Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae

Autores
Giménez Martínez, Pablo Darian; Zuñiga, Fabian; Junges, Celina Maria; Maggi, Matías Daniel; Fuselli, Sandra Rosa; Martínez, Jessica
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
American foulbrood caused by Paenibacillus larvae brings with it several problems on beekeepers for the use of synthetic antibiotics, likewise led to the appearance of resistant strains and the presence of chemical residues in the commercial products of the hive. In recent years, the use of natural compounds for the control of P. larvae has increased. Herein, we propose to determine the value of the Minimal Inhibitory Concentration (MIC) of a hydroalcoholic extract of Humulus lupulus (hop) leaves of Victoria variety on P. larvae strain, and to evaluate its toxicity and effect on the expression of three genes of the immune system on honey bee larvae. For the extract, the MIC value was 83.79 μg/mL. For larvae, the extract did not show toxicity, on the other hand presented a down-regulation in the gene expression for the three genes evaluated. The results obtained here are a first report on the evaluation in the effect of natural extracts on the expression of genes related to the immune system in bee larvae.
Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Zuñiga, Fabian. Universidad del Desarrollo; Chile
Fil: Junges, Celina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Martínez, Jessica. Universidad del Desarrollo; Chile
Materia
APIS MELLIFERA, PAENIBACILLUS LARVAE, ANTIMICROBIAL PEPTIDES
HOPS
SECONDARY METABOLITES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153332
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvaeGiménez Martínez, Pablo DarianZuñiga, FabianJunges, Celina MariaMaggi, Matías DanielFuselli, Sandra RosaMartínez, JessicaAPIS MELLIFERA, PAENIBACILLUS LARVAE, ANTIMICROBIAL PEPTIDESHOPSSECONDARY METABOLITEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4American foulbrood caused by Paenibacillus larvae brings with it several problems on beekeepers for the use of synthetic antibiotics, likewise led to the appearance of resistant strains and the presence of chemical residues in the commercial products of the hive. In recent years, the use of natural compounds for the control of P. larvae has increased. Herein, we propose to determine the value of the Minimal Inhibitory Concentration (MIC) of a hydroalcoholic extract of Humulus lupulus (hop) leaves of Victoria variety on P. larvae strain, and to evaluate its toxicity and effect on the expression of three genes of the immune system on honey bee larvae. For the extract, the MIC value was 83.79 μg/mL. For larvae, the extract did not show toxicity, on the other hand presented a down-regulation in the gene expression for the three genes evaluated. The results obtained here are a first report on the evaluation in the effect of natural extracts on the expression of genes related to the immune system in bee larvae.Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Zuñiga, Fabian. Universidad del Desarrollo; ChileFil: Junges, Celina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Martínez, Jessica. Universidad del Desarrollo; ChileEDP Sciences2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153332Giménez Martínez, Pablo Darian; Zuñiga, Fabian; Junges, Celina Maria; Maggi, Matías Daniel; Fuselli, Sandra Rosa; et al.; Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae; EDP Sciences; Apidologie; 52; 4; 5-2021; 813-8240044-8435CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s13592-021-00868-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s13592-021-00868-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:16Zoai:ri.conicet.gov.ar:11336/153332instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:17.033CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
title Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
spellingShingle Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
Giménez Martínez, Pablo Darian
APIS MELLIFERA, PAENIBACILLUS LARVAE, ANTIMICROBIAL PEPTIDES
HOPS
SECONDARY METABOLITES
title_short Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
title_full Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
title_fullStr Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
title_full_unstemmed Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
title_sort Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae
dc.creator.none.fl_str_mv Giménez Martínez, Pablo Darian
Zuñiga, Fabian
Junges, Celina Maria
Maggi, Matías Daniel
Fuselli, Sandra Rosa
Martínez, Jessica
author Giménez Martínez, Pablo Darian
author_facet Giménez Martínez, Pablo Darian
Zuñiga, Fabian
Junges, Celina Maria
Maggi, Matías Daniel
Fuselli, Sandra Rosa
Martínez, Jessica
author_role author
author2 Zuñiga, Fabian
Junges, Celina Maria
Maggi, Matías Daniel
Fuselli, Sandra Rosa
Martínez, Jessica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv APIS MELLIFERA, PAENIBACILLUS LARVAE, ANTIMICROBIAL PEPTIDES
HOPS
SECONDARY METABOLITES
topic APIS MELLIFERA, PAENIBACILLUS LARVAE, ANTIMICROBIAL PEPTIDES
HOPS
SECONDARY METABOLITES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv American foulbrood caused by Paenibacillus larvae brings with it several problems on beekeepers for the use of synthetic antibiotics, likewise led to the appearance of resistant strains and the presence of chemical residues in the commercial products of the hive. In recent years, the use of natural compounds for the control of P. larvae has increased. Herein, we propose to determine the value of the Minimal Inhibitory Concentration (MIC) of a hydroalcoholic extract of Humulus lupulus (hop) leaves of Victoria variety on P. larvae strain, and to evaluate its toxicity and effect on the expression of three genes of the immune system on honey bee larvae. For the extract, the MIC value was 83.79 μg/mL. For larvae, the extract did not show toxicity, on the other hand presented a down-regulation in the gene expression for the three genes evaluated. The results obtained here are a first report on the evaluation in the effect of natural extracts on the expression of genes related to the immune system in bee larvae.
Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Zuñiga, Fabian. Universidad del Desarrollo; Chile
Fil: Junges, Celina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Martínez, Jessica. Universidad del Desarrollo; Chile
description American foulbrood caused by Paenibacillus larvae brings with it several problems on beekeepers for the use of synthetic antibiotics, likewise led to the appearance of resistant strains and the presence of chemical residues in the commercial products of the hive. In recent years, the use of natural compounds for the control of P. larvae has increased. Herein, we propose to determine the value of the Minimal Inhibitory Concentration (MIC) of a hydroalcoholic extract of Humulus lupulus (hop) leaves of Victoria variety on P. larvae strain, and to evaluate its toxicity and effect on the expression of three genes of the immune system on honey bee larvae. For the extract, the MIC value was 83.79 μg/mL. For larvae, the extract did not show toxicity, on the other hand presented a down-regulation in the gene expression for the three genes evaluated. The results obtained here are a first report on the evaluation in the effect of natural extracts on the expression of genes related to the immune system in bee larvae.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153332
Giménez Martínez, Pablo Darian; Zuñiga, Fabian; Junges, Celina Maria; Maggi, Matías Daniel; Fuselli, Sandra Rosa; et al.; Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae; EDP Sciences; Apidologie; 52; 4; 5-2021; 813-824
0044-8435
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153332
identifier_str_mv Giménez Martínez, Pablo Darian; Zuñiga, Fabian; Junges, Celina Maria; Maggi, Matías Daniel; Fuselli, Sandra Rosa; et al.; Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae; EDP Sciences; Apidologie; 52; 4; 5-2021; 813-824
0044-8435
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s13592-021-00868-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13592-021-00868-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv EDP Sciences
publisher.none.fl_str_mv EDP Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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