Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization

Autores
de Antoni, Graciela; Zago, Miriam; Vasek, Olga M.; Giraffa, Giorgio; Carminati, D.; Briggiler Marcó, Mariángeles; Reinheimer, Jorge Alberto; Suárez, Viviana Beatriz
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two greatly related Lactobacillus plantarum bacteriophages (named FAGK1 and FAGK2) were isolated from Kefir grains of different origins. Both phages belonged to the Siphoviridae family (morphotype B1) and showed similar dimensions for head and tail sizes. The host range of the two phages, using 36 strains as potential host strains, differed only in the phage reactivity against one of them. The phages showed latent periods of 30 min, burst periods of 80 ± 10 min and burst size values of 11.0 ± 1.0 PFU per infected cell as mean value. Identical DNA restriction patterns were obtained for both phages with PvuI, SalI, HindIII and MluI. The viral DNA apparently did not present extremes cos and the structural protein patterns presented four major bands (32.9, 35.7, 43.0 and 66.2 kDa).             This study reports the first isolation of bacteriophages of Lb. plantarum from Kefir grains and adds further knowledge regarding the complex microbial community of this fermented milk.
Fil: de Antoni, Graciela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zago, Miriam. Research Centre for Forage and Dairy Productions; Italia
Fil: Vasek, Olga M.. Universidad Nacional del Nordeste; Argentina
Fil: Giraffa, Giorgio. Research Centre for Forage and Dairy Productions; Italia
Fil: Carminati, D.. Research Centre for Forage and Dairy Productions; Italia
Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
BACTERIOPHAGES
Lactobacillus plantarum
KEFIR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103197
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/103197
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterizationde Antoni, GracielaZago, MiriamVasek, Olga M.Giraffa, GiorgioCarminati, D.Briggiler Marcó, MariángelesReinheimer, Jorge AlbertoSuárez, Viviana BeatrizBACTERIOPHAGESLactobacillus plantarumKEFIRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two greatly related Lactobacillus plantarum bacteriophages (named FAGK1 and FAGK2) were isolated from Kefir grains of different origins. Both phages belonged to the Siphoviridae family (morphotype B1) and showed similar dimensions for head and tail sizes. The host range of the two phages, using 36 strains as potential host strains, differed only in the phage reactivity against one of them. The phages showed latent periods of 30 min, burst periods of 80 ± 10 min and burst size values of 11.0 ± 1.0 PFU per infected cell as mean value. Identical DNA restriction patterns were obtained for both phages with PvuI, SalI, HindIII and MluI. The viral DNA apparently did not present extremes cos and the structural protein patterns presented four major bands (32.9, 35.7, 43.0 and 66.2 kDa).             This study reports the first isolation of bacteriophages of Lb. plantarum from Kefir grains and adds further knowledge regarding the complex microbial community of this fermented milk.Fil: de Antoni, Graciela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Zago, Miriam. Research Centre for Forage and Dairy Productions; ItaliaFil: Vasek, Olga M.. Universidad Nacional del Nordeste; ArgentinaFil: Giraffa, Giorgio. Research Centre for Forage and Dairy Productions; ItaliaFil: Carminati, D.. Research Centre for Forage and Dairy Productions; ItaliaFil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaCambridge University Press2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103197de Antoni, Graciela; Zago, Miriam; Vasek, Olga M.; Giraffa, Giorgio; Carminati, D.; et al.; Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization; Cambridge University Press; Journal of Dairy Research; 77; 12-2010; 7-120022-0299CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029909990203info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:36Zoai:ri.conicet.gov.ar:11336/103197instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:36.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
title Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
spellingShingle Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
de Antoni, Graciela
BACTERIOPHAGES
Lactobacillus plantarum
KEFIR
title_short Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
title_full Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
title_fullStr Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
title_full_unstemmed Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
title_sort Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization
dc.creator.none.fl_str_mv de Antoni, Graciela
Zago, Miriam
Vasek, Olga M.
Giraffa, Giorgio
Carminati, D.
Briggiler Marcó, Mariángeles
Reinheimer, Jorge Alberto
Suárez, Viviana Beatriz
author de Antoni, Graciela
author_facet de Antoni, Graciela
Zago, Miriam
Vasek, Olga M.
Giraffa, Giorgio
Carminati, D.
Briggiler Marcó, Mariángeles
Reinheimer, Jorge Alberto
Suárez, Viviana Beatriz
author_role author
author2 Zago, Miriam
Vasek, Olga M.
Giraffa, Giorgio
Carminati, D.
Briggiler Marcó, Mariángeles
Reinheimer, Jorge Alberto
Suárez, Viviana Beatriz
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIOPHAGES
Lactobacillus plantarum
KEFIR
topic BACTERIOPHAGES
Lactobacillus plantarum
KEFIR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two greatly related Lactobacillus plantarum bacteriophages (named FAGK1 and FAGK2) were isolated from Kefir grains of different origins. Both phages belonged to the Siphoviridae family (morphotype B1) and showed similar dimensions for head and tail sizes. The host range of the two phages, using 36 strains as potential host strains, differed only in the phage reactivity against one of them. The phages showed latent periods of 30 min, burst periods of 80 ± 10 min and burst size values of 11.0 ± 1.0 PFU per infected cell as mean value. Identical DNA restriction patterns were obtained for both phages with PvuI, SalI, HindIII and MluI. The viral DNA apparently did not present extremes cos and the structural protein patterns presented four major bands (32.9, 35.7, 43.0 and 66.2 kDa).             This study reports the first isolation of bacteriophages of Lb. plantarum from Kefir grains and adds further knowledge regarding the complex microbial community of this fermented milk.
Fil: de Antoni, Graciela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zago, Miriam. Research Centre for Forage and Dairy Productions; Italia
Fil: Vasek, Olga M.. Universidad Nacional del Nordeste; Argentina
Fil: Giraffa, Giorgio. Research Centre for Forage and Dairy Productions; Italia
Fil: Carminati, D.. Research Centre for Forage and Dairy Productions; Italia
Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description Two greatly related Lactobacillus plantarum bacteriophages (named FAGK1 and FAGK2) were isolated from Kefir grains of different origins. Both phages belonged to the Siphoviridae family (morphotype B1) and showed similar dimensions for head and tail sizes. The host range of the two phages, using 36 strains as potential host strains, differed only in the phage reactivity against one of them. The phages showed latent periods of 30 min, burst periods of 80 ± 10 min and burst size values of 11.0 ± 1.0 PFU per infected cell as mean value. Identical DNA restriction patterns were obtained for both phages with PvuI, SalI, HindIII and MluI. The viral DNA apparently did not present extremes cos and the structural protein patterns presented four major bands (32.9, 35.7, 43.0 and 66.2 kDa).             This study reports the first isolation of bacteriophages of Lb. plantarum from Kefir grains and adds further knowledge regarding the complex microbial community of this fermented milk.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103197
de Antoni, Graciela; Zago, Miriam; Vasek, Olga M.; Giraffa, Giorgio; Carminati, D.; et al.; Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization; Cambridge University Press; Journal of Dairy Research; 77; 12-2010; 7-12
0022-0299
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103197
identifier_str_mv de Antoni, Graciela; Zago, Miriam; Vasek, Olga M.; Giraffa, Giorgio; Carminati, D.; et al.; Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization; Cambridge University Press; Journal of Dairy Research; 77; 12-2010; 7-12
0022-0299
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029909990203
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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