Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain

Autores
Dib, Julian Rafael; Farias, Maria Eugenia; Angelov, Angel; Liebl, Wolfgang
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Micrococci are Gram-positive, G+C-rich, nonmotile, non-spore-forming actinomycetous bacteria. Micrococcus strains have proven to play important roles in the biodegradation of xenobiotics, bioremediation processes, production of biotechnologically important enzymes or bioactive compounds, as test strains in biological assays for lysozyme and antibiotics. They can also cause infections in immunocompromised humans. We recently reported the isolation and characterization of the first linear plasmids in different strains of Micrococcus from extreme environments in Argentina. Among them, linear plasmid pLMA1 (110 kb) proved to be linked to an erythromycin resistance phenotype. In this report we show that this plasmid can be transferred to the type strain of M. luteus (NCTC 2665) in a process most closely resembling conjugation. However, clear differences from the classical conjugative plasmid transfer were observed, indicating the existence of a specialized system. We were further able to perform genetic manipulations on the transferred plasmid, which will allow the functional investigation of the elements involved in the transfer process. In addition, the transfer ability of the linear plasmid pLMA1, together with the rather efficient method for its manipulation, can establish it as an useful tool for biotechnological purposes in the genus Micrococcus The authors would like to acknowledge the support from the Bayerisches Hochschulzentrum für Lateinamerika (BAYLAT).
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Angelov, Angel. Technische Universitat München; Alemania
Fil: Liebl, Wolfgang. Technische Universitat München; Alemania
Annual Conference 2015 of the Association for General and Applied Microbiology
Marburg
Alemania
Association for General and Applied Microbiology
Materia
Micrococcus
Genetic engineering
Linear plasmid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195116
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195116
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strainDib, Julian RafaelFarias, Maria EugeniaAngelov, AngelLiebl, WolfgangMicrococcusGenetic engineeringLinear plasmidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Micrococci are Gram-positive, G+C-rich, nonmotile, non-spore-forming actinomycetous bacteria. Micrococcus strains have proven to play important roles in the biodegradation of xenobiotics, bioremediation processes, production of biotechnologically important enzymes or bioactive compounds, as test strains in biological assays for lysozyme and antibiotics. They can also cause infections in immunocompromised humans. We recently reported the isolation and characterization of the first linear plasmids in different strains of Micrococcus from extreme environments in Argentina. Among them, linear plasmid pLMA1 (110 kb) proved to be linked to an erythromycin resistance phenotype. In this report we show that this plasmid can be transferred to the type strain of M. luteus (NCTC 2665) in a process most closely resembling conjugation. However, clear differences from the classical conjugative plasmid transfer were observed, indicating the existence of a specialized system. We were further able to perform genetic manipulations on the transferred plasmid, which will allow the functional investigation of the elements involved in the transfer process. In addition, the transfer ability of the linear plasmid pLMA1, together with the rather efficient method for its manipulation, can establish it as an useful tool for biotechnological purposes in the genus Micrococcus The authors would like to acknowledge the support from the Bayerisches Hochschulzentrum für Lateinamerika (BAYLAT).Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Angelov, Angel. Technische Universitat München; AlemaniaFil: Liebl, Wolfgang. Technische Universitat München; AlemaniaAnnual Conference 2015 of the Association for General and Applied MicrobiologyMarburgAlemaniaAssociation for General and Applied MicrobiologySpringer2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195116Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain; Annual Conference 2015 of the Association for General and Applied Microbiology; Marburg; Alemania; 2015; 199-1990947-0867CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://vaam.de/media/vaam_tagungsband_2015.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:42Zoai:ri.conicet.gov.ar:11336/195116instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:42.345CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
title Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
spellingShingle Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
Dib, Julian Rafael
Micrococcus
Genetic engineering
Linear plasmid
title_short Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
title_full Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
title_fullStr Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
title_full_unstemmed Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
title_sort Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain
dc.creator.none.fl_str_mv Dib, Julian Rafael
Farias, Maria Eugenia
Angelov, Angel
Liebl, Wolfgang
author Dib, Julian Rafael
author_facet Dib, Julian Rafael
Farias, Maria Eugenia
Angelov, Angel
Liebl, Wolfgang
author_role author
author2 Farias, Maria Eugenia
Angelov, Angel
Liebl, Wolfgang
author2_role author
author
author
dc.subject.none.fl_str_mv Micrococcus
Genetic engineering
Linear plasmid
topic Micrococcus
Genetic engineering
Linear plasmid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Micrococci are Gram-positive, G+C-rich, nonmotile, non-spore-forming actinomycetous bacteria. Micrococcus strains have proven to play important roles in the biodegradation of xenobiotics, bioremediation processes, production of biotechnologically important enzymes or bioactive compounds, as test strains in biological assays for lysozyme and antibiotics. They can also cause infections in immunocompromised humans. We recently reported the isolation and characterization of the first linear plasmids in different strains of Micrococcus from extreme environments in Argentina. Among them, linear plasmid pLMA1 (110 kb) proved to be linked to an erythromycin resistance phenotype. In this report we show that this plasmid can be transferred to the type strain of M. luteus (NCTC 2665) in a process most closely resembling conjugation. However, clear differences from the classical conjugative plasmid transfer were observed, indicating the existence of a specialized system. We were further able to perform genetic manipulations on the transferred plasmid, which will allow the functional investigation of the elements involved in the transfer process. In addition, the transfer ability of the linear plasmid pLMA1, together with the rather efficient method for its manipulation, can establish it as an useful tool for biotechnological purposes in the genus Micrococcus The authors would like to acknowledge the support from the Bayerisches Hochschulzentrum für Lateinamerika (BAYLAT).
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Angelov, Angel. Technische Universitat München; Alemania
Fil: Liebl, Wolfgang. Technische Universitat München; Alemania
Annual Conference 2015 of the Association for General and Applied Microbiology
Marburg
Alemania
Association for General and Applied Microbiology
description Micrococci are Gram-positive, G+C-rich, nonmotile, non-spore-forming actinomycetous bacteria. Micrococcus strains have proven to play important roles in the biodegradation of xenobiotics, bioremediation processes, production of biotechnologically important enzymes or bioactive compounds, as test strains in biological assays for lysozyme and antibiotics. They can also cause infections in immunocompromised humans. We recently reported the isolation and characterization of the first linear plasmids in different strains of Micrococcus from extreme environments in Argentina. Among them, linear plasmid pLMA1 (110 kb) proved to be linked to an erythromycin resistance phenotype. In this report we show that this plasmid can be transferred to the type strain of M. luteus (NCTC 2665) in a process most closely resembling conjugation. However, clear differences from the classical conjugative plasmid transfer were observed, indicating the existence of a specialized system. We were further able to perform genetic manipulations on the transferred plasmid, which will allow the functional investigation of the elements involved in the transfer process. In addition, the transfer ability of the linear plasmid pLMA1, together with the rather efficient method for its manipulation, can establish it as an useful tool for biotechnological purposes in the genus Micrococcus The authors would like to acknowledge the support from the Bayerisches Hochschulzentrum für Lateinamerika (BAYLAT).
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195116
Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain; Annual Conference 2015 of the Association for General and Applied Microbiology; Marburg; Alemania; 2015; 199-199
0947-0867
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195116
identifier_str_mv Horizontal gene transfer and genetic engineering of linear plasmid pLMA1 in Micrococcus luteus Fleming strain; Annual Conference 2015 of the Association for General and Applied Microbiology; Marburg; Alemania; 2015; 199-199
0947-0867
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://vaam.de/media/vaam_tagungsband_2015.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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