma Virus (HPV) is the most important etiological agent related to cervical cancer (CC). This virus is mainly sexually-transmitted and, for this reason, it appears with high prevalence in sexually active women. The most frequent oncopathogenic HPV genotypes related to cancer are 16-HPV and 18-HPV. The low-risk strains 6-HPV and 11-HPV cause genital warts, but they are notassociated with cancer. The discovery of the causal correlation of CC with High Risk-HPV strains (HR-HPV), added to the long pre-neoplastic and pre-invasive period of the disease, gives us the opportunity to track down the disease through the Papanicolau Test (PAP) and molecular diagnosis. The objective of this work is to confirm the effectiveness of the molecular diagnosis for the earlydetection of HPV-oncopathogenic strains prior to the appearance of lesions, which are observable with PAP. Since June 2019, the PLSP began the molecular diagnosis of HPV in women older than 30 years by the Cobas® 4800 HPV Test System, approved by the FDA (Roche Molecular Systems Inc., Alameda, CA, USA), where HR-HPV DNA is detected and genotyped by real-time PCR. The PAP datawas obtained from SITAM (Ministerio de Salud de la Nación). This assay simultaneously detects a total of 14 HR-HPV types: 16-HPV individually, 18-HPV individually, and 12 pooled HR-HPV genotypes (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68). In our study, 3647 endocervical cytology specimens were collected from women over 30 years old. A total of 613 samples (16.80%) were positive forthe HPV test. Among these positive samples, 159 (25.94%) presented observable PAP-lesions, 339 (55.30%) did not present an observable lesion and 115 samples (18.76%) were only diagnosed for HPV using the molecular method, in the absence of PAP. In all the age-ranges evaluated (30-35 years, 36-40 years, 41-45 years, 46-50 years and >50 years) we observed a highly significant percentage of samples positive for the HPV test without observable lesion in the PAP, or only diagnosed for HPV using the molecular method. The obtained results allow us to affirm that the molecular detection of HR-HPV carried out in our laboratory is of great value for the prevention of CC in women older than 30 years. The early detection of HR-HPV strains allows us to start monitoring the patient immediately and prior to the appearance of lesions or CC, with great chances of overcoming the disease without major inconveniences.
Fil: Rosales, Eliana. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Bonilla, José Oscar. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Cabral Bombardieri, Florencia. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Peñalva, Julieta Aleandra. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Mastrodonato, Anna Chiara. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Esteves, Blanca Hebe. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Molina, Luciana Vanina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Jofre, Claudio Francisco. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Aguilera, Leonardo Javier. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Campos, Ludmila Estefanía. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Icazatti, Alejandro. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Quijano, Federico. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Pérez Diaz, Matias Federico Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Carrizo, Erica Vanessa. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Sanchez, Laura. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Guevara, Alicia. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Rodriguez, Daiana. Ministerio de Salud de San Luis; Argentina
Fil: Dufour, R.. Ministerio de Salud de San Luis; Argentina
Fil: Lacaze, Maria Agustina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
Fil: Talia, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina
XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo - Materia
-
MOLECULAR DIAGNOSIS
PAPILLOMA
STRATEGY
CANCER - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/281918
Ver los metadatos del registro completo
