Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)

Autores
Giménez Martínez, Pablo Darian; Cugnata, Noelia Melina; Alonso Salces, Rosa Maria; Arredondo, Daniela; Antúnez, Karina; de Castro, Rosana Esther; Fuselli, Sandra Rosa
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim of study: To evaluate the potential bactericidal activity of natural molecules against Paenibacillus larvae. Moreover, we investigated if molecules that exhibit antimicrobial activity were able to inhibit the proteolytic activity of the bacterium. Area of study: Isolates S1 and S2 were from Balcarce, Buenos Aires province, strain S3 from Rio Cuarto, Cordoba province, strain S4 from Concordia, Entre Rios province, strain S5 and S8 from Necochea, Buenos Aires, strain S6 and S7 from Mar del Plata, Buenos Aires, strain S9 from Modena, Italy and strain S10 from Emilia Reggio, Italy. Material and methods: Bacterial isolates identification was carried out by amplification of a specific 16S rRNA gene fragment of P. larvae using primers PL5 and PL4. Screening of the antimicrobial activity of thirteen molecules against four P. larvae isolates was conducted by the agar diffusion technique. The antimicrobial activity of selected molecules was evaluated by broth microdilution method. Main results: Menadione, lauric acid, monoglyceride of lauric acid and naringenin showed antimicrobial activity against ten P. larvae isolates. Menadione and lauric acid showed the strongest activities, with minimum inhibitory concentration mean values ranging 0.78-3.125 µg/mL and 25-50 µg/mL, respectively. Research highlights: Those concentrations are feasible to be applied at field level, and constitute promissory candidates to be evaluated using in vivo larval models.
Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
Fil: Cugnata, Noelia Melina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
Fil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Arredondo, Daniela. Instituto de Investigaciones Biologicas Clemente Estable, Montevideo; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biologicas Clemente Estable, Montevideo; Uruguay
Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
Materia
NATURAL MOLECULES
PAENIBACILLUS LARVAE
AMERICAN FOULBROOD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/122714
Acceder
id CONICETDig_a8122326c8284931c1371d4d8a247e94
oai_identifier_str oai:ri.conicet.gov.ar:11336/122714
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)Giménez Martínez, Pablo DarianCugnata, Noelia MelinaAlonso Salces, Rosa MariaArredondo, DanielaAntúnez, Karinade Castro, Rosana EstherFuselli, Sandra RosaNATURAL MOLECULESPAENIBACILLUS LARVAEAMERICAN FOULBROODhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aim of study: To evaluate the potential bactericidal activity of natural molecules against Paenibacillus larvae. Moreover, we investigated if molecules that exhibit antimicrobial activity were able to inhibit the proteolytic activity of the bacterium. Area of study: Isolates S1 and S2 were from Balcarce, Buenos Aires province, strain S3 from Rio Cuarto, Cordoba province, strain S4 from Concordia, Entre Rios province, strain S5 and S8 from Necochea, Buenos Aires, strain S6 and S7 from Mar del Plata, Buenos Aires, strain S9 from Modena, Italy and strain S10 from Emilia Reggio, Italy. Material and methods: Bacterial isolates identification was carried out by amplification of a specific 16S rRNA gene fragment of P. larvae using primers PL5 and PL4. Screening of the antimicrobial activity of thirteen molecules against four P. larvae isolates was conducted by the agar diffusion technique. The antimicrobial activity of selected molecules was evaluated by broth microdilution method. Main results: Menadione, lauric acid, monoglyceride of lauric acid and naringenin showed antimicrobial activity against ten P. larvae isolates. Menadione and lauric acid showed the strongest activities, with minimum inhibitory concentration mean values ranging 0.78-3.125 µg/mL and 25-50 µg/mL, respectively. Research highlights: Those concentrations are feasible to be applied at field level, and constitute promissory candidates to be evaluated using in vivo larval models.Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; ArgentinaFil: Cugnata, Noelia Melina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; ArgentinaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Arredondo, Daniela. Instituto de Investigaciones Biologicas Clemente Estable, Montevideo; UruguayFil: Antúnez, Karina. Instituto de Investigaciones Biologicas Clemente Estable, Montevideo; UruguayFil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; ArgentinaSpanish National Institute for Agriculture and Food Research and Technology2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122714Giménez Martínez, Pablo Darian; Cugnata, Noelia Melina; Alonso Salces, Rosa Maria; Arredondo, Daniela; Antúnez, Karina; et al.; Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.); Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 17; 3; 10-2019; 1-71695-971X2171-9292CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2019173-14740info:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/14740info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:27Zoai:ri.conicet.gov.ar:11336/122714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:27.952CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
title Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
spellingShingle Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
