Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion

Autores
Kakisu, Emiliano Javier; Bolla, Patricia Araceli; Abraham, Analia Graciela; De Urraza, Patricio; De Antoni, Graciela L.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two types of microbial mixtures containing selected lactic acid bacteria and yeasts isolated from kefir grains were tested against the Shigella invasion of Hep-2 cells in culture. A five-strain mixture demonstrated a significant inhibition of the cell internalisation of Shigella flexneri and Shigella sonnei. The addition of single kefir strains or their mixtures to the cells, before the addition of Shigella, protected more efficiently than the simultaneous addition of the lactic-acid bacteria and Shigella on the cells. Among the kefir strains assayed, Lactobacillus plantarum strain CIDCA 83114 showed the most significant inhibition of the invasion. Both the cell walls of L. plantarum and the intact bacterial cells demonstrated an equivalent protection of cell monolayers; with the possibility of a protein or peptide mediating this effect. These results provide evidence for the potential inhibitory properties of certain kefir strains, such as L. plantarum CIDCA 83114, against disease-producing species of Shigella.
Fil: Kakisu, Emiliano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Bolla, Patricia Araceli. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Abraham, Analia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: De Urraza, Patricio. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: De Antoni, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
Lactobacillus Plantarum
Shigella
Hep-2
Kefir
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10248
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10248
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasionKakisu, Emiliano JavierBolla, Patricia AraceliAbraham, Analia GracielaDe Urraza, PatricioDe Antoni, Graciela L.Lactobacillus PlantarumShigellaHep-2Kefirhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two types of microbial mixtures containing selected lactic acid bacteria and yeasts isolated from kefir grains were tested against the Shigella invasion of Hep-2 cells in culture. A five-strain mixture demonstrated a significant inhibition of the cell internalisation of Shigella flexneri and Shigella sonnei. The addition of single kefir strains or their mixtures to the cells, before the addition of Shigella, protected more efficiently than the simultaneous addition of the lactic-acid bacteria and Shigella on the cells. Among the kefir strains assayed, Lactobacillus plantarum strain CIDCA 83114 showed the most significant inhibition of the invasion. Both the cell walls of L. plantarum and the intact bacterial cells demonstrated an equivalent protection of cell monolayers; with the possibility of a protein or peptide mediating this effect. These results provide evidence for the potential inhibitory properties of certain kefir strains, such as L. plantarum CIDCA 83114, against disease-producing species of Shigella.Fil: Kakisu, Emiliano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Bolla, Patricia Araceli. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Abraham, Analia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: De Urraza, Patricio. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaFil: De Antoni, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaElsevier2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10248Kakisu, Emiliano Javier; Bolla, Patricia Araceli; Abraham, Analia Graciela; De Urraza, Patricio; De Antoni, Graciela L.; Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion; Elsevier; International Dairy Journal; 33; 1; 6-2013; 22-260958-6946enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2013.06.004info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694613001568info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:58Zoai:ri.conicet.gov.ar:11336/10248instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:58.628CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
title Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
spellingShingle Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
Kakisu, Emiliano Javier
Lactobacillus Plantarum
Shigella
Hep-2
Kefir
title_short Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
title_full Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
title_fullStr Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
title_full_unstemmed Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
title_sort Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion
dc.creator.none.fl_str_mv Kakisu, Emiliano Javier
Bolla, Patricia Araceli
Abraham, Analia Graciela
De Urraza, Patricio
De Antoni, Graciela L.
author Kakisu, Emiliano Javier
author_facet Kakisu, Emiliano Javier
Bolla, Patricia Araceli
Abraham, Analia Graciela
De Urraza, Patricio
De Antoni, Graciela L.
author_role author
author2 Bolla, Patricia Araceli
Abraham, Analia Graciela
De Urraza, Patricio
De Antoni, Graciela L.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Plantarum
Shigella
Hep-2
Kefir
topic Lactobacillus Plantarum
Shigella
Hep-2
Kefir
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two types of microbial mixtures containing selected lactic acid bacteria and yeasts isolated from kefir grains were tested against the Shigella invasion of Hep-2 cells in culture. A five-strain mixture demonstrated a significant inhibition of the cell internalisation of Shigella flexneri and Shigella sonnei. The addition of single kefir strains or their mixtures to the cells, before the addition of Shigella, protected more efficiently than the simultaneous addition of the lactic-acid bacteria and Shigella on the cells. Among the kefir strains assayed, Lactobacillus plantarum strain CIDCA 83114 showed the most significant inhibition of the invasion. Both the cell walls of L. plantarum and the intact bacterial cells demonstrated an equivalent protection of cell monolayers; with the possibility of a protein or peptide mediating this effect. These results provide evidence for the potential inhibitory properties of certain kefir strains, such as L. plantarum CIDCA 83114, against disease-producing species of Shigella.
Fil: Kakisu, Emiliano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Bolla, Patricia Araceli. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Abraham, Analia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: De Urraza, Patricio. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: De Antoni, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description Two types of microbial mixtures containing selected lactic acid bacteria and yeasts isolated from kefir grains were tested against the Shigella invasion of Hep-2 cells in culture. A five-strain mixture demonstrated a significant inhibition of the cell internalisation of Shigella flexneri and Shigella sonnei. The addition of single kefir strains or their mixtures to the cells, before the addition of Shigella, protected more efficiently than the simultaneous addition of the lactic-acid bacteria and Shigella on the cells. Among the kefir strains assayed, Lactobacillus plantarum strain CIDCA 83114 showed the most significant inhibition of the invasion. Both the cell walls of L. plantarum and the intact bacterial cells demonstrated an equivalent protection of cell monolayers; with the possibility of a protein or peptide mediating this effect. These results provide evidence for the potential inhibitory properties of certain kefir strains, such as L. plantarum CIDCA 83114, against disease-producing species of Shigella.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10248
Kakisu, Emiliano Javier; Bolla, Patricia Araceli; Abraham, Analia Graciela; De Urraza, Patricio; De Antoni, Graciela L.; Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion; Elsevier; International Dairy Journal; 33; 1; 6-2013; 22-26
0958-6946
url http://hdl.handle.net/11336/10248
identifier_str_mv Kakisu, Emiliano Javier; Bolla, Patricia Araceli; Abraham, Analia Graciela; De Urraza, Patricio; De Antoni, Graciela L.; Lactobacillus plantarum isolated from kefir: Protection of cultured Hep-2 cells against Shigella invasion; Elsevier; International Dairy Journal; 33; 1; 6-2013; 22-26
0958-6946
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2013.06.004
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694613001568
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269984181452800
score 13.13397

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