Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids

Autores
Tascon, Marcos; Benavente, Fernando; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In capillary electrophoresis (CE), resolution (Rs) and selectivity (α) are criteria often used in practice to optimize separations. Nevertheless, when these and other proposed parameters are considered as an elementary criterion for optimization by mathematical maximization, certain issues and inconsistencies appear. In the present work we analyzed the pros and cons of using these parameters as elementary criteria for mathematical optimization of capillary electrophoretic separations. We characterized the requirements of an ideal criterion to qualify separations within the framework of mathematical optimizations and, accordingly, propose: -1- a new elementary criterion (t') and -2- a method to extend this elementary criterion to compose a global function that simultaneously qualifies many different aspects, also called multicriteria optimization function (MCOF). In order to demonstrate this new concept, we employed a group of six alkaloids with closely related structures (harmine, harmaline, harmol, harmalol, harmane and norharmane). On the basis of this system, we present a critical comparison between the new optimization criterion t' and the former elementary criteria. Finally, aimed at validating the proposed methods, we composed an MCOF in which the capillary-electrophoretic separation of the six model compounds is mathematically optimized as a function of pH as the unique variable. Experimental results subsequently confirmed the accuracy of the model.
Fil: Tascon, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Benavente, Fernando. Universidad de Barcelona; España
Fil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
CAPILLARY ELECTROPHORESIS
HARMALA ALKALOIDS
OPTIMIZATION
QUALITY CRITERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54838
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloidsTascon, MarcosBenavente, FernandoCastells, Cecilia Beatriz MartaGagliardi, Leonardo GabrielCAPILLARY ELECTROPHORESISHARMALA ALKALOIDSOPTIMIZATIONQUALITY CRITERIAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In capillary electrophoresis (CE), resolution (Rs) and selectivity (α) are criteria often used in practice to optimize separations. Nevertheless, when these and other proposed parameters are considered as an elementary criterion for optimization by mathematical maximization, certain issues and inconsistencies appear. In the present work we analyzed the pros and cons of using these parameters as elementary criteria for mathematical optimization of capillary electrophoretic separations. We characterized the requirements of an ideal criterion to qualify separations within the framework of mathematical optimizations and, accordingly, propose: -1- a new elementary criterion (t') and -2- a method to extend this elementary criterion to compose a global function that simultaneously qualifies many different aspects, also called multicriteria optimization function (MCOF). In order to demonstrate this new concept, we employed a group of six alkaloids with closely related structures (harmine, harmaline, harmol, harmalol, harmane and norharmane). On the basis of this system, we present a critical comparison between the new optimization criterion t' and the former elementary criteria. Finally, aimed at validating the proposed methods, we composed an MCOF in which the capillary-electrophoretic separation of the six model compounds is mathematically optimized as a function of pH as the unique variable. Experimental results subsequently confirmed the accuracy of the model.Fil: Tascon, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Benavente, Fernando. Universidad de Barcelona; EspañaFil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaElsevier Science2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54838Tascon, Marcos; Benavente, Fernando; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel; Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids; Elsevier Science; Journal of Chromatography - A; 1460; 8-2016; 190-1960021-9673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2016.07.032info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021967316309396info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:11Zoai:ri.conicet.gov.ar:11336/54838instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:11.481CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
title Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
spellingShingle Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
Tascon, Marcos
CAPILLARY ELECTROPHORESIS
HARMALA ALKALOIDS
OPTIMIZATION
QUALITY CRITERIA
title_short Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
title_full Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
title_fullStr Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
title_full_unstemmed Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
title_sort Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids
dc.creator.none.fl_str_mv Tascon, Marcos
Benavente, Fernando
Castells, Cecilia Beatriz Marta
Gagliardi, Leonardo Gabriel
author Tascon, Marcos
author_facet Tascon, Marcos
Benavente, Fernando
Castells, Cecilia Beatriz Marta
Gagliardi, Leonardo Gabriel
author_role author
author2 Benavente, Fernando
Castells, Cecilia Beatriz Marta
Gagliardi, Leonardo Gabriel
author2_role author
author
author
dc.subject.none.fl_str_mv CAPILLARY ELECTROPHORESIS
HARMALA ALKALOIDS
OPTIMIZATION
QUALITY CRITERIA
topic CAPILLARY ELECTROPHORESIS
HARMALA ALKALOIDS
OPTIMIZATION
QUALITY CRITERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In capillary electrophoresis (CE), resolution (Rs) and selectivity (α) are criteria often used in practice to optimize separations. Nevertheless, when these and other proposed parameters are considered as an elementary criterion for optimization by mathematical maximization, certain issues and inconsistencies appear. In the present work we analyzed the pros and cons of using these parameters as elementary criteria for mathematical optimization of capillary electrophoretic separations. We characterized the requirements of an ideal criterion to qualify separations within the framework of mathematical optimizations and, accordingly, propose: -1- a new elementary criterion (t') and -2- a method to extend this elementary criterion to compose a global function that simultaneously qualifies many different aspects, also called multicriteria optimization function (MCOF). In order to demonstrate this new concept, we employed a group of six alkaloids with closely related structures (harmine, harmaline, harmol, harmalol, harmane and norharmane). On the basis of this system, we present a critical comparison between the new optimization criterion t' and the former elementary criteria. Finally, aimed at validating the proposed methods, we composed an MCOF in which the capillary-electrophoretic separation of the six model compounds is mathematically optimized as a function of pH as the unique variable. Experimental results subsequently confirmed the accuracy of the model.
Fil: Tascon, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Benavente, Fernando. Universidad de Barcelona; España
Fil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description In capillary electrophoresis (CE), resolution (Rs) and selectivity (α) are criteria often used in practice to optimize separations. Nevertheless, when these and other proposed parameters are considered as an elementary criterion for optimization by mathematical maximization, certain issues and inconsistencies appear. In the present work we analyzed the pros and cons of using these parameters as elementary criteria for mathematical optimization of capillary electrophoretic separations. We characterized the requirements of an ideal criterion to qualify separations within the framework of mathematical optimizations and, accordingly, propose: -1- a new elementary criterion (t') and -2- a method to extend this elementary criterion to compose a global function that simultaneously qualifies many different aspects, also called multicriteria optimization function (MCOF). In order to demonstrate this new concept, we employed a group of six alkaloids with closely related structures (harmine, harmaline, harmol, harmalol, harmane and norharmane). On the basis of this system, we present a critical comparison between the new optimization criterion t' and the former elementary criteria. Finally, aimed at validating the proposed methods, we composed an MCOF in which the capillary-electrophoretic separation of the six model compounds is mathematically optimized as a function of pH as the unique variable. Experimental results subsequently confirmed the accuracy of the model.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54838
Tascon, Marcos; Benavente, Fernando; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel; Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids; Elsevier Science; Journal of Chromatography - A; 1460; 8-2016; 190-196
0021-9673
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54838
identifier_str_mv Tascon, Marcos; Benavente, Fernando; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel; Quality criterion to optimize separations in capillary electrophoresis: Application to the analysis of harmala alkaloids; Elsevier Science; Journal of Chromatography - A; 1460; 8-2016; 190-196
0021-9673
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2016.07.032
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021967316309396
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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