Giménez Martínez, Pablo Darian
NATURAL MOLECULES
PAENIBACILLUS LARVAE
AMERICAN FOULBROOD
title_short Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
title_full Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
title_fullStr Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
title_full_unstemmed Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
title_sort Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)
dc.creator.none.fl_str_mv Giménez Martínez, Pablo Darian
Cugnata, Noelia Melina
Alonso Salces, Rosa Maria
Arredondo, Daniela
Antúnez, Karina
de Castro, Rosana Esther
Fuselli, Sandra Rosa
author Giménez Martínez, Pablo Darian
author_facet Giménez Martínez, Pablo Darian
Cugnata, Noelia Melina
Alonso Salces, Rosa Maria
Arredondo, Daniela
Antúnez, Karina
de Castro, Rosana Esther
Fuselli, Sandra Rosa
author_role author
author2 Cugnata, Noelia Melina
Alonso Salces, Rosa Maria
Arredondo, Daniela
Antúnez, Karina
de Castro, Rosana Esther
Fuselli, Sandra Rosa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv NATURAL MOLECULES
PAENIBACILLUS LARVAE
AMERICAN FOULBROOD
topic NATURAL MOLECULES
PAENIBACILLUS LARVAE
AMERICAN FOULBROOD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aim of study: To evaluate the potential bactericidal activity of natural molecules against Paenibacillus larvae. Moreover, we investigated if molecules that exhibit antimicrobial activity were able to inhibit the proteolytic activity of the bacterium. Area of study: Isolates S1 and S2 were from Balcarce, Buenos Aires province, strain S3 from Rio Cuarto, Cordoba province, strain S4 from Concordia, Entre Rios province, strain S5 and S8 from Necochea, Buenos Aires, strain S6 and S7 from Mar del Plata, Buenos Aires, strain S9 from Modena, Italy and strain S10 from Emilia Reggio, Italy. Material and methods: Bacterial isolates identification was carried out by amplification of a specific 16S rRNA gene fragment of P. larvae using primers PL5 and PL4. Screening of the antimicrobial activity of thirteen molecules against four P. larvae isolates was conducted by the agar diffusion technique. The antimicrobial activity of selected molecules was evaluated by broth microdilution method. Main results: Menadione, lauric acid, monoglyceride of lauric acid and naringenin showed antimicrobial activity against ten P. larvae isolates. Menadione and lauric acid showed the strongest activities, with minimum inhibitory concentration mean values ranging 0.78-3.125 µg/mL and 25-50 µg/mL, respectively. Research highlights: Those concentrations are feasible to be applied at field level, and constitute promissory candidates to be evaluated using in vivo larval models.
Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
Fil: Cugnata, Noelia Melina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
Fil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Arredondo, Daniela. Instituto de Investigaciones Biologicas Clemente Estable, Montevideo; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biologicas Clemente Estable, Montevideo; Uruguay
Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
description Aim of study: To evaluate the potential bactericidal activity of natural molecules against Paenibacillus larvae. Moreover, we investigated if molecules that exhibit antimicrobial activity were able to inhibit the proteolytic activity of the bacterium. Area of study: Isolates S1 and S2 were from Balcarce, Buenos Aires province, strain S3 from Rio Cuarto, Cordoba province, strain S4 from Concordia, Entre Rios province, strain S5 and S8 from Necochea, Buenos Aires, strain S6 and S7 from Mar del Plata, Buenos Aires, strain S9 from Modena, Italy and strain S10 from Emilia Reggio, Italy. Material and methods: Bacterial isolates identification was carried out by amplification of a specific 16S rRNA gene fragment of P. larvae using primers PL5 and PL4. Screening of the antimicrobial activity of thirteen molecules against four P. larvae isolates was conducted by the agar diffusion technique. The antimicrobial activity of selected molecules was evaluated by broth microdilution method. Main results: Menadione, lauric acid, monoglyceride of lauric acid and naringenin showed antimicrobial activity against ten P. larvae isolates. Menadione and lauric acid showed the strongest activities, with minimum inhibitory concentration mean values ranging 0.78-3.125 µg/mL and 25-50 µg/mL, respectively. Research highlights: Those concentrations are feasible to be applied at field level, and constitute promissory candidates to be evaluated using in vivo larval models.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122714
Giménez Martínez, Pablo Darian; Cugnata, Noelia Melina; Alonso Salces, Rosa Maria; Arredondo, Daniela; Antúnez, Karina; et al.; Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.); Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 17; 3; 10-2019; 1-7
1695-971X
2171-9292
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122714
identifier_str_mv Giménez Martínez, Pablo Darian; Cugnata, Noelia Melina; Alonso Salces, Rosa Maria; Arredondo, Daniela; Antúnez, Karina; et al.; Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.); Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 17; 3; 10-2019; 1-7
1695-971X
2171-9292
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2019173-14740
info:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/14740
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268603267678208
score 13.13397

Publicaciones similares