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Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years oldRosales, ElianaBonilla, José OscarCabral Bombardieri, FlorenciaPeñalva, Julieta AleandraMastrodonato, Anna ChiaraEsteves, Blanca HebeMolina, Luciana VaninaJofre, Claudio FranciscoAguilera, Leonardo JavierCampos, Ludmila EstefaníaIcazatti, AlejandroQuijano, FedericoPérez Diaz, Matias Federico FranciscoCarrizo, Erica VanessaSanchez, LauraGuevara, AliciaRodriguez, DaianaDufour, R.Lacaze, Maria AgustinaTalia, Juan ManuelMOLECULAR DIAGNOSISPAPILLOMASTRATEGYCANCERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Human Papilloma Virus (HPV) is the most important etiological agent related to cervical cancer (CC). This virus is mainly sexually-transmitted and, for this reason, it appears with high prevalence in sexually active women. The most frequent oncopathogenic HPV genotypes related to cancer are 16-HPV and 18-HPV. The low-risk strains 6-HPV and 11-HPV cause genital warts, but they are notassociated with cancer. The discovery of the causal correlation of CC with High Risk-HPV strains (HR-HPV), added to the long pre-neoplastic and pre-invasive period of the disease, gives us the opportunity to track down the disease through the Papanicolau Test (PAP) and molecular diagnosis. The objective of this work is to confirm the effectiveness of the molecular diagnosis for the earlydetection of HPV-oncopathogenic strains prior to the appearance of lesions, which are observable with PAP. Since June 2019, the PLSP began the molecular diagnosis of HPV in women older than 30 years by the Cobas® 4800 HPV Test System, approved by the FDA (Roche Molecular Systems Inc., Alameda, CA, USA), where HR-HPV DNA is detected and genotyped by real-time PCR. The PAP datawas obtained from SITAM (Ministerio de Salud de la Nación). This assay simultaneously detects a total of 14 HR-HPV types: 16-HPV individually, 18-HPV individually, and 12 pooled HR-HPV genotypes (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68). In our study, 3647 endocervical cytology specimens were collected from women over 30 years old. A total of 613 samples (16.80%) were positive forthe HPV test. Among these positive samples, 159 (25.94%) presented observable PAP-lesions, 339 (55.30%) did not present an observable lesion and 115 samples (18.76%) were only diagnosed for HPV using the molecular method, in the absence of PAP. In all the age-ranges evaluated (30-35 years, 36-40 years, 41-45 years, 46-50 years and >50 years) we observed a highly significant percentage of samples positive for the HPV test without observable lesion in the PAP, or only diagnosed for HPV using the molecular method. The obtained results allow us to affirm that the molecular detection of HR-HPV carried out in our laboratory is of great value for the prevention of CC in women older than 30 years. The early detection of HR-HPV strains allows us to start monitoring the patient immediately and prior to the appearance of lesions or CC, with great chances of overcoming the disease without major inconveniences.Fil: Rosales, Eliana. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Bonilla, José Oscar. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Cabral Bombardieri, Florencia. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Peñalva, Julieta Aleandra. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Mastrodonato, Anna Chiara. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Esteves, Blanca Hebe. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Molina, Luciana Vanina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Jofre, Claudio Francisco. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Aguilera, Leonardo Javier. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Campos, Ludmila Estefanía. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Icazatti, Alejandro. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Quijano, Federico. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Pérez Diaz, Matias Federico Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Carrizo, Erica Vanessa. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Sanchez, Laura. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Guevara, Alicia. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Rodriguez, Daiana. Ministerio de Salud de San Luis; ArgentinaFil: Dufour, R.. Ministerio de Salud de San Luis; ArgentinaFil: Lacaze, Maria Agustina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaFil: Talia, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; ArgentinaXXXIX Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281918Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2021; 1-40327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www-techscience-com.translate.goog/journal/biocell?_x_tr_sl=en&_x_tr_tl=es&_x_tr_hl=es&_x_tr_pto=tcNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T15:24:49Zoai:ri.conicet.gov.ar:11336/281918instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 15:24:49.784CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old |
| title |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old |
| spellingShingle |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old Rosales, Eliana MOLECULAR DIAGNOSIS PAPILLOMA STRATEGY CANCER |
| title_short |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old |
| title_full |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old |
| title_fullStr |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old |
