First report of linear megaplasmids in the genus Micrococcus

Autores
Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russel T.; Farias, Maria Eugenia; Meinhardt, Friedhelm
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High-altitude wetlands (above 4200 m) in the northwest of Argentina are considered pristine and extreme environments. Micrococcus sp. A1, H5, and V7, isolated from such environments, were shown to contain linear megaplasmids, designated pLMA1, pLMH5, and pLMV7, respectively. As known from linear plasmids of other actinomycetes, all three plasmids were resistant to λ exonuclease treatment, which is consistent with having terminal proteins covalently attached to their 5′ DNA ends. Electrophoretic mobility, Southern analysis, and restriction endonuclease patterns revealed pLMA1 and pLMH5 being indistinguishable plasmids, even though they were found in different strains isolated from two distant wetlands – Laguna Azul and Laguna Huaca Huasi. Analysis of 16S rDNA sequences of Micrococcus sp. A1, H5, and V7 suggested a close relationship to Micrococcus luteus. Typing of isolates was performed using fingerprint patterns generated by BOX-PCR. Plasmid-deficient strains, generated from Micrococcus sp. A1, showed a significantly decreased resistance level for erythromycin.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Wagenknecht, Martin. Westfalische Wilhelms Universitat; Alemania
Fil: Hill, Russel T.. University Of Maryland. Biotechnology Institute. Center Of Marine Biotechnology; Estados Unidos
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Meinhardt, Friedhelm. Westfalische Wilhelms Universitat; Alemania
Materia
Micrococcus
Linear Plasmid
Terminal Protein
Box-Pcr
Argentinean High-Altitude Wetlands
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41353
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of linear megaplasmids in the genus MicrococcusDib, Julian RafaelWagenknecht, MartinHill, Russel T.Farias, Maria EugeniaMeinhardt, FriedhelmMicrococcusLinear PlasmidTerminal ProteinBox-PcrArgentinean High-Altitude Wetlandshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High-altitude wetlands (above 4200 m) in the northwest of Argentina are considered pristine and extreme environments. Micrococcus sp. A1, H5, and V7, isolated from such environments, were shown to contain linear megaplasmids, designated pLMA1, pLMH5, and pLMV7, respectively. As known from linear plasmids of other actinomycetes, all three plasmids were resistant to λ exonuclease treatment, which is consistent with having terminal proteins covalently attached to their 5′ DNA ends. Electrophoretic mobility, Southern analysis, and restriction endonuclease patterns revealed pLMA1 and pLMH5 being indistinguishable plasmids, even though they were found in different strains isolated from two distant wetlands – Laguna Azul and Laguna Huaca Huasi. Analysis of 16S rDNA sequences of Micrococcus sp. A1, H5, and V7 suggested a close relationship to Micrococcus luteus. Typing of isolates was performed using fingerprint patterns generated by BOX-PCR. Plasmid-deficient strains, generated from Micrococcus sp. A1, showed a significantly decreased resistance level for erythromycin.Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Wagenknecht, Martin. Westfalische Wilhelms Universitat; AlemaniaFil: Hill, Russel T.. University Of Maryland. Biotechnology Institute. Center Of Marine Biotechnology; Estados UnidosFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Meinhardt, Friedhelm. Westfalische Wilhelms Universitat; AlemaniaAcademic Press Inc Elsevier Science2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41353Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russel T.; Farias, Maria Eugenia; Meinhardt, Friedhelm; First report of linear megaplasmids in the genus Micrococcus; Academic Press Inc Elsevier Science; Plasmid.; 63; 1; 12-2010; 40-450147-619XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147619X09001176info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plasmid.2009.10.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:16Zoai:ri.conicet.gov.ar:11336/41353instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:16.811CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of linear megaplasmids in the genus Micrococcus
title First report of linear megaplasmids in the genus Micrococcus
spellingShingle First report of linear megaplasmids in the genus Micrococcus
Dib, Julian Rafael
Micrococcus
Linear Plasmid
Terminal Protein
Box-Pcr
Argentinean High-Altitude Wetlands
title_short First report of linear megaplasmids in the genus Micrococcus
title_full First report of linear megaplasmids in the genus Micrococcus
title_fullStr First report of linear megaplasmids in the genus Micrococcus
title_full_unstemmed First report of linear megaplasmids in the genus Micrococcus
title_sort First report of linear megaplasmids in the genus Micrococcus
dc.creator.none.fl_str_mv Dib, Julian Rafael
Wagenknecht, Martin
Hill, Russel T.
