Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry

Autores
Tascon, Marcos; Benavente, Fernando; Sanz Nebot, Victoria M.; Gagliardi, Leonardo Gabriel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of algae as a foodstuff is rapidly expanding worldwide from the East Asian countries, where they are also used for medical care. Harmala alkaloids (HAlk) are a family of bioactive compounds found in the extracts of some plants, including wakame (Undaria pinnatifida), an edible marine invasive algae. HAlks are based on a characteristic β-carboline structure with at least one amino ionizable group. In this work, we report the successful separation of a mixture of six HAlks (harmine, harmaline, harmol, harmalol, harmane, and norharmane) by capillary electrophoresis ion-trap mass spectrometry (CE-IT-MS) in less than 8 min. Optimum separation in fused-silica capillaries and detection sensitivity in positive-ion mode were achieved using a background electrolyte (BGE) with 25 mmol L−1 ammonium acetate (pH 7.8) and 10 % (v/v) methanol, and a sheath liquid with 60:40 (v/v) isopropanol–water and 0.05 % (v/v) formic acid. The separation method was validated in terms of linearity, limits of detection and quantification, repeatability, and reproducibility. Later, a sample pretreatment was carefully optimized to determine HAlks in commercial wakame samples with excellent recovery and repeatability. For the complex wakame extracts, the MS–MS fragmentation patterns of the different HAlks were useful to ensure a reliable identification. The complete procedure was validated using the standard-addition calibration method, determining matrix effects on the studied compounds. Harmalol, harmine, and harmaline were naturally present in the samples and were quantified at very low concentrations, ranging from 7 to 24 μg kg−1 dry algae.
Fil: Tascon, Marcos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benavente, Fernando. Universidad de Barcelona; España
Fil: Sanz Nebot, Victoria M.. Universidad de Barcelona; España
Fil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Tecnología de Pinturas (i); Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
Capillary Electrophoresis
Ms-Ms
Undaria Pinnatifida
Harmala Alkaloids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13717
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13717
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometryTascon, MarcosBenavente, FernandoSanz Nebot, Victoria M.Gagliardi, Leonardo GabrielCapillary ElectrophoresisMs-MsUndaria PinnatifidaHarmala Alkaloidshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The use of algae as a foodstuff is rapidly expanding worldwide from the East Asian countries, where they are also used for medical care. Harmala alkaloids (HAlk) are a family of bioactive compounds found in the extracts of some plants, including wakame (Undaria pinnatifida), an edible marine invasive algae. HAlks are based on a characteristic β-carboline structure with at least one amino ionizable group. In this work, we report the successful separation of a mixture of six HAlks (harmine, harmaline, harmol, harmalol, harmane, and norharmane) by capillary electrophoresis ion-trap mass spectrometry (CE-IT-MS) in less than 8 min. Optimum separation in fused-silica capillaries and detection sensitivity in positive-ion mode were achieved using a background electrolyte (BGE) with 25 mmol L−1 ammonium acetate (pH 7.8) and 10 % (v/v) methanol, and a sheath liquid with 60:40 (v/v) isopropanol–water and 0.05 % (v/v) formic acid. The separation method was validated in terms of linearity, limits of detection and quantification, repeatability, and reproducibility. Later, a sample pretreatment was carefully optimized to determine HAlks in commercial wakame samples with excellent recovery and repeatability. For the complex wakame extracts, the MS–MS fragmentation patterns of the different HAlks were useful to ensure a reliable identification. The complete procedure was validated using the standard-addition calibration method, determining matrix effects on the studied compounds. Harmalol, harmine, and harmaline were naturally present in the samples and were quantified at very low concentrations, ranging from 7 to 24 μg kg−1 dry algae.Fil: Tascon, Marcos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Benavente, Fernando. Universidad de Barcelona; EspañaFil: Sanz Nebot, Victoria M.. Universidad de Barcelona; EspañaFil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Tecnología de Pinturas (i); Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaSpringer Heidelberg2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13717Tascon, Marcos; Benavente, Fernando; Sanz Nebot, Victoria M.; Gagliardi, Leonardo Gabriel; Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry; Springer Heidelberg; Analytical And Bioanalytical Chemistry; 407; 13; 5-2015; 3637-36451618-26421618-2650enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-015-8579-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00216-015-8579-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:42Zoai:ri.conicet.gov.ar:11336/13717instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:43.071CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
spellingShingle Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
Tascon, Marcos
Capillary Electrophoresis
Ms-Ms
Undaria Pinnatifida
Harmala Alkaloids
title_short Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_full Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_fullStr Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_full_unstemmed Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_sort Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
dc.creator.none.fl_str_mv Tascon, Marcos
Benavente, Fernando
Sanz Nebot, Victoria M.