| title_full_unstemmed |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old |
| title_sort |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old |
| dc.creator.none.fl_str_mv |
Rosales, Eliana Bonilla, José Oscar Cabral Bombardieri, Florencia Peñalva, Julieta Aleandra Mastrodonato, Anna Chiara Esteves, Blanca Hebe Molina, Luciana Vanina Jofre, Claudio Francisco Aguilera, Leonardo Javier Campos, Ludmila Estefanía Icazatti, Alejandro Quijano, Federico Pérez Diaz, Matias Federico Francisco Carrizo, Erica Vanessa Sanchez, Laura Guevara, Alicia Rodriguez, Daiana Dufour, R. Lacaze, Maria Agustina Talia, Juan Manuel |
| author |
Rosales, Eliana |
| author_facet |
Rosales, Eliana Bonilla, José Oscar Cabral Bombardieri, Florencia Peñalva, Julieta Aleandra Mastrodonato, Anna Chiara Esteves, Blanca Hebe Molina, Luciana Vanina Jofre, Claudio Francisco Aguilera, Leonardo Javier Campos, Ludmila Estefanía Icazatti, Alejandro Quijano, Federico Pérez Diaz, Matias Federico Francisco Carrizo, Erica Vanessa Sanchez, Laura Guevara, Alicia Rodriguez, Daiana Dufour, R. Lacaze, Maria Agustina Talia, Juan Manuel |
| author_role |
author |
| author2 |
Bonilla, José Oscar Cabral Bombardieri, Florencia Peñalva, Julieta Aleandra Mastrodonato, Anna Chiara Esteves, Blanca Hebe Molina, Luciana Vanina Jofre, Claudio Francisco Aguilera, Leonardo Javier Campos, Ludmila Estefanía Icazatti, Alejandro Quijano, Federico Pérez Diaz, Matias Federico Francisco Carrizo, Erica Vanessa Sanchez, Laura Guevara, Alicia Rodriguez, Daiana Dufour, R. Lacaze, Maria Agustina Talia, Juan Manuel |
| author2_role |
author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
MOLECULAR DIAGNOSIS PAPILLOMA STRATEGY CANCER |
| topic |
MOLECULAR DIAGNOSIS PAPILLOMA STRATEGY CANCER |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The Human Papilloma Virus (HPV) is the most important etiological agent related to cervical cancer (CC). This virus is mainly sexually-transmitted and, for this reason, it appears with high prevalence in sexually active women. The most frequent oncopathogenic HPV genotypes related to cancer are 16-HPV and 18-HPV. The low-risk strains 6-HPV and 11-HPV cause genital warts, but they are notassociated with cancer. The discovery of the causal correlation of CC with High Risk-HPV strains (HR-HPV), added to the long pre-neoplastic and pre-invasive period of the disease, gives us the opportunity to track down the disease through the Papanicolau Test (PAP) and molecular diagnosis. The objective of this work is to confirm the effectiveness of the molecular diagnosis for the earlydetection of HPV-oncopathogenic strains prior to the appearance of lesions, which are observable with PAP. Since June 2019, the PLSP began the molecular diagnosis of HPV in women older than 30 years by the Cobas® 4800 HPV Test System, approved by the FDA (Roche Molecular Systems Inc., Alameda, CA, USA), where HR-HPV DNA is detected and genotyped by real-time PCR. The PAP datawas obtained from SITAM (Ministerio de Salud de la Nación). This assay simultaneously detects a total of 14 HR-HPV types: 16-HPV individually, 18-HPV individually, and 12 pooled HR-HPV genotypes (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68). In our study, 3647 endocervical cytology specimens were collected from women over 30 years old. A total of 613 samples (16.80%) were positive forthe HPV test. Among these positive samples, 159 (25.94%) presented observable PAP-lesions, 339 (55.30%) did not present an observable lesion and 115 samples (18.76%) were only diagnosed for HPV using the molecular method, in the absence of PAP. In all the age-ranges evaluated (30-35 years, 36-40 years, 41-45 years, 46-50 years and >50 years) we observed a highly significant percentage of samples positive for the HPV test without observable lesion in the PAP, or only diagnosed for HPV using the molecular method. The obtained results allow us to affirm that the molecular detection of HR-HPV carried out in our laboratory is of great value for the prevention of CC in women older than 30 years. The early detection of HR-HPV strains allows us to start monitoring the patient immediately and prior to the appearance of lesions or CC, with great chances of overcoming the disease without major inconveniences. Fil: Rosales, Eliana. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Bonilla, José Oscar. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Cabral Bombardieri, Florencia. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Peñalva, Julieta Aleandra. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Mastrodonato, Anna Chiara. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina Fil: Esteves, Blanca Hebe. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Molina, Luciana Vanina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Jofre, Claudio Francisco. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Aguilera, Leonardo Javier. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Campos, Ludmila Estefanía. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Icazatti, Alejandro. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Quijano, Federico. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Pérez Diaz, Matias Federico Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Carrizo, Erica Vanessa. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Sanchez, Laura. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Guevara, Alicia. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Rodriguez, Daiana. Ministerio de Salud de San Luis; Argentina Fil: Dufour, R.. Ministerio de Salud de San Luis; Argentina Fil: Lacaze, Maria Agustina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina Fil: Talia, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Laboratorio de Salud Pública "dalmiro Perez Laborda"; Argentina XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo San Luis Argentina Sociedad de Biología de Cuyo |
| description |
The Human Papilloma Virus (HPV) is the most important etiological agent related to cervical cancer (CC). This virus is mainly sexually-transmitted and, for this reason, it appears with high prevalence in sexually active women. The most frequent oncopathogenic HPV genotypes related to cancer are 16-HPV and 18-HPV. The low-risk strains 6-HPV and 11-HPV cause genital warts, but they are notassociated with cancer. The discovery of the causal correlation of CC with High Risk-HPV strains (HR-HPV), added to the long pre-neoplastic and pre-invasive period of the disease, gives us the opportunity to track down the disease through the Papanicolau Test (PAP) and molecular diagnosis. The objective of this work is to confirm the effectiveness of the molecular diagnosis for the earlydetection of HPV-oncopathogenic strains prior to the appearance of lesions, which are observable with PAP. Since June 2019, the PLSP began the molecular diagnosis of HPV in women older than 30 years by the Cobas® 4800 HPV Test System, approved by the FDA (Roche Molecular Systems Inc., Alameda, CA, USA), where HR-HPV DNA is detected and genotyped by real-time PCR. The PAP datawas obtained from SITAM (Ministerio de Salud de la Nación). This assay simultaneously detects a total of 14 HR-HPV types: 16-HPV individually, 18-HPV individually, and 12 pooled HR-HPV genotypes (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68). In our study, 3647 endocervical cytology specimens were collected from women over 30 years old. A total of 613 samples (16.80%) were positive forthe HPV test. Among these positive samples, 159 (25.94%) presented observable PAP-lesions, 339 (55.30%) did not present an observable lesion and 115 samples (18.76%) were only diagnosed for HPV using the molecular method, in the absence of PAP. In all the age-ranges evaluated (30-35 years, 36-40 years, 41-45 years, 46-50 years and >50 years) we observed a highly significant percentage of samples positive for the HPV test without observable lesion in the PAP, or only diagnosed for HPV using the molecular method. The obtained results allow us to affirm that the molecular detection of HR-HPV carried out in our laboratory is of great value for the prevention of CC in women older than 30 years. The early detection of HR-HPV strains allows us to start monitoring the patient immediately and prior to the appearance of lesions or CC, with great chances of overcoming the disease without major inconveniences. |
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2021 |
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2021 |
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conferenceObject |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/281918 Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2021; 1-4 0327-9545 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/281918 |
| identifier_str_mv |
Molecular diagnosis of High Risk-Human Papilloma Virus (HR-HPV) as strategy for the prevention and detection of cervical cancer at the early stages in women over 30 years old; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2021; 1-4 0327-9545 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www-techscience-com.translate.goog/journal/biocell?_x_tr_sl=en&_x_tr_tl=es&_x_tr_hl=es&_x_tr_pto=tc |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
| dc.coverage.none.fl_str_mv |
Nacional |
| dc.publisher.none.fl_str_mv |
Sociedad de Biología de Cuyo |
| publisher.none.fl_str_mv |
Sociedad de Biología de Cuyo |
| dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
| reponame_str |
CONICET Digital (CONICET) |
| collection |
CONICET Digital (CONICET) |
| instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1861214732559581184 |
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12.822162 |