Farias, Maria Eugenia
Meinhardt, Friedhelm
author Dib, Julian Rafael
author_facet Dib, Julian Rafael
Wagenknecht, Martin
Hill, Russel T.
Farias, Maria Eugenia
Meinhardt, Friedhelm
author_role author
author2 Wagenknecht, Martin
Hill, Russel T.
Farias, Maria Eugenia
Meinhardt, Friedhelm
author2_role author
author
author
author
dc.subject.none.fl_str_mv Micrococcus
Linear Plasmid
Terminal Protein
Box-Pcr
Argentinean High-Altitude Wetlands
topic Micrococcus
Linear Plasmid
Terminal Protein
Box-Pcr
Argentinean High-Altitude Wetlands
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High-altitude wetlands (above 4200 m) in the northwest of Argentina are considered pristine and extreme environments. Micrococcus sp. A1, H5, and V7, isolated from such environments, were shown to contain linear megaplasmids, designated pLMA1, pLMH5, and pLMV7, respectively. As known from linear plasmids of other actinomycetes, all three plasmids were resistant to λ exonuclease treatment, which is consistent with having terminal proteins covalently attached to their 5′ DNA ends. Electrophoretic mobility, Southern analysis, and restriction endonuclease patterns revealed pLMA1 and pLMH5 being indistinguishable plasmids, even though they were found in different strains isolated from two distant wetlands – Laguna Azul and Laguna Huaca Huasi. Analysis of 16S rDNA sequences of Micrococcus sp. A1, H5, and V7 suggested a close relationship to Micrococcus luteus. Typing of isolates was performed using fingerprint patterns generated by BOX-PCR. Plasmid-deficient strains, generated from Micrococcus sp. A1, showed a significantly decreased resistance level for erythromycin.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Wagenknecht, Martin. Westfalische Wilhelms Universitat; Alemania
Fil: Hill, Russel T.. University Of Maryland. Biotechnology Institute. Center Of Marine Biotechnology; Estados Unidos
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Meinhardt, Friedhelm. Westfalische Wilhelms Universitat; Alemania
description High-altitude wetlands (above 4200 m) in the northwest of Argentina are considered pristine and extreme environments. Micrococcus sp. A1, H5, and V7, isolated from such environments, were shown to contain linear megaplasmids, designated pLMA1, pLMH5, and pLMV7, respectively. As known from linear plasmids of other actinomycetes, all three plasmids were resistant to λ exonuclease treatment, which is consistent with having terminal proteins covalently attached to their 5′ DNA ends. Electrophoretic mobility, Southern analysis, and restriction endonuclease patterns revealed pLMA1 and pLMH5 being indistinguishable plasmids, even though they were found in different strains isolated from two distant wetlands – Laguna Azul and Laguna Huaca Huasi. Analysis of 16S rDNA sequences of Micrococcus sp. A1, H5, and V7 suggested a close relationship to Micrococcus luteus. Typing of isolates was performed using fingerprint patterns generated by BOX-PCR. Plasmid-deficient strains, generated from Micrococcus sp. A1, showed a significantly decreased resistance level for erythromycin.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41353
Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russel T.; Farias, Maria Eugenia; Meinhardt, Friedhelm; First report of linear megaplasmids in the genus Micrococcus; Academic Press Inc Elsevier Science; Plasmid.; 63; 1; 12-2010; 40-45
0147-619X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41353
identifier_str_mv Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russel T.; Farias, Maria Eugenia; Meinhardt, Friedhelm; First report of linear megaplasmids in the genus Micrococcus; Academic Press Inc Elsevier Science; Plasmid.; 63; 1; 12-2010; 40-45
0147-619X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147619X09001176
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plasmid.2009.10.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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