Gagliardi, Leonardo Gabriel
author Tascon, Marcos
author_facet Tascon, Marcos
Benavente, Fernando
Sanz Nebot, Victoria M.
Gagliardi, Leonardo Gabriel
author_role author
author2 Benavente, Fernando
Sanz Nebot, Victoria M.
Gagliardi, Leonardo Gabriel
author2_role author
author
author
dc.subject.none.fl_str_mv Capillary Electrophoresis
Ms-Ms
Undaria Pinnatifida
Harmala Alkaloids
topic Capillary Electrophoresis
Ms-Ms
Undaria Pinnatifida
Harmala Alkaloids
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of algae as a foodstuff is rapidly expanding worldwide from the East Asian countries, where they are also used for medical care. Harmala alkaloids (HAlk) are a family of bioactive compounds found in the extracts of some plants, including wakame (Undaria pinnatifida), an edible marine invasive algae. HAlks are based on a characteristic β-carboline structure with at least one amino ionizable group. In this work, we report the successful separation of a mixture of six HAlks (harmine, harmaline, harmol, harmalol, harmane, and norharmane) by capillary electrophoresis ion-trap mass spectrometry (CE-IT-MS) in less than 8 min. Optimum separation in fused-silica capillaries and detection sensitivity in positive-ion mode were achieved using a background electrolyte (BGE) with 25 mmol L−1 ammonium acetate (pH 7.8) and 10 % (v/v) methanol, and a sheath liquid with 60:40 (v/v) isopropanol–water and 0.05 % (v/v) formic acid. The separation method was validated in terms of linearity, limits of detection and quantification, repeatability, and reproducibility. Later, a sample pretreatment was carefully optimized to determine HAlks in commercial wakame samples with excellent recovery and repeatability. For the complex wakame extracts, the MS–MS fragmentation patterns of the different HAlks were useful to ensure a reliable identification. The complete procedure was validated using the standard-addition calibration method, determining matrix effects on the studied compounds. Harmalol, harmine, and harmaline were naturally present in the samples and were quantified at very low concentrations, ranging from 7 to 24 μg kg−1 dry algae.
Fil: Tascon, Marcos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benavente, Fernando. Universidad de Barcelona; España
Fil: Sanz Nebot, Victoria M.. Universidad de Barcelona; España
Fil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Tecnología de Pinturas (i); Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description The use of algae as a foodstuff is rapidly expanding worldwide from the East Asian countries, where they are also used for medical care. Harmala alkaloids (HAlk) are a family of bioactive compounds found in the extracts of some plants, including wakame (Undaria pinnatifida), an edible marine invasive algae. HAlks are based on a characteristic β-carboline structure with at least one amino ionizable group. In this work, we report the successful separation of a mixture of six HAlks (harmine, harmaline, harmol, harmalol, harmane, and norharmane) by capillary electrophoresis ion-trap mass spectrometry (CE-IT-MS) in less than 8 min. Optimum separation in fused-silica capillaries and detection sensitivity in positive-ion mode were achieved using a background electrolyte (BGE) with 25 mmol L−1 ammonium acetate (pH 7.8) and 10 % (v/v) methanol, and a sheath liquid with 60:40 (v/v) isopropanol–water and 0.05 % (v/v) formic acid. The separation method was validated in terms of linearity, limits of detection and quantification, repeatability, and reproducibility. Later, a sample pretreatment was carefully optimized to determine HAlks in commercial wakame samples with excellent recovery and repeatability. For the complex wakame extracts, the MS–MS fragmentation patterns of the different HAlks were useful to ensure a reliable identification. The complete procedure was validated using the standard-addition calibration method, determining matrix effects on the studied compounds. Harmalol, harmine, and harmaline were naturally present in the samples and were quantified at very low concentrations, ranging from 7 to 24 μg kg−1 dry algae.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13717
Tascon, Marcos; Benavente, Fernando; Sanz Nebot, Victoria M.; Gagliardi, Leonardo Gabriel; Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry; Springer Heidelberg; Analytical And Bioanalytical Chemistry; 407; 13; 5-2015; 3637-3645
1618-2642
1618-2650
url http://hdl.handle.net/11336/13717
identifier_str_mv Tascon, Marcos; Benavente, Fernando; Sanz Nebot, Victoria M.; Gagliardi, Leonardo Gabriel; Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry; Springer Heidelberg; Analytical And Bioanalytical Chemistry; 407; 13; 5-2015; 3637-3645
1618-2642
1618-2650
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-015-8579-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00216-015-8579-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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