Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages

Autores
Briggiler Marcó, Mariángeles; de Antoni, Graciela Liliana; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of several biocides, thermal treatments, and photocatalysis on the viability of four Lactobacillus plantarum phages was investigated. Times to achieve 99% inactivation (T99) of phages at 63, 72, and 90°C were evaluated in four  suspension media: deMan Rogosa Sharpe broth, reconstituted skim milk, a commercial EM-glucose medium, and Tris magnesium  gelatin buffer. The four phages studied were highly resistant to 63°C (T99 > 45 min); however, counts < 10 PFU/ml  were achieved by heating at 90°C for 5 min. Higher thermal resistance at 72°C was observed when reconstituted skim milk  and EM-glucose medium were assayed. Peracetic acid (0.15%, vol/vol) was an effective biocide for the complete inactivation  of all phages studied within 5 min of exposure. Sodium hypochlorite (800 ppm) inactivated the phages completely within 30 min. Ethanol (100%) did not destroy phage particles even after 45 min. Isopropanol did not have any effect on phage viability. Phage counts < 50 PFU/ml were obtained within 180 min of photocatalytic treatment. The results obtained in this work are  important for establishing adequate methods for inactivating phages in industrial plants and laboratory environments.
Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: de Antoni, Graciela Liliana. Universidad Nacional de La Plata; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
Lactobacillus plantarum
BACTERIOPHAGE INACTIVATION
HEAT TREATMENT
BIOCIDES
PHOTOCATALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101901
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/101901
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophagesBriggiler Marcó, Mariángelesde Antoni, Graciela LilianaReinheimer, Jorge AlbertoQuiberoni, Andrea del LujanLactobacillus plantarumBACTERIOPHAGE INACTIVATIONHEAT TREATMENTBIOCIDESPHOTOCATALYSIShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The effect of several biocides, thermal treatments, and photocatalysis on the viability of four Lactobacillus plantarum phages was investigated. Times to achieve 99% inactivation (T99) of phages at 63, 72, and 90°C were evaluated in four  suspension media: deMan Rogosa Sharpe broth, reconstituted skim milk, a commercial EM-glucose medium, and Tris magnesium  gelatin buffer. The four phages studied were highly resistant to 63°C (T99 > 45 min); however, counts < 10 PFU/ml  were achieved by heating at 90°C for 5 min. Higher thermal resistance at 72°C was observed when reconstituted skim milk  and EM-glucose medium were assayed. Peracetic acid (0.15%, vol/vol) was an effective biocide for the complete inactivation  of all phages studied within 5 min of exposure. Sodium hypochlorite (800 ppm) inactivated the phages completely within 30 min. Ethanol (100%) did not destroy phage particles even after 45 min. Isopropanol did not have any effect on phage viability. Phage counts < 50 PFU/ml were obtained within 180 min of photocatalytic treatment. The results obtained in this work are  important for establishing adequate methods for inactivating phages in industrial plants and laboratory environments.Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: de Antoni, Graciela Liliana. Universidad Nacional de La Plata; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaInternational Association for Food Protection2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101901Briggiler Marcó, Mariángeles; de Antoni, Graciela Liliana; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages; International Association for Food Protection; Journal of Food Protection; 72; 5; 5-2009; 1012-10190362-028XCONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:17Zoai:ri.conicet.gov.ar:11336/101901instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:17.456CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
title Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
spellingShingle Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
Briggiler Marcó, Mariángeles
Lactobacillus plantarum
BACTERIOPHAGE INACTIVATION
HEAT TREATMENT
BIOCIDES
PHOTOCATALYSIS
title_short Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
title_full Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
title_fullStr Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
title_full_unstemmed Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
title_sort Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
dc.creator.none.fl_str_mv Briggiler Marcó, Mariángeles
de Antoni, Graciela Liliana
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author Briggiler Marcó, Mariángeles
author_facet Briggiler Marcó, Mariángeles
de Antoni, Graciela Liliana
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author_role author
author2 de Antoni, Graciela Liliana
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author2_role author
author
author
dc.subject.none.fl_str_mv Lactobacillus plantarum
BACTERIOPHAGE INACTIVATION
HEAT TREATMENT
BIOCIDES
PHOTOCATALYSIS
topic Lactobacillus plantarum
BACTERIOPHAGE INACTIVATION
HEAT TREATMENT
BIOCIDES
PHOTOCATALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The effect of several biocides, thermal treatments, and photocatalysis on the viability of four Lactobacillus plantarum phages was investigated. Times to achieve 99% inactivation (T99) of phages at 63, 72, and 90°C were evaluated in four  suspension media: deMan Rogosa Sharpe broth, reconstituted skim milk, a commercial EM-glucose medium, and Tris magnesium  gelatin buffer. The four phages studied were highly resistant to 63°C (T99 > 45 min); however, counts < 10 PFU/ml  were achieved by heating at 90°C for 5 min. Higher thermal resistance at 72°C was observed when reconstituted skim milk  and EM-glucose medium were assayed. Peracetic acid (0.15%, vol/vol) was an effective biocide for the complete inactivation  of all phages studied within 5 min of exposure. Sodium hypochlorite (800 ppm) inactivated the phages completely within 30 min. Ethanol (100%) did not destroy phage particles even after 45 min. Isopropanol did not have any effect on phage viability. Phage counts < 50 PFU/ml were obtained within 180 min of photocatalytic treatment. The results obtained in this work are  important for establishing adequate methods for inactivating phages in industrial plants and laboratory environments.
Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: de Antoni, Graciela Liliana. Universidad Nacional de La Plata; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description The effect of several biocides, thermal treatments, and photocatalysis on the viability of four Lactobacillus plantarum phages was investigated. Times to achieve 99% inactivation (T99) of phages at 63, 72, and 90°C were evaluated in four  suspension media: deMan Rogosa Sharpe broth, reconstituted skim milk, a commercial EM-glucose medium, and Tris magnesium  gelatin buffer. The four phages studied were highly resistant to 63°C (T99 > 45 min); however, counts < 10 PFU/ml  were achieved by heating at 90°C for 5 min. Higher thermal resistance at 72°C was observed when reconstituted skim milk  and EM-glucose medium were assayed. Peracetic acid (0.15%, vol/vol) was an effective biocide for the complete inactivation  of all phages studied within 5 min of exposure. Sodium hypochlorite (800 ppm) inactivated the phages completely within 30 min. Ethanol (100%) did not destroy phage particles even after 45 min. Isopropanol did not have any effect on phage viability. Phage counts < 50 PFU/ml were obtained within 180 min of photocatalytic treatment. The results obtained in this work are  important for establishing adequate methods for inactivating phages in industrial plants and laboratory environments.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101901
Briggiler Marcó, Mariángeles; de Antoni, Graciela Liliana; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages; International Association for Food Protection; Journal of Food Protection; 72; 5; 5-2009; 1012-1019
0362-028X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101901
identifier_str_mv Briggiler Marcó, Mariángeles; de Antoni, Graciela Liliana; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages; International Association for Food Protection; Journal of Food Protection; 72; 5; 5-2009; 1012-1019
0362-028X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Association for Food Protection
publisher.none.fl_str_mv International Association for Food Protection
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842979870710169600
score 12.48226

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